蒺藜提取物提高大強(qiáng)度耐力運(yùn)動(dòng)大鼠的運(yùn)動(dòng)能力及其機(jī)制
發(fā)布時(shí)間：2019-06-17 18:58
【摘要】：1.目的 研究蒺藜提取物對(duì)大強(qiáng)度耐力運(yùn)動(dòng)大鼠運(yùn)動(dòng)能力的影響及其機(jī)制。 2.方法 32只SD大鼠隨機(jī)分為4組：安靜對(duì)照組（C，n=8）、蒺藜組（TT，n=8）、運(yùn)動(dòng)組（E，n=8）和蒺藜+運(yùn)動(dòng)組（TT+E，n=8）。安靜對(duì)照組和運(yùn)動(dòng)組大鼠自由飲用純水，而蒺藜組和蒺藜+運(yùn)動(dòng)組大鼠自由飲用蒺藜提取物配成的蒺藜飲料，記錄每日大鼠的飲用量（大鼠攝取的蒺藜提取物在120mg/kg體重左右）。運(yùn)動(dòng)大鼠進(jìn)行大強(qiáng)度的五周遞增負(fù)荷運(yùn)動(dòng)，每周運(yùn)動(dòng)6天。5周耐力運(yùn)動(dòng)的倒數(shù)第二天進(jìn)行30min的強(qiáng)度為30m/min的跑臺(tái)運(yùn)動(dòng)，斷尾取血，檢測(cè)運(yùn)動(dòng)前和運(yùn)動(dòng)后30min、2h的血糖水平。5周耐力運(yùn)動(dòng)的最后一天，各組大鼠進(jìn)行一次力竭運(yùn)動(dòng)。力竭運(yùn)動(dòng)結(jié)束后36h，大鼠麻醉后下腔靜脈取血，檢測(cè)血漿睪酮(Testosterone，T)水平、胰島素樣生長(zhǎng)因子1（Insulin-like growth factor1，IGF-1）水平、血紅細(xì)胞數(shù)（RBC）、血紅蛋白含量（Hb）。大鼠處死后取腓腸肌、比目魚(yú)肌，秤重，用BCA試劑盒檢測(cè)總蛋白含量；用western blot方法檢測(cè)雄激素受體（Androgenreceptor，AR）、胰島素樣生長(zhǎng)因子1受體（Insulin-like growth factor receptor，IGF-1R）和肌球蛋白重鏈（Mysion heavy chain，MHC）的蛋白水平。 3.結(jié)果 1）蒺藜提取物提高大強(qiáng)度耐力運(yùn)動(dòng)大鼠的運(yùn)動(dòng)能力 （1）蒺藜提取物減輕大強(qiáng)度耐力運(yùn)動(dòng)大鼠體重降低的程度 實(shí)驗(yàn)前各組大鼠體重?zé)o顯著性差異。從第三周開(kāi)始，運(yùn)動(dòng)組、蒺藜+運(yùn)動(dòng)組大鼠的體重顯著低于安靜對(duì)照組（p0.05），該作用一直持續(xù)至實(shí)驗(yàn)結(jié)束（p0.01）。與運(yùn)動(dòng)組比較，蒺藜+運(yùn)動(dòng)組大鼠的體重在第五周時(shí)顯著增加（p0.05）。此外，蒺藜組大鼠的體重與安靜對(duì)照組相比，在各周都無(wú)顯著性差異。 （2）蒺藜提取物提高大強(qiáng)度耐力運(yùn)動(dòng)大鼠的一次力竭運(yùn)動(dòng)時(shí)間 與對(duì)照組相比，蒺藜組大鼠的一次力竭運(yùn)動(dòng)時(shí)間無(wú)顯著差異。五周耐力運(yùn)動(dòng)后，蒺藜+運(yùn)動(dòng)組大鼠的一次力竭運(yùn)動(dòng)時(shí)間比運(yùn)動(dòng)組大鼠顯著延長(zhǎng)（p0.01），約延長(zhǎng)了63.2%。此外，運(yùn)動(dòng)訓(xùn)練大鼠的一次力竭運(yùn)動(dòng)時(shí)間比對(duì)照組顯著延長(zhǎng)（p0.05）。 2）蒺藜提取物提高大強(qiáng)度耐力運(yùn)動(dòng)大鼠運(yùn)動(dòng)能力的機(jī)制 （1）蒺藜提取物提高大強(qiáng)度耐力運(yùn)動(dòng)大鼠的血漿睪酮水平和腓腸肌AR的蛋白水平 與安靜對(duì)照組相比，運(yùn)動(dòng)組大鼠的血漿睪酮水平顯著降低（p0.01）；蒺藜+運(yùn)動(dòng)組大鼠的睪酮水平與運(yùn)動(dòng)組比較顯著增加（p0.01），與對(duì)照組相比無(wú)顯著差異，表明蒺藜提取物能完全逆轉(zhuǎn)大強(qiáng)度運(yùn)動(dòng)所造成的血睪酮水平降低。此外，與安靜組相比較，運(yùn)動(dòng)組大鼠腓腸肌的AR蛋白含量無(wú)顯著性差異。蒺藜+運(yùn)動(dòng)組大鼠腓腸肌的AR蛋白表達(dá)水平比運(yùn)動(dòng)組的顯著升高（p0.05）。 （2）蒺藜提取物提高大強(qiáng)度耐力運(yùn)動(dòng)大鼠的血漿IGF-1水平和腓腸肌IGF-1R的蛋白水平 與安靜對(duì)照組相比，運(yùn)動(dòng)組大鼠血漿的IGF-1的水平?jīng)]有顯著性差異。蒺藜+運(yùn)動(dòng)組大鼠的IGF-1水平與運(yùn)動(dòng)組相比較顯著增加（p0.05），表明補(bǔ)充蒺藜提取物能提高大強(qiáng)度運(yùn)動(dòng)大鼠血漿的IGF-1水平。此外，運(yùn)動(dòng)組與安靜組相比較，腓腸肌IGF-1R蛋白含量沒(méi)有顯著性差異，蒺藜+運(yùn)動(dòng)組與運(yùn)動(dòng)組相比較，腓腸肌IGF-1R蛋白表達(dá)水平顯著升高（p0.05）。 （3）蒺藜提取物增加大強(qiáng)度耐力運(yùn)動(dòng)大鼠腓腸肌的總蛋白濃度和MHC的蛋白水平 與對(duì)照組相比，運(yùn)動(dòng)組大鼠比目魚(yú)肌和腓腸肌的相對(duì)重量均顯著增加、且腓腸肌的蛋白濃度也顯著增加；而蒺藜組大鼠的比目魚(yú)肌和腓腸肌的相對(duì)重量、蛋白濃度均無(wú)顯著差異。與運(yùn)動(dòng)組比較，蒺藜+運(yùn)動(dòng)組大鼠腓腸肌的蛋白濃度顯著增加、但腓腸肌的相對(duì)重量以及比目魚(yú)肌的蛋白濃度都沒(méi)有顯著性差異，而比目魚(yú)肌相對(duì)重量卻比較意外地下降（p0.05）。此外，與安靜組相比，運(yùn)動(dòng)組的MHC蛋白水平?jīng)]有顯著性差異。與運(yùn)動(dòng)組相比，蒺藜+運(yùn)動(dòng)組的MHC蛋白水平顯著增高（p0.05）。 （4）藜提取物增加大強(qiáng)度耐力運(yùn)動(dòng)和不運(yùn)動(dòng)大鼠的一次力竭運(yùn)動(dòng)后30min的血糖水平 蒺藜提取物能降低運(yùn)動(dòng)和不運(yùn)動(dòng)大鼠運(yùn)動(dòng)前的血糖水平。蒺藜組與安靜組比較、蒺藜+運(yùn)動(dòng)組與運(yùn)動(dòng)組比較，血糖水平在一次力竭運(yùn)動(dòng)后的30min顯著增加（分別是p0.05，p0.01），但在運(yùn)動(dòng)后2h，各組血糖水平無(wú)顯著差異。 （5）蒺藜提取物對(duì)大強(qiáng)度耐力運(yùn)動(dòng)和不運(yùn)動(dòng)大鼠的Hb、紅細(xì)胞數(shù)無(wú)影響 4.結(jié)論： 1）蒺藜提取物延長(zhǎng)大強(qiáng)度耐力運(yùn)動(dòng)大鼠的一次力竭運(yùn)動(dòng)時(shí)間，提高其運(yùn)動(dòng)能力。 2）蒺藜提取物增強(qiáng)大強(qiáng)度耐力運(yùn)動(dòng)大鼠運(yùn)動(dòng)能力的機(jī)制可能與其增加睪酮/AR水平和IGF-1/IGF-1R水平，，從而使腓腸肌的蛋白濃度、MHC蛋白水平增加，以及增加一次力竭運(yùn)動(dòng)后30min的血糖水平有關(guān)。 3）蒺藜提取物增強(qiáng)大強(qiáng)度耐力運(yùn)動(dòng)大鼠運(yùn)動(dòng)能力的機(jī)制與Hb和RBC數(shù)無(wú)關(guān)。
