發(fā)布時間：2018-06-09 15:04

  本文選題：肺氣腫 + 腫瘤壞死因子α��； 參考：《天津醫(yī)藥》2014年05期

【摘要】：目的建立間歇低氧(IH)合并肺氣腫重疊綜合征(OS)大鼠模型,探討OS大鼠系統(tǒng)及血管內(nèi)皮炎癥狀態(tài),并觀察外周血內(nèi)皮祖細胞(EPC)水平的變化。方法自制熏箱對大鼠進行16周的熏煙暴露造成大鼠肺氣腫,從13周開始,在熏煙暴露同時,通過程控產(chǎn)生預(yù)制的間歇低氧/再氧合(IH/ROX)環(huán)境對大鼠進行IH暴露4周。60只雄性Wistar大鼠隨機分為正常組(A組)、IH組(B組)、肺氣腫組(C組)和OS組(D組),暴露結(jié)束后分別以ELISA法測定血漿及右頸總動脈內(nèi)皮細胞中腫瘤壞死因子(TNF)-α、白介素(IL)-6水平,Real-time PCR法檢測血管內(nèi)皮細胞中RhoA mRNA含量,病理標(biāo)本中測定頸動脈內(nèi)中膜厚度(IMT)占全層厚度比值(C-IMT%),流式細胞儀測定循環(huán)血中EPC數(shù)量。結(jié)果 D組血漿和血管內(nèi)皮細胞中TNF-α、IL-6水平以及血管內(nèi)皮細胞中RhoA mRNA水平、C-IMT%均高于A、B、C組,而EPC數(shù)量均低于其他3組(均P0.05)。結(jié)論 OS大鼠較單純IH或單純肺氣腫大鼠系統(tǒng)及內(nèi)皮損傷更加嚴(yán)重,且內(nèi)皮修復(fù)能力更差,增加了心血管疾病風(fēng)險。
[Abstract]:Objective to establish a rat model of intermittent hypoxic IH (IH) combined with emphysema overlap syndrome (OS) and to investigate the inflammatory status of systemic and vascular endothelial cells in OS rats and to observe the changes of EPCs in peripheral blood endothelial progenitor cells (EPC). Methods the rats were exposed to fumigation for 16 weeks in a self-made fumigation box, which caused emphysema in the rats, and at the same time, the emphysema was induced at the same time as the smoke exposure at the beginning of 13 weeks. By programmed production of prefabricated intermittent hypoxia / reoxygenation (IH / Rox) environment, rats were exposed to IH for 4 weeks. 60 male Wistar rats were randomly divided into two groups: normal group (group A), IH group (group B), emphysema group (group C) and OS group (group D). Tumor necrosis factor TNF- 偽 in plasma and endothelium of right carotid artery was detected by Elisa, and RHPA mRNA in vascular endothelial cells was detected by real-time PCR at IL-6 level. The ratio of IMT to whole layer thickness of carotid artery was measured in pathological specimens and the quantity of EPC in circulating blood was measured by flow cytometry. Results the levels of TNF- 偽 and IL-6 in plasma and vascular endothelial cells and the level of RhoA mRNA in vascular endothelial cells in group D were higher than those in group A, and the number of EPC in group D was lower than that in group A (P 0.05). Conclusion the injury of system and endothelium in OS rats is more serious than that in simple IH or emphysema rats, and the ability of endothelial repair is worse, which increases the risk of cardiovascular disease.
【作者單位】： 天津醫(yī)科大學(xué)總醫(yī)院呼吸科;天津醫(yī)科大學(xué)研究生院;
【基金】：國家自然科學(xué)基金資助項目(項目編號:81270144,30800507,81170071)
【分類號】：R563.3

【相似文獻】

相關(guān)期刊論文 前10條

1 王|蘭;芳香族烴類經(jīng)皮膚的滲透量[J];國外醫(yī)學(xué).衛(wèi)生學(xué)分冊;1982年01期

2 張寅恭;新殺蟲劑Пиримор的衛(wèi)生毒理學(xué)[J];國外醫(yī)學(xué).衛(wèi)生學(xué)分冊;1982年01期

3 蔡海英,張金生,崔小邢,鄭國墚,黃孔威;國產(chǎn)維甲酸衍生物對大鼠食管上皮癌變的抑制作用[J];癌癥;1983年04期

4 高強;大鼠直腸給心得安以避免首過消除效應(yīng)[J];國外醫(yī)學(xué).藥學(xué)分冊;1983年01期

5 Seigo SHUMIYA;Sumi NAGASE;蔡華琬;;大鼠(Rattus Norvegicus)無白蛋白基因(Aualbumia)和蓋帽基因(Hooded)的連鎖[J];實驗動物與比較醫(yī)學(xué);1983年01期

6 陳國志,黃淑英,王廣義,劉志紅;豚鼠和大鼠小腸移行性肌電復(fù)合波(MMC)的觀察[J];基礎(chǔ)醫(yī)學(xué)與臨床;1984年03期

7 橫路謙次郎;不同類型輻射照射復(fù)合催乳素誘發(fā)大鼠乳腺腫瘤的效果[J];輻射防護;1984年03期

8 溫志永;;幼鼠夜間PR 峰的出現(xiàn)對孕酮的依賴作用[J];國外醫(yī)學(xué).婦產(chǎn)科學(xué)分冊;1989年05期

9 趙學(xué)增;鄭富穩(wěn);夏燁;;普萘洛爾對雌性大鼠生殖機能的影響[J];河北醫(yī)科大學(xué)學(xué)報;1989年03期

10 鄭培敏;熊克仁;江家元;;大鼠斜角帶核水平支的傳入和傳出聯(lián)系——WGA—HRP和HRP法研究[J];皖南醫(yī)學(xué)院學(xué)報;1989年04期

相關(guān)會議論文 前10條

1 程井軍;吳其愷;彭銳;孫國杰;;電針對非酒精性脂肪性肝炎大鼠肝組織氧化及抗氧化狀態(tài)的影響[A];中華中醫(yī)藥學(xué)會第十三屆內(nèi)科肝膽病學(xué)術(shù)會議論文匯編[C];2008年

2 楊s，

本文編號：2000134