發(fā)布時間：2018-07-13 21:45

【摘要】：目的研究早期應(yīng)用山莨菪堿在大鼠燒傷后腸源性真菌感染的作用,從而為燒傷患者臨床治療提供新方法的依據(jù)。方法選取健康大鼠72只,隨機分成3組,A組健康鼠真菌液灌胃,B組燒傷鼠真菌液灌胃,C組燒傷鼠真菌液灌胃5分鐘內(nèi)山莨菪堿(3mg/kg)腹腔注射,設(shè)計嚴重燒傷大鼠動物模型,選用標準白色念珠菌菌株制備一定濃度,給燒傷大鼠灌腸,分不同時段(6h,12h,24h,48h)處死,取各組大鼠內(nèi)臟組織,肺部組織進行MPO ELISA半定量測定,小腸粘膜進行HSP-70ELISA半定量測定,并應(yīng)用實時熒光定量PCR技術(shù)對腸道粘膜、肺、肝、腎臟真菌含量進行檢測,同時抽血測定血清丙氨酸轉(zhuǎn)移酶(ALT)、天門冬氨酸轉(zhuǎn)移酶(AST)、肌酐(CRE)尿素氮(BUN),分析不同時相點的組織真菌含量變化,臟器功能指標變化,以探討山莨菪堿對嚴重燒傷大鼠燒傷后腸源性真菌感染的作用。結(jié)果肺組織MPO測定結(jié)果顯示B組(燒傷真菌液灌胃)、C組(燒傷鼠真菌液灌胃5分鐘內(nèi)山莨菪堿腹腔注射)各時相點MPO含量顯著高于A組(健康鼠真菌液灌胃)；B組MPO含量顯著高于C組。小腸粘膜HSP70測定結(jié)果顯示各時相點B組、C組含量明顯高于A組；而且C組含量高于B組。丙氨酸轉(zhuǎn)移酶(ALT)、天門冬氨酸轉(zhuǎn)移酶(AST)B組顯著高于C組高于A組。血清肌酐(CRE)尿素氮(BUN)測定結(jié)果顯示B組、C組在24、48明顯升高,與A組比較有顯著差異,C組與B組比較C組明顯低于B組；各臟器熒光定量PCR檢測真菌顯示均有不同程度侵襲,侵襲程度A組最輕、C組次之,B組最重。結(jié)論嚴重燒傷大鼠腸源性真菌感染后真菌侵襲范圍廣,隨時間延長受感染臟器損傷程度加重；早期使用山莨菪堿可減輕燒傷大鼠的腸源性真菌感染程度,減輕其它臟器的感染程度,保護損傷臟器；實時熒光定量PCR法可作為一種快速檢測方法應(yīng)用于真菌感染的診斷。
[Abstract]:Objective to study the effect of early application of anisodamine on intestinal fungal infection after burn in rats, so as to provide a new method for clinical treatment of burn patients. Methods Seventy-two healthy rats were randomly divided into 3 groups: group A, normal rats were treated with fungous fluid in group B, group C were given intraperitoneal injection of anisodamine (3mg/kg) within 5 minutes, and the animal model of severe burn was designed. Standard Candida albicans strain was used to prepare a certain concentration of Candida albicans. The rats were enema and killed in different time periods (6 h, 12 h, 24 h, 48 h). The visceral tissue and lung tissue of each group were measured by MPO Elisa, and the intestinal mucosa was detected by HSP-70 Elisa. The fungal contents of intestinal mucosa, lung, liver and kidney were detected by real-time fluorescent quantitative PCR. Serum alanine transferase (alt), aspartate transferase (AST), creatinine (CRE) urea nitrogen (bun) were measured. To investigate the effect of anisodamine on intestinal fungal infection after severe burn in rats. Results MPO of lung tissue in group B was significantly higher than that in group A (intraperitoneal injection of anisodamine within 5 minutes). MPO content in group B was significantly higher than that in group C. The results of HSP70 assay showed that the content of HSP70 in group B was significantly higher than that in group A, and that in group C was higher than that in group B. Alanine transferase (alt) and aspartate transferase (AST) were significantly higher in group B than in group C than in group A. The serum creatinine (CRE) urea nitrogen (bun) level in group B was significantly higher than that in group A, which was significantly lower in group C than in group B, and in group C was significantly lower than that in group B. The degree of invasion was the lightest in group A and the lowest in group C, followed by group B. Conclusion severe burn rats have a wide range of fungal invasion after enterogenous fungi infection, and the degree of organ injury is aggravated with time, and early use of anisodamine can reduce the degree of intestinal fungal infection in burn rats. The real-time fluorescence quantitative PCR can be used as a rapid detection method for the diagnosis of fungal infection.
【學位授予單位】：內(nèi)蒙古大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R644;R965

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