本文選題：脂肪干細(xì)胞 + 萬古霉素　； 參考：《中南大學(xué)》2009年碩士論文

【摘要】： 目的:研究大鼠脂肪干細(xì)胞(ADSCs)在體外分離培養(yǎng)、誘導(dǎo)分化為成骨細(xì)胞及成脂細(xì)胞的能力,初步觀察不同濃度萬古霉素對其增殖、成骨分化的影響 方法:將大鼠ADSCs放入成脂誘導(dǎo)液中培養(yǎng),采用油紅O染色觀察其成脂分化能力;同時將大鼠ADSCs放入不同濃度的萬古霉素,以及添加了成骨誘導(dǎo)劑的培養(yǎng)液中培養(yǎng),采用MTT法、不同時相點測定ALP值、Von Kossa染色法觀察萬古霉素對ADSCs的成骨影響。 結(jié)果:SD大鼠ADSCs可在不同濃度萬古霉素溶液中生長,當(dāng)萬古霉素濃度≤800μg/ml時,萬古霉素對ADSC增殖無影響,當(dāng)濃度≥1000μg/ml時,才對ADSC的增殖有一定影響。萬古霉素≤1000μg/ml時,實驗組與對照組在不同時相點ALP無明顯差異。通過向成脂細(xì)胞及成骨細(xì)胞定向誘導(dǎo)后,油紅染色、堿性磷酸酶染色和vonKossa染色陽性。 結(jié)論:大鼠脂肪干細(xì)胞能向脂肪細(xì)胞和成骨細(xì)胞誘導(dǎo)分化;萬古霉素在一定濃度范圍內(nèi)對ADSC增殖、成骨分化影響較小,當(dāng)達(dá)到某一濃度高值時才對其產(chǎn)生影響,是預(yù)防和治療骨科臨床感染的較好選擇。
[Abstract]:Objective: to study the ability of rat adipose stem cells (ADSCs) to differentiate into osteoblasts and adipoblasts in vitro, and to observe the effects of vancomycin at different concentrations on the proliferation and osteogenic differentiation of adipose stem cells. Methods: rat ADSCs was cultured in fat-forming medium, and the ability of adipogenic differentiation was observed by oil red O staining, and rat ADSCs was cultured in different concentrations of vancomycin and medium supplemented with osteogenic inducer. MTT method was used. The effect of vancomycin on ADSCs osteogenesis was observed by Von Kossa staining at different phase points. Results ADSCs could grow in different concentrations of vancomycin solution. When vancomycin concentration 鈮，

本文編號：1976111