本文選題：丁�；� + 乙�；���； 參考：《新鄉(xiāng)醫(yī)學(xué)院》2017年碩士論文

【摘要】：背景近年來(lái),全球肥胖的人數(shù)逐年上升,肥胖及其相關(guān)的代謝性疾病已成為影響人類健康的主要疾病之一。脂肪細(xì)胞數(shù)目增加和單個(gè)細(xì)胞體積增大導(dǎo)致脂肪組織增生。蛋白翻譯后修飾(PTMs)引起的分子結(jié)構(gòu)可調(diào)節(jié)細(xì)胞生理活動(dòng)和疾病進(jìn)程,是基因表達(dá)后生物學(xué)研究的主要目標(biāo)之一,有研究表明PTMs會(huì)影響脂肪細(xì)胞分化過(guò)程中的亞細(xì)胞分布,蛋白與蛋白間的相互作用和生物學(xué)活性。近期,一種新的PTM,賴氨酸丁�；揎椩诮M蛋白中被發(fā)現(xiàn),研究顯示丁�；揎椗c精子發(fā)育及某些疾病有著密切聯(lián)系,但至今還沒(méi)有關(guān)于丁�；揎椗c脂肪發(fā)育、脂肪細(xì)胞分化,肥胖以及代謝疾病的相關(guān)報(bào)道。目的探討丁�；揎椩谥炯�(xì)胞分化中的變化,為進(jìn)一步闡明其作用機(jī)制奠定基礎(chǔ)。方法1.動(dòng)物模型的建立:將6周齡C57BL/6品系的小鼠分為兩組,一組給予正常飲食,另一組給予高脂肪飲食,分別在高脂肪飲食的1、5、10周處死小鼠取脂肪組織樣品。2.體重變化趨勢(shì)的檢測(cè):在小鼠高脂肪飲食的第0周起,每隔一周都要稱量體重一次并記錄數(shù)據(jù)。3.Western blot:提取3T3-L1細(xì)胞和小鼠脂肪組織(附睪脂肪)蛋白,分別檢測(cè)丁�；揎椇鸵阴；揎椀谋磉_(dá);4.油紅O染色:染在不同分化時(shí)期的3T3-L1脂肪細(xì)胞,并用顯微鏡觀察其脂滴的表達(dá)量。結(jié)果1.與正常飲食組相比,高脂飲食使小鼠體重和附睪脂肪組織增加更明顯。2.高脂飲食小鼠附睪脂肪組織丁�；揎棻磉_(dá)隨著高脂飲食時(shí)間的延長(zhǎng)而增強(qiáng),乙�；瘺](méi)有這種改變。同時(shí),兩組正常飲食小鼠的這兩種修飾都沒(méi)有發(fā)現(xiàn)改變;3.油紅染色結(jié)果顯示,加入誘導(dǎo)劑Ⅱ的第6天3T3-L1脂肪細(xì)胞始出現(xiàn)脂滴并且隨著誘導(dǎo)時(shí)間增長(zhǎng)脂滴累積增多;4.Western結(jié)果顯示,蛋白丁�；揎椇鸵阴；揎椩�3T3-L1脂肪細(xì)胞分化成熟過(guò)程中逐漸增強(qiáng)。5.用Forskolin刺激成熟脂肪細(xì)胞,與對(duì)照組相比,丁�；揎椇鸵阴；揎楇S著刺激時(shí)間延長(zhǎng)而逐漸減弱。結(jié)論蛋白丁�；揎椏赡苁侵炯�(xì)胞分化和脂肪堆積的必要條件。
[Abstract]:Background in recent years, the number of obese people in the world has increased year by year. Obesity and related metabolic diseases have become one of the major diseases affecting human health. An increase in the number of adipocytes and an increase in the volume of individual cells lead to adipose tissue proliferation. The molecular structure induced by protein post-translational modification (PTMs) regulates cellular physiological activity and disease progression, which is one of the main objectives of post-gene expression biology. Some studies have shown that PTMs affect the distribution of subcells in adipocyte differentiation. Protein-protein interaction and biological activity. Recently, a new type of PTM, lysine butyloyl modification, has been found in histone. Studies have shown that butyloyl modification is closely related to sperm development and some diseases, but there is no study on butyloylation modification and fat development and adipocyte differentiation. Obesity and metabolic diseases are reported. Aim to investigate the changes of butyloyl modification in adipocyte differentiation and to lay a foundation for further elucidation of its mechanism. Method 1. Establishment of animal model: C57BL / 6 strain of 6-week-old mice were divided into two groups: one group was given normal diet and the other group was given high-fat diet. Measurement of body weight change trend: from week 0 of high-fat diet, weight was weighed and data was recorded every other week. 3. Western blot: 3T3-L1 cells and mouse adipose tissue (epididymal fat) protein were extracted. The expression of butyloyl modified and acetylated modified was detected respectively. Oil red O staining: 3T3-L1 adipocytes were stained at different differentiation stages and their lipid droplets were observed by microscope. Result 1. Compared with normal diet group, high fat diet increased body weight and epididymal adipose tissue more significantly. 2. 2. The modified expression of butyloy in epididymal adipose tissue of high fat diet mice increased with the prolongation of high fat diet time, but acetylation did not. At the same time, the two groups of normal diet mice did not find any changes in the two modifications. The results of oil red staining showed that lipid droplets first appeared in 3T3-L1 adipocytes on the 6th day after the addition of inducer 鈪，

本文編號(hào)：2088258