本文選題：原發(fā)性干燥綜合 + 軀體癥狀　； 參考：《北京協(xié)和醫(yī)學院》2015年博士論文

【摘要】：目的評估原發(fā)性干燥綜合征(primary Sjogren's syndrome, pSS)患者軀體癥狀分布情況及外周血NKT (Natural killer T-cells)細胞比例及IFN-y分泌變化；探索NKT細胞在pSS中的調節(jié)機制。方法通過軀體癥狀群量表-15(Patient-Health-Questionnaire-15, PHQ-15)對pSS患者和健康對照者的軀體癥狀發(fā)生及分布情況進行評估,并通過視覺模擬評分(visual analogue scale, VAS)了解患者生活質量受疾病影響程度。使用流式細胞儀檢測pSS患者及健康對照者外周血NKT細胞比例及各亞群變化。使用a-半乳糖神經酰胺體外刺激擴增NKT細胞,評估患者NKT細胞增殖能力。對比血清、細胞培養(yǎng)液上清及NKT細胞胞內IFN-γ分泌變化。結果pSS患者軀體癥狀分布與健康人一致,但發(fā)生率高于健康人,軀體障礙等級也較健康人高。疲勞是pSS患者中最為常見的軀體癥狀。pSS患者外周血NKT細胞比例顯著低于健康人,CD4+CD8-NKT亞群最顯著。體外刺激擴增NKT細胞后IFN-γ分泌水平較健康人增強。結論pSS患者軀體癥狀發(fā)生率顯著高于健康人,軀體障礙程度較健康人嚴重。pSS患者外周血NKT細胞比例降低。pSS患者NKT細胞IFN-γ合成分泌能力增強,Th1/Th2平衡受影響。
[Abstract]:Objective to evaluate the distribution of somatic symptoms in patients with primary Sjogren's syndrome (PSS), the percentage of NKT Natural killer T-cellsand the changes of IFN-y secretion, and to explore the regulatory mechanism of NKT cells in pSS.Methods the occurrence and distribution of somatic symptoms were evaluated by Patient-Health-Questionnaire-15 (PHQ-15) in patients with pSS and healthy controls.The percentage and subsets of NKT cells in peripheral blood of pSS patients and healthy controls were detected by flow cytometry.A-galactose-ceramide was used to stimulate and amplify NKT cells in vitro to evaluate the proliferative ability of NKT cells in patients.The changes of IFN- 緯 secretion in serum, supernatant of cell culture medium and NKT cells were compared.Results the distribution of somatic symptoms in patients with pSS was consistent with that in healthy persons, but the incidence rate was higher than that in healthy persons, and the grade of somatic disorders was higher than that of healthy people.Fatigue is the most common somatic symptom. The percentage of peripheral blood NKT cells in patients with PSS is significantly lower than that in healthy people.The level of IFN- 緯 secretion was increased after stimulation and amplification of NKT cells in vitro.Conclusion the incidence of somatic symptoms in patients with pSS is significantly higher than that in healthy persons. The degree of somatic disorders is significantly lower than that in patients with PSS. The proportion of NKT cells in peripheral blood of patients with PSS is lower. The ability of IFN- 緯 synthesis and secretion of NKT cells in patients with PSS is increased and the balance of Th1 / Th2 is affected.
【學位授予單位】：北京協(xié)和醫(yī)學院
【學位級別】：博士
【學位授予年份】：2015
【分類號】：R593.2

【參考文獻】

相關期刊論文 前4條

1 范華驊,鄭濱,劉[?,高躒,高峰,陸華中;人外周血NKT細胞體外擴增及其分泌細胞因子的研究[J];中國輸血雜志;2004年01期

2 周煒,張奉春,唐福林,董怡;抗SSA噬菌體抗體庫的構建及鑒定[J];中華風濕病學雜志;2003年07期

3 李婭杰,劉莉,張奉春;SSA抗原及其不同陽性表位的臨床意義[J];中華內科雜志;2003年03期

4 李子濤;楊濱燕;吳長有;;人外周血NKT細胞亞群表型及生物學特征[J];中國免疫學雜志;2011年02期

，

本文編號：1734441