[Abstract]:1. Purpose The effect of the extract of the extract on the exercise ability of the large-strength endurance exercise rats and its machine System. 2. Methods 32 SD rats were randomly divided into 4 groups: the quiet control group (C, n = 8), the control group (TT, n = 8), the exercise group (E, n = 8), and the Astragalus + exercise group (TT + E). , n = 8). The quiet control group and the exercise group rats were free to drink pure water, while the rats in the control group and the exercise group were free to drink pure water, and the drinking quantity of the daily rats (the rat's uptake of the ginkgo leaf extract at 120 mg/ kg) was recorded. body weight). The exercise rats were subjected to five-week incremental load exercise with a large intensity, and the exercise was carried out for 6 days a week. The last day of the 5-week endurance exercise was performed with a treadmill exercise of 30 m/ min for 30 minutes, the blood glucose level of 2 hours was measured at 30 min and 2 hours before and after the exercise, and the 5-week endurance exercise One day of the last day, each group of rats The level of serum testosterone (T), insulin-like growth factor 1 (IGF-1), blood red blood cell count (RBC) and hemoglobin were measured at 36 h after the end of exhaustive exercise. (Hb). After the rats were sacrificed, the gastrocnemius muscle, the flounder muscle and the weight were weighed, and the total protein content was detected by the BCA kit; the androgen receptor (AR), the insulin-like growth factor receptor (IGF-1R) and the myosin heavy chain (MHC) were detected by western blot. protein water Ping.3. Results 1) The extract of the extract of the extract of the invention has the advantages of high strength, The exercise ability (1) of the force-moving rats to reduce the high-strength resistance to the extract Experimental study on the degree of weight loss in rats with force exercise There was no significant difference in the weight of the rats in the first group. From the third week, the weight of the rats in the exercise group and the group of the rats of the positive group was significantly lower than that of the quiet control group (p0.05), and the weight of the rats was always the same as that of the control group (p0.05). continued until the end of the experiment (p0.01). In comparison with the exercise group, the body weight of the baboon + exercise group was at the fifth Significant increases at week (p0.05). In addition, the body weight and the quiet control group in the treatment group There was no significant difference in each week. The time of exhaustive exercise of endurance sports rats was compared with that of the control group. There was no significant difference in the time of exhaustive exercise of the rats in the group. After the five-week endurance