DGCR8在先天性心臟病患者血液及心肌組織中的表達
[Abstract]:Objective: to investigate the relationship between DGCR8 gene and congenital heart disease (CHD) and its clinical significance. Methods: the expression of DGCR8 mRNA was detected by qRT-PCR in 40 children with CHD and 40 healthy children, 25 patients with ventricular septal defect (VSD) and 16 children with tetralogy of Fallot (TOF). The expression of DGCR8 mRNA and protein was detected by qRT-PCR and Western blot. The correlation between the expression level of DGCR8 and the size of cardiac septal defect in children with septal defect was analyzed. Results: compared with healthy children, the expression of DGCR8 mRNA in the blood of CHD children was decreased (P0. 037), and the expression of DGCR8 mRNA and protein in myocardial tissue of children with TOF was lower than that in children with VSD (P0.05). There was no significant correlation between DGCR8 mRNA expression and cardiac septal defect (rS- 0.022P0.917). Conclusion the deletion of the DGCR8 gene is related to the occurrence of CHD and affects the normal development of the heart.
【作者單位】: 鄭州大學(xué)第三附屬醫(yī)院婦產(chǎn)科;田納西大學(xué)健康科學(xué)中心病理醫(yī)學(xué)實驗室;河南省人民醫(yī)院兒童心臟中心;河南省婦科腫瘤納米醫(yī)學(xué)國際聯(lián)合實驗室;
【基金】:國家自然科學(xué)基金面上項目81572574 河南省高校科技創(chuàng)新團隊項目16IRTSTHN018 鄭州大學(xué)創(chuàng)新項目DYCX053
【分類號】:R725.4
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