本文關(guān)鍵詞： 控制性低血壓 腦紅蛋白 大鼠　出處：《廣東醫(yī)學(xué)》2017年S2期 　論文類型：期刊論文

【摘要】：目的觀察控制性低血壓持續(xù)2 h對大鼠血漿Ngb表達(dá)的影響。方法 30只SD大鼠隨機(jī)分為手術(shù)對照組(A組)和控制性MAP 60 mm Hg持續(xù)2 h組(C組)。C組按控制性低血壓后恢復(fù)時(shí)間的不同分為4組,C1組為控制性低血壓后6 h;C2組為控制性低血壓后12 h;C3組為控制性低血壓后24 h;C4組為控制性低血壓后48 h。C組用硝普鈉復(fù)合鹽酸艾司洛爾誘導(dǎo)控制性低血壓。在術(shù)中使用機(jī)械通氣技術(shù)保證術(shù)中氧供。酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA)法檢測血漿Ngb的表達(dá)。結(jié)果 (1)術(shù)中大鼠的肛溫維持在37~38℃。術(shù)中均能夠通過血管活性藥物泵速的調(diào)節(jié)和麻醉深度的調(diào)節(jié)維持MAP在57~63 mm Hg的范圍內(nèi)波動,各小組內(nèi)和各小組間的變化不明顯;在停止使用血管活性藥物后所有動物的MAP均能逐漸回升。所有動物在復(fù)壓階段均沒有使用升壓藥物。所有實(shí)驗(yàn)動物在術(shù)中,生命體征平穩(wěn)。各時(shí)間點(diǎn)存活率100%。硝普鈉復(fù)合鹽酸艾司洛爾誘導(dǎo)控制性低血壓后,大鼠C組大鼠血漿中Ngb的表達(dá)逐漸增強(qiáng),至24 h達(dá)峰,其后逐漸下降。結(jié)論在硝普鈉復(fù)合鹽酸艾司洛爾誘導(dǎo)控制性低血壓后,控制性低血壓的相關(guān)神經(jīng)損傷風(fēng)險(xiǎn)在術(shù)后24 h后逐漸增強(qiáng)。
[Abstract]:Objective to observe the effect of controlled hypotension on plasma Ngb expression in rats for 2 h. Methods Thirty SD rats were randomly divided into two groups: control group A (n = 30) and controlled MAP 60 mm Hg group (n = 2). The recovery time was divided into 4 groups: control hypotension group C 1, control hypotension group C 2, control hypotension 12 h, control C 3, control hypotension 24 h, C 4, 48 h after controlled hypotension, group C with sodium nitroprusside combined with esmolol hydrochloride. Inducible hypotension. Oxygen supply was ensured by mechanical ventilation during operation. Plasma Ngb expression was detected by Elisa. Results 1) the anus temperature of rats during operation was maintained at 37 ~ 38 鈩，

本文編號：1544611