【摘要】：目的通過檢測(cè)不同慢性乙型肝炎病毒(HBV)感染階段的患者外周血單核細(xì)胞(PBMCs)表面程序性死亡受體-1(PD-1)及其配體(PD-L1)的表達(dá),探討其與血清HBV-DNA載量、ALT之間的相關(guān)性。方法收集不同臨床類型慢性HBV感染者80例,其中HBV攜帶者20例(A組),1×ULN≤ALT2×ULN 20例(B組),2×ULN≤ALT5×ULN 20例(C組),ALT≥5×ULN 20例(D組),健康獻(xiàn)血者20例(E組)。采用流式細(xì)胞術(shù)檢測(cè)外周血單核細(xì)胞(PBMCs)表面PD-1及其配體PD-L1表達(dá),以實(shí)時(shí)熒光定量PCR檢測(cè)血清HBVDNA,同時(shí)檢測(cè)血清谷丙轉(zhuǎn)氨酶(ALT)。結(jié)果 A、B、C、D組PBMCs表面PD-1、PD-L1表達(dá)顯著高于E組(P0.05);C、D組與A、B組間比較,PBMCs表面PD-1、PD-L1表達(dá)顯著升高(P0.05),C組與D組比較PBMCs表面PD-1、PD-L1表達(dá)水平差異無統(tǒng)計(jì)學(xué)意義(P0.05);A組與B組比較,PBMCs表面PD-1、PD-L1表達(dá)水平差異無統(tǒng)計(jì)學(xué)意義(P0.05)。不同慢性HBV感染階段患者PBMCs表面PD-1、PD-L1表達(dá)水平與血清HBV-DNA載量間無相關(guān)性(r=0.271,P0.05),但與ALT間有相關(guān)性(r=0.679,P0.05)。結(jié)論慢性HBV不同感染階段患者PBMCs表面PD-1、PD-L1表達(dá)與機(jī)體免疫狀態(tài)有關(guān),肝臟炎癥損傷是影響PD-1、PD-L1表達(dá)的重要因素,HBV-DNA載量不是主要影響原因。
[Abstract]:Objective to investigate the expression of programmed death receptor 1 (PD-1) and its ligand (PD-L1) on the surface of peripheral blood monocytes of patients with chronic hepatitis B virus (HBV) infection at different stages of (PBMCs) infection, and to investigate their association with serum HBV-DNA load. The correlation between ALT. Methods 80 patients with different clinical types of chronic HBV infection were collected, including 20 HBV carriers (group A), 1 脳 ULN 鈮，

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