本文選題：卵巢 + 黃體囊腫�。� 參考：《核農(nóng)學(xué)報(bào)》2017年01期

【摘要】：為了研究奶牛黃體囊腫的發(fā)病機(jī)理及發(fā)病時(shí)的生物學(xué)標(biāo)記物,利用蛋白質(zhì)組學(xué)經(jīng)典的雙向電泳(2-DE)技術(shù),檢測(cè)和分析奶牛黃體囊腫組織和正常黃體組織間的差異蛋白,通過考馬斯亮藍(lán)染色后用PDQuest8.0軟件檢測(cè)并分析差異蛋白點(diǎn)。經(jīng)MALDI TOF/TOF-MS/MS串聯(lián)質(zhì)譜鑒定,共發(fā)現(xiàn)6種差異表達(dá)的蛋白,分別為:α-1-酸性糖蛋白(AGP),膠質(zhì)纖維酸性蛋白(GFAP),核磷蛋白(NPM),泛素羧基末端水解酶(UCH),牛血清白蛋白晶體結(jié)構(gòu)A鏈(Chain A),絲氨酸蛋白酶抑制蛋白A3-5(serpin A3-5)。其中,AGP、GFAP、serpin A3-5、NPM和UCH在囊腫組織中高表達(dá),Chain A只在正常組織中表達(dá)。通過分析這些蛋白在組織間的表達(dá)差異,發(fā)現(xiàn)其可能與黃體囊腫的發(fā)生和囊腫發(fā)生后激素分泌異常有關(guān)。黃體囊腫差異蛋白的發(fā)現(xiàn)為奶牛黃體囊腫臨床診斷及發(fā)生機(jī)理的研究提供了科學(xué)依據(jù)。
[Abstract]:In order to study the pathogenesis and biological markers of luteal cyst in dairy cattle, differential proteins were detected and analyzed between the tissues of luteal cyst and normal corpus luteum by using the proteomics technique of two-dimensional electrophoresis (2-DEE).The differential protein spots were detected and analyzed by PDQuest8.0 software after Coomassie brilliant blue staining.Six differentially expressed proteins were identified by MALDI TOF/TOF-MS/MS tandem mass spectrometry.They were 偽 -1-acidic glycoprotein (AGP), glial fibrillary acidic protein (GFAP), nucleophosphorus protein (NPP), ubiquitin carboxyl terminal hydrolase (UCHN), crystal structure of bovine serum albumin (BSA) A chain chain An, serine protease inhibitor protein (A3-5(serpin A3-5).UCH and UCH were highly expressed in cysts only in normal tissues.By analyzing the differences in the expression of these proteins among tissues, it was found that these proteins might be related to the occurrence of luteal cyst and the abnormal hormone secretion after cysts.The discovery of differential protein in luteal cyst provides scientific basis for clinical diagnosis and mechanism of luteal cyst in dairy cattle.
【作者單位】： 寧夏大學(xué)農(nóng)學(xué)院;寧夏吳忠市利通區(qū)農(nóng)牧局扁擔(dān)溝畜牧獸醫(yī)站;
【基金】：國(guó)家自然科學(xué)基金(31160523)
【分類號(hào)】：S858.23

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