本文選題：隱孢子蟲 + S�。� 參考：《中國(guó)獸醫(yī)科學(xué)》2017年03期

【摘要】：為了解貴陽(yáng)動(dòng)物園圈養(yǎng)野生動(dòng)物隱孢子蟲的感染流行情況,以駱駝、黑熊、斑馬、袋鼠、獼猴、長(zhǎng)臂猿、大象等野生動(dòng)物的新鮮糞便為樣品(n=84),采用飽和蔗糖溶液漂浮法收集隱孢子蟲的卵囊并提取D N A,以18S r R NA為目標(biāo)基因靶點(diǎn)設(shè)計(jì)特異性引物,并采用巢式PCR擴(kuò)增目的基因,后經(jīng)10 g/L瓊脂糖凝膠電泳,對(duì)檢測(cè)結(jié)果為陽(yáng)性的PCR產(chǎn)物進(jìn)行序列分析并建立系統(tǒng)發(fā)育樹。結(jié)果表明,共有13份隱孢子蟲陽(yáng)性樣品,其中駱駝7份,感染率為70.00%,感染蟲種為安氏隱孢子蟲;斑馬1份,感染率為10.00%,感染蟲種為安氏隱孢子蟲;長(zhǎng)臂猿5份,感染率為23.81%,感染蟲種為微小隱孢子蟲、人隱孢子蟲及小鼠隱孢子蟲。本次調(diào)查發(fā)現(xiàn)該園野生動(dòng)物隱孢子蟲具有較高的總體感染率(15.50%)和鑒定出4種人獸共患隱孢子蟲蟲種,具有較重要的公共衛(wèi)生意義。本研究首次報(bào)道了我國(guó)斑馬感染隱孢子蟲。
[Abstract]:In order to understand the prevalence of cryptosporidium in captive wild animals in Guiyang Zoo, camels, black bears, zebras, kangaroos, macaques, gibbons, Fresh feces from wild animals such as elephants were used as samples. The oocysts of Cryptosporidium were collected by saturated sucrose solution floating method. The specific primers were designed with 18s r R NA as the target gene target, and the target genes were amplified by nested PCR. After 10 g / L agarose gel electrophoresis, the positive PCR products were sequenced and phylogenetic tree was established. The results showed that there were 13 positive samples of Cryptosporidium, of which 7 were camels, the infection rate was 70.00.The infection rate was Cryptosporidium andersoni, one zebra, the infection rate was 10.00, the infection rate was Cryptosporidium Angiosporium, and the gibbon was 5. The infection rate was 23.81%. The infected species were Cryptosporidium minimus, Cryptosporidium human and Cryptosporidium mouse. This investigation found that the wild animal Cryptosporidium has a higher total infection rate of 15.50%) and identified four species of zoonotic Cryptosporidium species, which has important public health significance. This study reported for the first time that zebras were infected with Cryptosporidium in China.
【作者單位】： 四川農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)院動(dòng)物疫病與人類健康四川省重點(diǎn)實(shí)驗(yàn)室;重慶市石柱縣畜牧產(chǎn)業(yè)發(fā)展中心西沱鎮(zhèn)畜牧獸醫(yī)站;成都大熊貓繁育研究基地;四川省名山區(qū)農(nóng)業(yè)局;
【基金】：成都大熊貓繁育研究基金會(huì)項(xiàng)目(CPF2014-14) 國(guó)家自然科學(xué)基金項(xiàng)目(31272620)
【分類號(hào)】：S852.723

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