本文選題：牦牛 + 肺臟�。� 參考：《甘肅農業(yè)大學》2017年碩士論文

【摘要】：實驗目的:本研究旨在探討牦牛肺臟對高原低氧環(huán)境的應答機制。為了研究牦牛肺臟結構及其對低氧適應性的關系,本研究選擇對肺臟結締組織和平滑肌增生具有特定作用的CTGF(Connective Tissue Growth Factor)和FGF-2(Fibroblast Growth Factors-2)兩種因子。本研究初步探討CTGF與FGF-2對牦牛肺臟低氧適應性結構形成中的作用。實驗方法:采用EVG特殊染色方法觀察初生、3-5月齡和成年牦牛肺臟內纖維成分的分布情況;免疫組化技術對CTGF和FGF-2在三個年齡段牦牛肺臟內準確的分布時間、位置和表達量進行研究。結果:⑴初生、3-5月齡和成年牦牛肺臟中,膠原纖維主要分布在支氣管各級分支和肺動脈周圍。彈性纖維分布于肺動脈外膜與中膜的交界處。三個年齡段相比,3-5月齡組支氣管各級和肺動脈的肌層增厚明顯。⑵在牦牛肺臟支氣管和細支氣管中,CTGF主要表達在上皮細胞,其中3-5月齡組均表達最強(P0.05),初生次之,成年最弱,但初生和成年無顯著差異性(P0.05)。CTGF少量分布在肺血管內皮。在肺小動脈(直徑為50um)中,三個年齡組CTGF表達呈緩慢降低趨勢(P0.05)。CTGF在3-5月齡組肺動脈(100um~200um)表達最強(P0.05),成年次之,初生最少(P0.05)。3-5月齡組肺動脈(200um)CTGF表達強度最強,成年次之,初生最少(P0.05)。⑶在支氣管各級分支中,FGF-2主要分布在上皮細胞和平滑肌細胞。3-5月齡組支氣管表達程度最強(P0.05),初生次之(P0.05),成年最弱(P0.05)。3-5月齡組細支氣管表達最強(P0.05),初生次之且與成年組無顯著性差異(P0.05)。在各級肺動脈中,FGF-2在3-5月齡組肺小動脈(50um)的表達量最高,初生次之,成年組極低(P0.05)。初生、3-5月齡以及成年三組肺動脈(100um~200um)的FGF-2表達量緩慢增加(P0.05)。3-5月齡組肺動脈(200um)表達量最高(P0.05),初生最少(P0.05)。結論:⑴初生、3-5月齡和成年牦牛肺臟的支氣管、細支氣管及其分支,小動脈(50um)、肺動脈(100um~200um)以及大動脈(200um)管壁均有大量膠原纖維和彈性纖維分布,且血管壁內平滑肌較厚,造成肺動脈及其分支的血管壁均較厚,血管強度高。⑵CTGF主要分布于各年齡段牦牛肺內支氣管及其分支的上皮細胞、肺動脈內皮細胞,且在3-5月齡分布最多,說明CTGF可能參與了牦牛氣道和肺動脈的形成。⑶FGF-2主要分布于各年齡段牦牛肺內支氣管及其分支的上皮細胞、肺動脈內皮細胞;在肺內支氣管外膜平滑肌和肺動脈平滑肌中也有大量分布;在3-5月齡的分布量最多。說明該因子參與了牦牛氣道和肺動脈的結構的形成。
[Abstract]:Objective: to investigate the mechanism of yak lung response to high altitude hypoxia. In order to study the relationship between lung structure and hypoxia adaptation of yak, two factors, CTGF (Connective tissue growth Factor) and FGF-2 (fibroblast growth Factors-2), which play a specific role in pulmonary connective tissue and smooth muscle hyperplasia, were selected in this study. The purpose of this study was to investigate the role of CTGF and FGF-2 in the formation of hypoxic adaptive structure in yak lung. Methods: using EVG special staining method to observe the distribution of fiber components in the lungs of the newborn and adult yaks, and to determine the exact distribution time of CTGF and FGF-2 in the lungs of the three age groups. Position and expression were studied. Results the collagen fibers were mainly distributed in the bronchi branches and around the pulmonary artery in the lung of the yak at the age of 3-5 months and adult yak. Elastic fibers are distributed at the junction of the adventitia and the medial membrane of the pulmonary artery. The myometrium thickening of bronchi and pulmonary artery in the three age groups was significantly higher than that in the 3-5 month group. CTGF was mainly expressed in the epithelial cells in the bronchi and bronchioles of the yak. The expression of CTGF was the highest in the 3-5 month group (P0.05), followed by the primary age group, and the weakest in adult group. However, there was no significant difference between primary and adult (P0.05). CTGF was distributed in pulmonary vascular endothelium. In pulmonary arterioles (diameter: 50um), the expression of CTGF in three age groups showed a slow decreasing trend (P0.05). The expression of CTGF was the strongest in the pulmonary artery (100um~200um) of 3-5 months old group (P0.05), followed by the adult group (P0.05), the lowest (P0.05) .3-5 month old group (200um) expression intensity was the highest, and the adult group was the second. FGF-2 mainly distributed in epithelial cells and smooth muscle cells. 3-5 months old group (P0.05), newborn group (P0.05), adult weakest (P0.05) .3-5 months old group bronchiole expression (P0.05), primary stage of birth. There was no significant difference between the two groups (P0.05). The expression of FGF-2 was the highest in the pulmonary arterioles (50um) of 3-5 months old group, followed by the newborn group, and was very low in the adult group (P0.05). The expression of FGF-2 in pulmonary artery (100um~200um) of 3 to 5 months old and adult group increased slowly (P0.05). The expression of 200um was the highest in the group of 3-5 months of age (P0.05), and the lowest in newborn group (P0.05). Conclusion the bronchioles, bronchioles and their branches, arterioles (50um), pulmonary arteries (100um~200um) and great arteries (200um) in the lung of the yak between 3-5 months and 3 to 5 months of age have a large number of collagen fibers and elastic fibers, and the smooth muscle of the vascular wall is thicker. The wall of pulmonary artery and its branches were thicker. The high intensity of CTGF was mainly distributed in the epithelial cells of the bronchi and its branches of the lung of yak in all ages, and the endothelial cells of the pulmonary artery were most distributed at the age of 3 to 5 months. These results suggest that CTGF may be involved in the formation of airway and pulmonary arteries. 3FGF-2 mainly distributes in epithelial cells and pulmonary artery endothelial cells in the lung bronchi and its branches of yak in all ages. There were also a large number of distributions in pulmonary adventitia smooth muscle and pulmonary artery smooth muscle, and the distribution was the most in 3-5 months old. It is suggested that this factor is involved in the formation of airway and pulmonary arteries in yak.
【學位授予單位】：甘肅農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S823.85
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本文編號：2094547