【摘要】：目的：觀(guān)察樹(shù)突狀細(xì)胞（DC）聯(lián)合細(xì)胞因子誘導(dǎo)的殺傷細(xì)胞（CIK）免疫治療對(duì)腎癌術(shù)后患者免疫功能的影響。 方法：回顧性分析2011年9月至2013年6月期間在我院確診的52例腎癌患者，均采取腎癌根治性切除手術(shù)或保留腎單位手術(shù)，術(shù)后隨訪(fǎng)過(guò)程中曾予以行DC聯(lián)合CIK細(xì)胞輔助免疫治療，分別于治療前一周、治療后兩周，抽取患者外周血采用流式細(xì)胞術(shù)和酶聯(lián)免疫吸附法檢測(cè)患者免疫功能指標(biāo)，包括外周血CD3+、CD4+、CD8+T淋巴細(xì)胞亞群百分比、CD4+/CD8+的比值，以及外周血INF-、IL-2、IL-10等細(xì)胞因子水平；同時(shí)記錄回輸過(guò)程中的毒副反應(yīng)，以及評(píng)估患者的生活質(zhì)量。 結(jié)果：1.治療后兩周外周血CD3+、CD4+T淋巴細(xì)胞百分比、CD4+/CD8+的比值、IL-2、INF-水平較治療前一周升高，2.治療后兩周外周血CD8+T淋巴細(xì)胞百分比、IL-10水平較治療前一周下降，前后兩者比較差異均有統(tǒng)計(jì)學(xué)意義(淋巴細(xì)胞比例P0.05，細(xì)胞因子P0.01)；3.治療過(guò)程中，毒副反應(yīng)較少，患者的生活質(zhì)量較前明顯改善。 結(jié)論：DC聯(lián)合CIK細(xì)胞免疫治療能顯著改善腎癌術(shù)后患者的免疫失衡狀態(tài)，激發(fā)特異性免疫應(yīng)答，提高患者的免疫功能；患者不良反應(yīng)較少，耐受性良好；可能降低腎癌術(shù)后腫瘤的復(fù)發(fā)率，并且對(duì)于轉(zhuǎn)移性腎癌的治療也顯現(xiàn)一定的療效。
[Abstract]:Aim: to observe the effect of dendritic cell (DC) combined with cytokine induced killer cell (CIK) immunotherapy on immune function of patients with renal cell carcinoma after operation. Methods: from September 2011 to June 2013, 52 patients with renal cell carcinoma diagnosed in our hospital were treated with radical resection of renal cell carcinoma or nephron-sparing surgery. DC combined with CIK cell adjuvant immunotherapy was performed during follow-up. Flow cytometry and enzyme-linked immunosorbent assay (Elisa) were used to detect the immune function, including the percentage of CD3, CD4, CD8 T lymphocyte subsets and the ratio of CD4 / CD8. And the level of cytokines such as INF-,IL-2,IL-10 in peripheral blood; Side effects were also recorded during retransfusion and quality of life was assessed. Results: 1. Two weeks after treatment, the percentage of CD3, CD4 T lymphocytes, the ratio of CD4 / CD8, and the level of IL-2,INF- in peripheral blood were higher than those in the week before treatment. Two weeks after treatment, the percentage of CD8 T lymphocytes and the level of IL-10 in peripheral blood were significantly lower than those before treatment (P 0.05, cytokine P0.01). In the course of treatment, the side effects were less and the quality of life was improved obviously. Conclusion: DC combined with CIK cell immunotherapy can significantly improve the immune imbalance, stimulate specific immune response and improve the immune function of patients with renal cell carcinoma, with less adverse reactions and good tolerance. It may reduce the recurrence rate of renal cell carcinoma after operation, and also show certain curative effect for metastatic renal cell carcinoma.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R737.11

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