【摘要】：目前,病原菌仍是人類健康最大的威脅之一,比如急性呼吸道感染、腹瀉、肺結(jié)核等疾病都是由于病原菌的感染所致。而且,新的細(xì)菌感染性疾病的不斷出現(xiàn),以及大量致病菌的反復(fù)出現(xiàn)和耐藥性的增加,對(duì)公眾健康造成可怕的威脅。因此,對(duì)潛在病原菌的發(fā)現(xiàn)以及病原菌致病機(jī)理的研究一直是研究的熱點(diǎn)之一。 人紅白血病細(xì)胞系K562細(xì)胞被廣泛用于實(shí)驗(yàn)室的研究。當(dāng)我們對(duì)K562細(xì)胞進(jìn)行核型分析過(guò)程中,意外地發(fā)現(xiàn)K562細(xì)胞染色標(biāo)本中有桿狀細(xì)菌樣的結(jié)構(gòu)。為了排除該實(shí)驗(yàn)中相關(guān)實(shí)驗(yàn)試劑的環(huán)境細(xì)菌污染,我們嚴(yán)格控制了各種實(shí)驗(yàn)試劑和實(shí)驗(yàn)操作。然而,多次重復(fù)實(shí)驗(yàn)仍得到以上結(jié)果。因此,我們推論該細(xì)胞中可能存在一種細(xì)胞內(nèi)生菌。將K562細(xì)胞、RPMI 1640完全培養(yǎng)基以及各種實(shí)驗(yàn)用試劑分別涂至基礎(chǔ)血平板(5%綿羊血)上,置37℃孵箱中培養(yǎng)48～72小時(shí),結(jié)果發(fā)現(xiàn)涂有K562細(xì)胞的平板上有菌落生成,而其它平板均無(wú)任何菌落形成。經(jīng)多次實(shí)驗(yàn)證明,平板上形成的細(xì)菌集落確為K562細(xì)胞來(lái)源。只得注意的是,K562細(xì)胞能保持良好的生長(zhǎng)狀態(tài)和增殖能力,而且細(xì)胞培養(yǎng)基中無(wú)明顯的細(xì)菌污染跡象。以上初步的實(shí)驗(yàn)結(jié)果提示,寄生在K562細(xì)胞內(nèi)的細(xì)菌可能是一種新的細(xì)胞內(nèi)生菌,因?yàn)槟壳耙衙鞔_的能寄生在人類細(xì)胞內(nèi)的胞內(nèi)寄生菌在細(xì)胞內(nèi)會(huì)大量繁殖,因此感染的細(xì)胞不能健康生長(zhǎng)。由于該菌可能是一種新的胞內(nèi)寄生菌,就有必要明確該菌在細(xì)菌分類中的地位,以及該菌的基本的生物學(xué)特性。 第一部分:HLK1~T的分離和鑒定 目的:對(duì)細(xì)菌的分類鑒定是了解其生物學(xué)功能和特性的基礎(chǔ)。為明確HLK1~T的分類地位及其基本生物學(xué)特性,該研究從人紅白血病細(xì)胞系562細(xì)胞中分離獲得
[Abstract]:At present, the pathogenic bacteria are still one of the greatest threats to human health, such as acute respiratory tract infection, diarrhea, pulmonary tuberculosis and other diseases due to the infection of the pathogenic bacteria. Moreover, the emergence of new bacterial infectious diseases, as well as the repeated appearance and resistance of a large number of pathogenic bacteria, poses a terrible threat to public health. Therefore, the research on the discovery of the potential pathogenic bacteria and the pathogenesis of the pathogenic bacteria has been one of the hot spots of the study. Human erythroleukemia cell line K562 cells are widely used It was used in the research of the laboratory. In the process of the karyotype analysis of K562 cells, it was found that the K562 cells were stained with staining. The structure of the rod-shaped bacteria-like structure. In order to eliminate the environmental bacterial contamination of the relevant experimental reagents in the experiment, we have strictly controlled the various facts The test reagent and the experimental operation. However, the test is repeated many times. The above results are still available. Therefore, we can infer that the cells may be stored in the cell In a cell endophytic bacterium, K562 cells, RPMI 1640 full culture medium, and various experimental reagents are respectively coated on a basic blood plate (5% sheep blood), and incubated for 48-72 hours in a 37-degree-C cold box, and the results show that the plate coated with K562 cells has colony formation, while other plates None of the colonies were formed. The colony of bacteria formed on the plate was proved to be true by multiple experiments. The K562 cell is a source of cell. It is important to note that K562 cells can maintain good growth and proliferation, and the cell culture medium is not clear. The above preliminary experimental results suggest that the bacteria that are parasitic in the K562 cells may be a new endogenetic strain, as the intracellular parasitic bacteria that can be parasitically in the human cell can be propagated in a large amount in the cell, thus infecting The cell cannot grow well. As the bacteria may be a new intracellular parasitic, it is necessary to identify the status of the bacterium in the bacterial classification, and the bacteria. Basic biological characteristics. Part 1: Separation and identification of HLK1-T Objective: The classification and identification of bacteria is the basis of understanding the biological functions and characteristics of the bacteria. In order to clarify the classification status of HLK1-T and its basic life
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類號(hào)】：R378

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