本文選題：口腔潰瘍　切入點(diǎn)：超氧化物岐化酶　出處：《中國(guó)組織工程研究》2014年49期 　論文類(lèi)型：期刊論文

【摘要】：背景:復(fù)發(fā)性阿弗他潰瘍的具體發(fā)病機(jī)制目前醫(yī)學(xué)界尚無(wú)定論,其中體內(nèi)氧自由基的增多是引起復(fù)發(fā)性口腔潰瘍反復(fù)發(fā)作的原因之一。目的:觀察超氧化物歧化酶對(duì)復(fù)發(fā)性阿弗他潰瘍治療效果的影響。方法:在24只復(fù)發(fā)性阿弗他潰瘍家兔模型建立后,按隨機(jī)數(shù)字表法分為3組。模型對(duì)照組作為對(duì)比不做處理;陰性對(duì)照組家兔每天局部碘甘油擦拭;超氧化物歧化酶組家兔每天進(jìn)行超氧化物歧化酶生物膠囊溶液灌胃,潰瘍局部用碘甘油進(jìn)行擦拭。1周后切取口腔潰瘍病變組織進(jìn)行對(duì)比研究。各組家兔造模后,每隔3 d上午同一時(shí)間兔耳靜脈采血,流式細(xì)胞儀分析T細(xì)胞亞群CD3+、CD4+、CD8+以及CD3+/CD8+變化。結(jié)果與結(jié)論:當(dāng)潰瘍病變形成后,模型對(duì)照組和陰性對(duì)照組家兔外周血中細(xì)胞CD3+、CD4+、CD4+/CD8+的比值經(jīng)過(guò)統(tǒng)計(jì)均明顯低于正常,而CD8+細(xì)胞數(shù)遠(yuǎn)遠(yuǎn)大于正常數(shù)值。超氧化物歧化酶組灌服超氧化物歧化酶生物膠囊后,CD3+、CD4+細(xì)胞數(shù)開(kāi)始逐漸升高,而CD8+細(xì)胞數(shù)則相反呈下降趨勢(shì)。提示應(yīng)用氧自由基的天然清除劑超氧化物歧化酶就能有效清除潰瘍面中過(guò)量的氧自由基,從而達(dá)到臨床上治療復(fù)發(fā)性阿弗他潰瘍的目的。
[Abstract]:Background: the specific pathogenesis of recurrent aphthous ulcer is still unknown in the medical field. The increase of oxygen free radicals in the body is one of the causes of recurrent recurrent oral ulcer. Objective: to observe the effect of superoxide dismutase on the treatment of recurrent aphthous ulcer. After the establishment of the rabbit model of fatta ulcer, The model control group was not treated as a control group; the negative control group was given daily iodine glycerol swab; and the superoxide dismutase group was given intragastric administration of superoxide dismutase biological capsule solution every day. The lesion tissues of oral ulcer were removed with iodine glycerine for 1 week and the blood was collected from the ear vein at the same time every 3 days in the morning after each rabbit model was made. Flow cytometry was used to analyze the changes of CD4 / CD8 and CD3 / CD8 in T cell subsets. Results and conclusion: the ratio of CD4 / CD8 in peripheral blood of model control group and negative control group was significantly lower than that of normal control group. However, the number of CD8 cells was much larger than the normal value, and the number of CD3 + CD4 cells in the superoxide dismutase group began to increase gradually after the superoxide dismutase group was administered with biological capsules of superoxide dismutase. The number of CD8 cells decreased on the contrary, suggesting that superoxide dismutase (SOD), a natural scavenger of oxygen free radicals, could effectively scavenge excess oxygen free radicals in the ulcer surface, thus achieving the purpose of clinical treatment of recurrent aphthous ulcer.
【作者單位】： 佳木斯大學(xué)附屬口腔醫(yī)院;佳木斯大學(xué)附屬第一醫(yī)院;
【基金】：黑龍江省衛(wèi)生廳項(xiàng)目(2013215) 2014年度黑龍江省教育廳科學(xué)技術(shù)研究項(xiàng)目計(jì)劃(12541784)~~
【分類(lèi)號(hào)】：R-332;R781.5
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本文編號(hào)：1560158