exercise, the time of exhaustive exercise of the rats in the group of exercise group and exercise group was significantly longer than that of the rats in the exercise group. (p0.01), about 63.2%. In addition, the exercise training rats' exhaustive exercise The time was significantly longer than that in the control group (p0.05). A mechanism (1) to improve the exercise capacity of a large-strength endurance exercise rat by taking the object (1) to improve the strength and endurance of the extract Plasma Testosterone in Exercise Rats Compared with the control group, the level of serum testosterone and the level of serum testosterone in the control group were significantly lower than that of the control group (p0.01), and the level of testosterone in the rats of the group of the rats of the group of the group of the rats of the group of the group of the group of the group of the group of the group of the group of the control group was significantly higher than that of the control group (p0.01), and there was no significant difference in the level of testosterone and the control group, indicating that the level of the serum testosterone of the rats in the exercise group The product can completely reverse the level of blood testosterone caused by the large-intensity exercise. In addition, compared with the quiet group, There was no significant difference in the AR protein content of the gastrocnemius in the exercise group. The expression of R-protein was significantly higher than that of the exercise group (p0.05). (2) The extract of Astragalus extract increased the exercise of large-intensity endurance. The level of IGF-1 and the level of IGF-1R in the gastrocnemius of the rat and the quiet control There was no significant difference in the level of IGF-1 in the rats in the exercise group compared with the group. In addition, the content of IGF-1R in the gastrocnemius was not significantly different from that of the quiet group, and the level of IGF-1R in the control group was compared with that of the exercise group. The expression of IGF-1R in gastrocnemius was significantly higher (p0.05). (3) The expression of IGF-1R in gastrocnemius was significantly higher (p0.05). (3) The expression of IGF-1R in gastrocnem@@ Compared with the control group, the total protein concentration of the gastrocnemius muscle and the protein level of MHC in the rats with high-intensity endurance exercise were higher than that of the control group. The relative weight of the fish muscle and the gastrocnemius was significantly increased, and the protein concentration of the gastrocnemius was also increased. The relative weight and protein concentration of the soleus muscle and the gastrocnemius muscle in the rats were not significantly different from those in the exercise group, but the relative weight of the gastrocnemius and the protein concentration of the soleus muscle were not increased. There was a significant difference, but the relative weight of the soleus was reduced unexpectedly (p0.05). 