【摘要】：目的：利用事件相關(guān)電位技術(shù)，使用2-back范式，在不同的睪酮水平下對字母匹配任務(wù)和空間匹配任務(wù)進(jìn)行測試，以觀察工作記憶的行為學(xué)和腦電波記錄的結(jié)果，分析不同的睪酮水平下不同工作記憶任務(wù)的差異，同時(shí)也反映出睪酮對腦功能的影響。 方法：本研究從大連醫(yī)科大學(xué)2011年445名新入學(xué)男性中隨機(jī)抽取100名進(jìn)行血清睪酮水平測驗(yàn)，100名男生被試均為正常健康人。然后根據(jù)100名被試者血清睪酮水平按四分位法分為四個(gè)組，然后將最高睪酮水平組25人和最低睪酮水平組25人為研究對象，在征詢被試同意后，排除無內(nèi)分泌生殖疾病史、無精神疾病史、無外傷史和無其他身體疾病史者，年齡限制在17-19歲，均為右利手，智力水平無顯著差異。最后選出12人入組高血清睪酮水平組，12人入組低血清睪酮水平組。刺激由E-prime1.0軟件呈現(xiàn)，實(shí)驗(yàn)分為兩個(gè)任務(wù)。第一個(gè)任務(wù)刺激內(nèi)容為字母，第二個(gè)任務(wù)為空間位置的判斷。兩種類型的刺激任務(wù)分別都有150個(gè)刺激序列(Trial)，其中目標(biāo)刺激和非目標(biāo)刺激等概率隨機(jī)出現(xiàn)（各50%），在非目標(biāo)刺激中還有15個(gè)干擾刺激出現(xiàn)（占10%）。完成一個(gè)任務(wù)大約需10分鐘，中間休息3分鐘，完成整個(gè)實(shí)驗(yàn)大約需要30分鐘左右。行為學(xué)結(jié)果反應(yīng)時(shí)、命中率、誤報(bào)率和干擾刺激反應(yīng)時(shí)使用獨(dú)立樣本t檢驗(yàn)方法進(jìn)行兩組之間比較，對事件相關(guān)電位成分采用重復(fù)測量的方差分析，并進(jìn)一步進(jìn)行事后分析。 結(jié)果： （1）行為學(xué)結(jié)果中，高低睪酮組之間的字母匹配任務(wù)刺激反應(yīng)時(shí)、命中率、誤報(bào)率和干擾刺激反應(yīng)時(shí)結(jié)果差別均不具有統(tǒng)計(jì)學(xué)意義，而刺激反應(yīng)時(shí)和命中率方面差異具有統(tǒng)計(jì)學(xué)傾向性。高低睪酮組之間的空間匹配任務(wù)刺激結(jié)果發(fā)現(xiàn)命中率在高睪酮組顯著高于低睪酮組，而在反應(yīng)時(shí)、誤報(bào)率和干擾刺激反應(yīng)時(shí)差別均不具有統(tǒng)計(jì)學(xué)意義。 （2）波形圖中，在字母匹配刺激任務(wù)下，血清高睪酮水平組所有的位點(diǎn)都沒有發(fā)現(xiàn)左中右激活的差異；而低血清睪酮水平組左中右間的激活差異較明顯，主要表現(xiàn)在額區(qū)、中央?yún)^(qū)和頂區(qū)（F4,C4,P4,PZ）。在空間匹配任務(wù)下，高血清睪酮水平組在中央額區(qū)激活的波幅明顯大于左右側(cè)，在中央頂區(qū)激活的波幅明顯比左右側(cè)頂區(qū)大；低血清睪酮水平組在右側(cè)額區(qū)的激活比左側(cè)額區(qū)F3和中央額區(qū)FZ都明顯；中央頂區(qū)的激活較兩側(cè)要明顯。在其他位置波幅的差異不明顯。 （3）空間和字母匹配任務(wù)在280-400ms之間誘發(fā)的平均腦電地形圖中，空間匹配任務(wù)血清睪酮水平高低兩組的差異比較明顯，而字母匹配任務(wù)的差異不是很明顯，在空間匹配任務(wù)中高血清睪酮水平組的激活更明顯一些，而在字母匹配任務(wù)中低血清睪酮水平組的激活則要相對較強(qiáng)。另外在兩個(gè)匹配任務(wù)中血清低睪酮水平組的激活區(qū)域向右側(cè)半球傾斜，而這種傾向在字母匹配任務(wù)中體現(xiàn)的更為明顯，而高血清睪酮水平組的激活區(qū)域則主要集中在中央?yún)^(qū)域，向兩側(cè)傾斜的趨勢不很明顯。在圖中還可以看出激活最強(qiáng)的區(qū)域主要集中在頂區(qū)，而中央?yún)^(qū)和前額葉的激活比較弱。 （4）對空間和字母匹配任務(wù)在280-400ms之間誘發(fā)的平均腦電峰值進(jìn)行統(tǒng)計(jì)分析，在字母匹配任務(wù)中，腦葉的主效應(yīng)顯著，腦區(qū)和組別的主效應(yīng)不顯著，腦區(qū)和組別的交互效應(yīng)顯著其他比較結(jié)果不顯著。在空間匹配任務(wù)中，，腦葉主效應(yīng)顯著，腦區(qū)和組別的主效應(yīng)不顯著，腦區(qū)和組別以及腦葉、腦區(qū)和組別的交互效應(yīng)都很顯著，其他統(tǒng)計(jì)結(jié)果不顯著。 結(jié)論： 1.字母匹配任務(wù)，低睪酮水平組呈現(xiàn)了右側(cè)半球代償性優(yōu)勢激活狀態(tài)，而高睪酮水平組，左右同時(shí)加工，差異不顯著； 2.空間匹配任務(wù)，高睪酮水平組揭示了更高的加工狀態(tài)，特別是中央?yún)^(qū)和頂區(qū)。
[Abstract]:AIM: To observe the behavior of working memory and the results of electroencephalogram (EEG) recording, analyze the differences of different working memory tasks at different levels of testosterone, and also reflect the effects of testosterone on brain function. Influence of energy.
Methods: 100 male students were randomly selected from 445 newly enrolled males of Dalian Medical University in 2011 to test their serum testosterone levels, and 100 male students were all healthy subjects. After consulting the consent of the subjects, the subjects were excluded from the group with no history of endocrine and reproductive diseases, no history of mental illness, no history of trauma, and no history of other physical diseases. The age was limited to 17-19 years old. They were right-handed and had no significant difference in intelligence level. The experiment was divided into two tasks. The first task was to stimulate the content of letters, the second task was to judge the spatial position. Both types of stimulus tasks had 150 stimulus sequences (Trial), in which the probability of target stimulus and non-target stimulus appeared randomly (50% each), and there were 15 stimuli in non-target stimulus. It takes about 10 minutes to complete a task, 3 minutes to rest, and about 30 minutes to complete the whole experiment. Analysis and further analysis afterwards.
