本文選題：牙周膜干細胞 + 年齡因素��； 參考：《南方醫(yī)科大學學報》2017年03期

【摘要】：目的評估年齡因素對牙周膜干細胞(PDLSC)增殖、分化能力的影響。方法選取來源于12位不同的患者因阻生需要拔除的第三磨牙12顆,根據(jù)患者年齡分為A組(年齡18~20歲)、B組(年齡45~50歲)。分離培養(yǎng)獲得PDLSC。MTT檢測A、B組PDLSC增殖能力;進行成骨、成脂誘導,并用茜素紅染色及油紅O染色評價其分化能力;SA-βG染色分析A、B組細胞衰老情況;將A、B組PDLSC成骨誘導7 d后進行ALP染色并同時采用Western blotting檢測OCN、ALP的表達。結果不同年齡階段的供體中都能分離出PDLSC,然而其增殖和成骨分化能力隨年齡增加而降低,成脂分化能力和SA-βG表達隨年齡增加而增加。ALP和OCN成骨誘導7 d后表達水平均升高,且A組高于B組。結論隨年齡增長能在牙周膜中獲取PDLSC,隨年齡增加其增殖與成骨分化能力下降,但成脂分化能力和SA-β表達則增加。
[Abstract]:Objective to evaluate the effect of age on the proliferation and differentiation of periodontal ligament stem cells (PDLSCs). Methods Twelve third molars from 12 patients were divided into group A (n = 18, 20 years old) and B group (n = 45 ~ 50 years old) according to the age of the patients. PDLSC.MTT assay was used to detect the proliferation of PDLSC, osteogenesis and lipogenesis were performed. The differentiation ability of PDLSC was evaluated by alizarin red staining and oil red O staining. ALP staining was performed 7 days after osteogenesis induction of PDLSC in Agna B group and the expression of OCN-ALP was detected by Western blotting. Results PDLSCs could be isolated from donors at different age stages. However, the proliferation and osteogenic differentiation ability of PDLSCs decreased with age, and the expression of SA- 尾 G and adipogenic differentiation increased with age. ALP and OCN increased after osteogenic induction for 7 days. Group A was higher than group B. Conclusion PDLSCs can be obtained from periodontal ligament with age, but the ability of adipogenic differentiation and SA- 尾 expression increase with age.
【作者單位】： 中國人民解放軍總醫(yī)院口腔科;中國科學院理化技術研究所;
【基金】：國家自然科學基金(31670998,51473175) 北京市科技新星計劃(Z14144000180000-2014101) 中國博士后科學基金特別資助項目(2014T70959)~~
【分類號】：R781.4

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本文編號：2038995