5). In addition, there was no significant difference in the level of MHC protein in the exercise group compared to the quiet group. Compared with the exercise group, the level of MHC protein was significantly higher in the + exercise group (p0.05). (4) The level of MHC protein in the exercise group was significantly higher than that of the exercise group (p0.05). After one-time exhaustive exercise of the high-intensity endurance exercise and the non-moving rats The blood glucose level of 0 min can decrease the blood glucose level before the exercise and the non-moving rats. The blood glucose level is compared with the quiet group, and the blood sugar level is significantly increased at 30 min after the exhaustive exercise. The blood glucose levels in each group were not significant after 2 h after exercise, respectively (p0.05, p0.01). The difference. (5) The amount of Hb and red blood cells in the high-strength endurance exercise and the non-moving rats were not affected by the extract of the extract. Conclusion:1) The mechanism of enhancing the exercise ability of the rats with high-intensity endurance exercise may increase the level of testosterone/ AR and the level of IGF-1/ IGF-1R. the protein concentration of the gastrocnemius muscle, the level of the MHC protein,
【學(xué)位授予單位】：上海體育學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：G804.2
本文編號(hào)：2501190
[Abstract]:1. Purpose The effect of the extract of the extract on the exercise ability of the large-strength endurance exercise rats and its machine System. 2. Methods 32 SD rats were randomly divided into 4 groups: the quiet control group (C, n = 8), the control group (TT, n = 8), the exercise group (E, n = 8), and the Astragalus + exercise group (TT + E). , n = 8). The quiet control group and the exercise group rats were free to drink pure water, while the rats in the control group and the exercise group were free to drink pure water, and the drinking quantity of the daily rats (the rat's uptake of the ginkgo leaf extract at 120 mg/ kg) was recorded. body weight). The exercise rats were subjected to five-week incremental load exercise with a large intensity, and the exercise was carried out for 6 days a week. The last day of the 5-week endurance exercise was performed with a treadmill exercise of 30 m/ min for 30 minutes, the blood glucose level of 2 hours was measured at 30 min and 2 hours before and after the exercise, and the 5-week endurance exercise One day of the last day, each group of rats The level of serum testosterone (T), insulin-like growth factor 1 (IGF-1), blood red blood cell count (RBC) and hemoglobin were measured at 36 h after the end of exhaustive exercise. (Hb). After the rats were sacrificed, the gastrocnemius muscle, the flounder muscle and the weight were weighed, and the total protein content was detected by the BCA kit; the androgen receptor (AR), the insulin-like growth factor receptor (IGF-1R) and the myosin heavy chain (MHC) were detected by western blot. protein water Ping.3. Results 1) The extract of the extract of the extract of the invention has the advantages of high strength, The exercise ability (1) of the force-moving rats to reduce the high-strength resistance to the extract Experimental study on the degree of weight loss in rats with force