本文選題：鴨疫里默氏桿菌　切入點：對數(shù)生長期　出處：《黑龍江畜牧獸醫(yī)》2017年05期 　論文類型：期刊論文

【摘要】：為了研究常用抗生素對臨床分離到的鴨疫里默氏桿菌(riemerella anatipestifer,RA)的抑菌效果,試驗首先繪制RA的生長曲線,確定其對數(shù)生長期,以對數(shù)生長期的細菌作為受試菌,測定6種常用抗生素對4株RA菌株在不同稀釋度時的最小抑菌濃度(MIC),確定最佳接菌量,進而確定測定MIC的最佳時間。結(jié)果表明:新鮮培養(yǎng)的RA菌液的OD600值為0.8~1.2時,細菌達到對數(shù)生長期,菌落形成單位達到1×10~(11)~1×10~(12)cfu/m L。對數(shù)生長期細菌稀釋至1×10~(-5)為最佳接菌量,將上述菌液接種到96孔板后,在37℃培養(yǎng)22~24 h為檢測MIC的最佳時間。
[Abstract]:In order to study the bacteriostatic effect of common antibiotics on riemerella anatipestifera isolated from clinic, the growth curve of RA was drawn first, and the logarithmic growth period was determined, and the bacteria in logarithmic growth period were used as the tested bacteria. The minimal inhibitory concentration (MIC) of 6 common antibiotics against 4 RA strains with different dilution was determined, the optimum inoculation amount was determined, and the optimal time for MIC determination was determined. The results showed that the OD600 value of fresh cultured RA was 0.81.2. When bacteria reached logarithmic growth stage, colony forming unit reached 1 脳 10 ~ (10) ~ (11) ~ 1 脳 10 ~ (-1) C _ (F ~ (-1) 路L ~ (-1)) in logarithmic growth phase, the optimal inoculation amount was 1 脳 10 ~ (10) ~ (-5). After inoculating the above bacteria to 96-well plate, the optimal time for MIC detection was 22 ~ 24 h at 37 鈩，

本文編號：1607072