Result:
(1) There were no significant differences in hitting rate, false alarm rate and interference stimulus response time between high and low testosterone groups, but there were significant differences in stimulus response time and hit rate between high and low testosterone groups. The high testosterone group was significantly higher than the low testosterone group, but there was no significant difference in response time, false alarm rate and interference stimulus response time.
(2) In the waveform diagram, there was no difference in activation between left, middle and right in the high serum testosterone level group under the letter-matching stimulation task, but there was a significant difference in activation between left, middle and right in the low serum testosterone level group, mainly in the frontal, central and parietal regions (F4, C4, P4, PZ). The activation amplitude in the central frontal region was significantly larger than that in the left and right frontal regions, and in the central parietal region was significantly larger than that in the left and right parietal regions.
(3) In the Average Brain Electrical Activity Map (AEEG) evoked by the spatial and alphabetic matching tasks between 280 and 400ms, the difference of serum testosterone level between the two groups was obvious, but the difference of the alphabetic matching task was not obvious. The activation of the high serum testosterone level group was more obvious in the spatial matching task, but in the alphabetic matching task. The activation of low serum testosterone level group was stronger than that of low serum testosterone level group. In addition, the activation region of low serum testosterone level group was inclined to the right hemisphere in the two matching tasks, which was more obvious in the letter matching task, while the activation region of high serum testosterone level group was mainly concentrated in the central region and inclined to both sides. It's not obvious. It's also shown that the most active areas are in the parietal region, while the central and prefrontal regions are weaker.
(4) Average EEG spikes induced by spatial and letter matching tasks between 280 and 400ms were statistically analyzed. In the letter matching task, the main effect of cerebral lobe was significant, the main effect of brain regions and groups was not significant, and the interaction effect of brain regions and groups was not significant. The main effects of regions and groups were not significant, and the interaction effects of brain regions and groups and lobes, brain regions and groups were significant, but other statistical results were not significant.
Conclusion:
1. In alphabet matching task, the low testosterone level group showed compensatory dominant activation in the right hemisphere, while the high testosterone level group, left and right processing at the same time, the difference was not significant.
2. in the spatial matching task, the high testosterone group revealed higher processing status, especially in the central and top regions.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R749.24

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