exercise There was no significant difference in the weight of the rats in the first group. From the third week, the weight of the rats in the exercise group and the group of the rats of the positive group was significantly lower than that of the quiet control group (p0.05), and the weight of the rats was always the same as that of the control group (p0.05). continued until the end of the experiment (p0.01). In comparison with the exercise group, the body weight of the baboon + exercise group was at the fifth Significant increases at week (p0.05). In addition, the body weight and the quiet control group in the treatment group There was no significant difference in each week. The time of exhaustive exercise of endurance sports rats was compared with that of the control group. There was no significant difference in the time of exhaustive exercise of the rats in the group. After the five-week endurance exercise, the time of exhaustive exercise of the rats in the group of exercise group and exercise group was significantly longer than that of the rats in the exercise group. (p0.01), about 63.2%. In addition, the exercise training rats' exhaustive exercise The time was significantly longer than that in the control group (p0.05). A mechanism (1) to improve the exercise capacity of a large-strength endurance exercise rat by taking the object (1) to improve the strength and endurance of the extract Plasma Testosterone in Exercise Rats Compared with the control group, the level of serum testosterone and the level of serum testosterone in the control group were significantly lower than that of the control group (p0.01), and the level of testosterone in the rats of the group of the rats of the group of the group of the rats of the group of the group of the group of the group of the group of the group of the group of the control group was significantly higher than that of the control group (p0.01), and there was no significant difference in the level of testosterone and the control group, indicating that the level of the serum testosterone of the rats in the exercise group The product can completely reverse the level of blood testosterone caused by the large-intensity exercise. In addition, compared with the quiet group, There was no significant difference in the AR protein content of the gastrocnemius in the exercise group. The expression of R-protein was significantly higher than that of the exercise group (p0.05). (2) The extract of Astragalus extract increased the exercise of large-intensity endurance. The level of IGF-1 and the level of IGF-1R in the gastrocnemius of the rat and the quiet control There was no significant difference in the level of IGF-1 in the rats in the exercise group compared with the group. In addition, the content of IGF-1R in the gastrocnemius was not significantly different from that of the quiet group, and the level of IGF-1R in the control group was compared with that of the exercise group. The expression of IGF-1R in gastrocnemius was significantly higher (p0.05). (3) The expression of IGF-1R in gastrocnemius was significantly higher (p0.05). (3) The expression of IGF-1R in gastrocnem@@ Compared with the control group, the total protein concentration of the gastrocnemius muscle and the protein level of MHC in the rats with high-intensity endurance exercise were higher than that of the control group. The relative weight of the fish muscle and the gastrocnemius was significantly increased, and the protein concentration of the gastrocnemius was also increased. The relative weight and protein concentration of the soleus muscle and the gastrocnemius muscle in the rats were not significantly different from those in the exercise group, but the relative weight of the gastrocnemius and the protein concentration of the soleus muscle were not increased. There was a significant difference, but the relative weight of the soleus was reduced unexpectedly (p0.05). 5). In addition, there was no significant difference in the level of MHC protein in the exercise group compared to the quiet group. Compared with the exercise group, the level of MHC protein was significantly higher in the + exercise group (p0.05). (4) The level of MHC protein in the exercise group was significantly higher than that of the exercise group (p0.05). After one-time exhaustive exercise of the high-intensity endurance exercise and the non-moving rats The blood glucose level of 0 min can decrease the blood glucose level before the exercise and the non-moving rats. The blood glucose level is compared with the quiet group, and the blood sugar level is significantly increased at 30 min after the exhaustive exercise. The blood glucose levels in each group were not significant after 2 h after exercise, respectively (p0.05, p0.01). The difference. (5) The amount of Hb and red blood cells in the high-strength endurance exercise and the non-moving rats were not affected by the extract of the extract. Conclusion:1) The mechanism of enhancing the exercise ability of the rats with high-intensity endurance exercise may increase the level of testosterone/ AR and the level of IGF-1/ IGF-1R. the protein concentration of the gastrocnemius muscle, the level of the MHC protein,
【學(xué)位授予單位】：上海體育學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：G804.2
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 王啟榮;喬莉;周麗麗;高紅;許葆華;楊則宜;;耐力訓(xùn)練與中藥干預(yù)對(duì)大鼠骨骼肌雄激素受體表達(dá)的影響[J];北京體育大學(xué)學(xué)報(bào);2009年12期
2 郝鑫鑫;曾凡星;趙華;歐陽(yáng)芡;;運(yùn)動(dòng)與注射IGF-1對(duì)大鼠骨骼肌mTOR、AR蛋白表達(dá)的影響[J];北京體育大學(xué)學(xué)報(bào);2010年05期
3 蘇衛(wèi)東;徐雅娟;;蒺藜的研究進(jìn)展[J];長(zhǎng)春中醫(yī)藥大學(xué)學(xué)報(bào);2006年03期
4 孫梅;;淺談促合成代謝類固醇激素對(duì)人體健康的影響[J];河南科技學(xué)院學(xué)報(bào)(自然科學(xué)版);2006年04期
5 朱辛為;李質(zhì)馨;陳為;;蒺藜皂苷抗衰老作用的研究進(jìn)展[J];吉林醫(yī)藥學(xué)院學(xué)報(bào);2010年02期
6 姜濤 ,姜輝 ,劉用楫;前列腺癌雄激素受體研究進(jìn)展[J];中國(guó)男科學(xué)雜志;2005年02期
7 王齊敏;賀巖;李瑩杰;金曉明;;MHC與HSP70在胃癌組織中表達(dá)的意義[J];實(shí)用腫瘤學(xué)雜志;2006年05期
8 葉鳴;賀道遠(yuǎn);劉霞;曾凡星;王煜;;低氧和運(yùn)動(dòng)經(jīng)AR-IGF-1對(duì)骨骼肌蛋白質(zhì)合成的作用[J];天津體育學(xué)院學(xué)報(bào);2010年02期
9 許豪文;補(bǔ)充糖對(duì)運(yùn)動(dòng)員訓(xùn)練和競(jìng)技能力的影響[J];體育科研;1996年02期
10 鄭陸,潘力平,陶大浪,沈東穎;睪酮、骨骼肌雄激素受體與運(yùn)動(dòng)的關(guān)系[J];武漢體育學(xué)院學(xué)報(bào);2004年02期
本文編號(hào)：2501190
本文鏈接：http://www.sikaile.net/yixuelunwen/mazuiyixuelunwen/2501190.html
最近更新
教材專著
