【摘要】：目的:觀察腦缺血再灌注損傷后早期運(yùn)動(dòng)訓(xùn)練對突觸再生的影響,探討Agrin在腦缺血后運(yùn)動(dòng)促進(jìn)突觸再生中的作用以及潛在的調(diào)控機(jī)制。方法:采用雄性SD大鼠,構(gòu)建60min的左側(cè)大腦中動(dòng)脈栓塞再灌注(middlecerebral artery occlusion,MCAO)模型。實(shí)驗(yàn)共分為三組:假手術(shù)組(Sham)、缺血未運(yùn)動(dòng)組(non-exercise group,NE)、缺血早期運(yùn)動(dòng)訓(xùn)練組(early exercise group,EE)。Sham 組和NE組大鼠不進(jìn)行訓(xùn)練,EE組大鼠在MCAO后24h介入運(yùn)動(dòng)訓(xùn)練強(qiáng)度逐步增強(qiáng)的跑步訓(xùn)練,每天30min,持續(xù)14d。在腦缺血再灌注損傷后相應(yīng)時(shí)間節(jié)點(diǎn),采用神經(jīng)功能缺失評分法評估大鼠神經(jīng)功能缺失癥狀,通過Open field和Elevated plus maze方法評估大鼠手術(shù)后的焦慮水平和自發(fā)運(yùn)動(dòng)能力。此外,通過TTC和CV染色法檢測大鼠腦梗死體積;綜合運(yùn)用Western Blot和免疫熒光技術(shù)研究運(yùn)動(dòng)訓(xùn)練對Agrin表達(dá)時(shí)間窗及細(xì)胞定位的影響,并分析Agrin表達(dá)與突觸再生的關(guān)系。結(jié)果:1、與NE組相比,EE組的大鼠神經(jīng)功能缺失癥狀得到明顯緩解,手術(shù)后的焦慮水平明顯降低,但是對于自發(fā)運(yùn)動(dòng)能力的恢復(fù)沒有顯著的促進(jìn)作用;2、與改善行為學(xué)結(jié)果一致的是,EE組顯著減少缺血大鼠的腦梗死體積、腦水含量;3、Western Blot和Immunofluorescence結(jié)果顯示,早期運(yùn)動(dòng)訓(xùn)練顯著促進(jìn)突觸相關(guān)蛋白表達(dá),上調(diào)神經(jīng)營養(yǎng)因子水平,促進(jìn)突觸再生;4、Agrin可能是運(yùn)動(dòng)訓(xùn)練促進(jìn)突觸再生的重要蛋白。結(jié)論:1、腦卒中后早期運(yùn)動(dòng)訓(xùn)練能夠減少梗死體積,促進(jìn)神經(jīng)功能恢復(fù)。2、腦卒中后早期運(yùn)動(dòng)訓(xùn)練能夠促進(jìn)突觸再生相關(guān)蛋由的表達(dá)。3、腦卒中后早期運(yùn)動(dòng)訓(xùn)練上調(diào)了促突觸再生蛋白Agrin的表達(dá)。
[Abstract]:Aim: to investigate the effect of early exercise training on synaptic regeneration after cerebral ischemia-reperfusion injury and to explore the role of Agrin in promoting synaptic regeneration after cerebral ischemia. Methods: male SD rats were used to construct the left middle cerebral artery occlusion reperfusion (middlecerebral artery occlusion,MCAO) model of 60min. The experiment was divided into three groups: sham operation group (Sham),) without exercise group (non-exercise group,NE), early ischemic exercise group (early exercise group,EE). Sham group) and NE group (rats) were not trained. In EE group, the intensity of exercise training increased 24 hours after MCAO, 30 minutes per day for 14 days. At the corresponding time points after cerebral ischemia-reperfusion injury, neurologic deficit scores were used to evaluate the neurological deficit symptoms in rats, and Open field and Elevated plus maze methods were used to assess the level of anxiety and spontaneous motor ability of rats after operation. In addition, the infarct volume of rat brain was detected by TTC and CV staining, and the effects of exercise training on Agrin expression time window and cell location were studied by Western Blot and immunofluorescence technique, and the relationship between Agrin expression and synaptic regeneration was analyzed. Results: 1.Compared with NE group, the neurological deficit symptoms in EE group were obviously alleviated, and the anxiety level was significantly decreased after operation, but there was no significant effect on the recovery of spontaneous motor ability. 2. In accordance with the results of behavior improvement, EE group significantly reduced the cerebral infarction volume and the content of cerebral water in ischemic rats. The results of Western Blot and Immunofluorescence showed that early exercise training could significantly promote the expression of synaptic related proteins, upregulate the level of neurotrophic factors and promote synaptic regeneration, while Agrin might be an important protein in promoting synaptic regeneration during exercise training. Conclusion: 1. Early exercise training after stroke can reduce the volume of infarction and promote the recovery of neural function. 2. Early exercise training after stroke can promote the expression of synaptic regeneration related egg. Early exercise training after stroke upregulated the expression of synaptic regeneration protein Agrin.
【學(xué)位授予單位】：昆明理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R743.3

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