本文選題：RVaG株 + PHKC　； 參考：《吉林大學》2007年碩士論文

【摘要】： 狂犬病病毒屬彈狀病毒科的狂犬病毒屬,它屬于單鏈RNA病毒。是引起人畜共患性傳染病-狂犬病的病原體。自70年代以來,有關狂犬病街毒株及固定毒株在動物體內(nèi)及培養(yǎng)細胞內(nèi)的病毒抗原的熒光抗體染色及其病毒超微結(jié)構(gòu)的研究陸續(xù)有報道[1-3]。但廣泛應用于生產(chǎn)狂犬細胞培養(yǎng)疫苗的固定毒aG株在原代地鼠腎細胞內(nèi)病毒復制部位、病毒增殖動態(tài)、病毒的超微結(jié)構(gòu)、病毒形態(tài)發(fā)生以及光鏡下病毒抗原定位與電鏡下病毒復制部位的關系等報道很少。掌握病毒感染細胞后的生物學過程對狂犬病的預防、診斷、治療都有重要意義。另外這方面的基礎理論資料對改進生產(chǎn)工藝,提高狂犬疫苗產(chǎn)量和質(zhì)量也有指導意義,同時也為生產(chǎn)和檢定部門提供敏感性高、特異性強,并且簡單快速的狂犬病毒抗原檢測方法。為此,本論文利用現(xiàn)代免疫酶組織化學及電鏡技術進行了系統(tǒng)試驗。本實驗著重對免疫組織化學技術中常用的免疫熒光法和免疫酶組織化學法(ABC法)進行對比試驗。并對光鏡和電鏡觀察結(jié)果進行了比較。
[Abstract]:Rabies virus of the family Granuloviridae, belonging to a single-stranded RNA virus. Is the cause of zoonotic sexually transmitted diseases-rabies pathogens. Since the 1970s, the studies on fluorescent antibody staining and ultrastructure of rabies street virus strains and stationary strains in animal and cultured cells have been reported one after another. But it is widely used in producing rabies cell culture vaccine. The viral replication site, virus proliferation dynamics and virus ultrastructure of the virus in primary hamster kidney cells are the fixed virus AG strain, which is widely used in the production of rabies cell culture vaccine. There are few reports on the relationship between viral morphogenesis and the localization of virus antigen under light microscope and the replication site of virus under electron microscope. It is important to master the biological process of virus infection for the prevention, diagnosis and treatment of rabies. In addition, the basic theoretical data in this respect also have guiding significance for improving the production process and improving the production and quality of rabies vaccine, and also provide high sensitivity and specificity for the production and verification departments. And simple and rapid detection method of rabies virus antigen. Therefore, a systematic experiment was carried out by using modern immunoenzyme histochemistry and electron microscopy. In this experiment, the immunofluorescence method and the immuno-enzyme histochemical method (ABC method), which are commonly used in immunohistochemistry, were studied. The results of light microscope and electron microscope were compared.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R446.6;R392

【參考文獻】

相關期刊論文 前3條

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2 鄒嘯環(huán)，，楊盛華，侯世寬，張仁誠，馬愛民;感染鼠腦神經(jīng)細胞的狂犬病病毒形態(tài)發(fā)生學觀察[J];中國獸醫(yī)科技;1995年10期

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本文編號：2110993