本文關鍵詞：內臟初級傳入神經和化學表型的多重性　出處：《新疆醫(yī)科大學》2007年碩士論文　論文類型：學位論文

  更多相關文章： 腸神經系統(tǒng) 內臟傷害性刺激感受器 細胞內鈣阻斷蛋白 植物凝集素B4

【摘要】： 目的:探討腸神經系統(tǒng)的內臟初級傳入神經元形態(tài)和神經元的化學表型,以此確定內臟傷害性刺激感受器的神經化超敏作用,為尋找傷害性刺激感受器的化學標記物和探討內臟感覺超敏的神經機制。方法:利用免疫熒光細胞化學技術對BALBc小鼠的腸神經系統(tǒng)小腸和結腸遠端的粘膜下神經叢(SMP)和肌間神經叢(MP)的內臟初級傳入神經元進行人類神經蛋白(HU蛋白),細胞內鈣阻斷蛋白(Calretinin)和植物凝集素B4(IB4)的化學標記。結果:人類神經蛋白能標記所有的內臟初級傳入神經元,Hu蛋白被認為是神經元的常規(guī)陽性標記物。顯示細胞體形態(tài)和胞體的全漿性表達。Calretinin免疫陽性反應作為內臟初級傳入神經元的顯著標記物。Calretinin能很好的顯示細胞的形態(tài),Calretinin標記的顯著特點在于整個細胞以及樹突均勻地分布陽性反應地標記物。Calretinin標記的陽性神經元顯示細胞體和樹突,Hu蛋白標記的神經元表面光滑,而Calretinin免疫陽性神經元表面有突起,免疫反應陽性標記部位從根本把兩者區(qū)分開。而植物凝集素B4免疫反應陽性神經元僅標記細胞膜和胞內顆粒狀物質。IB4免疫陽性部位發(fā)生在細胞膜的表面和細胞內一側的高爾基復合體,形成薄層的細胞膜表面陽性和胞漿內一側的顆粒狀陽性物質。IB4的細胞膜表達從根本上區(qū)別于Calretinin免疫反應陽性的全漿性和胞體表面的多突起表達。三種不同的陽性標記物,免疫反應部位既有雷同又有區(qū)別,使得免疫反應陽性神經元的定性有了確切的保證。通過三種不同的陽性標記物來發(fā)現(xiàn)和確認內臟傷害性刺激感受器的化學表型。結論:植物凝集素B4能與細胞膜上的碳糖類化合物特異性的結合,作為選擇性細胞靶標來證實傷害性刺激信息由內臟初級傳入神經元的傳導。在小鼠的內臟神經系統(tǒng),IB4免疫反應的陽性神經元,能夠被確定為內臟傷害性刺激感受器的初級傳入。IB4標記的陽性神經元對內臟傷害性刺激的感受觸發(fā)了傷害性刺激過程的啟動。內臟的高敏性是腸易激綜合癥(IBS)的主要發(fā)病機制,腸道感覺的高敏性和初級傳入神經沖動地異常在IBS發(fā)病中起重要作用,胃腸動力的變化被認為是IBS最基本的病理生理學異常。在急性疼痛過程中,闡明了痛覺神經元的高敏化機制。
[Abstract]:Objective: To investigate the chemical phenotype of the enteric nervous system visceral afferent neurons and neuron morphology, in order to determine the visceral nociceptive stimulation receptor nerve ultra sensitive function, neural system for chemical markers for nociception and discussion of visceral stimulation hypersensitivity. Methods: BALBc mice intestinal nervous system the small intestine and the distal colon by fluorescent immunocytochemistry in the submucosal plexus (SMP) and myenteric plexus (MP) of primary afferent neurons of human visceral nerve protein (HU protein), intracellular calcium blocker protein (Calretinin) and lectin B4 (IB4) chemical markers. Results: human neuroprotein can mark all the visceral afferent neurons, Hu protein is considered normal positive markers of neurons. Show the morphology and cell body expression of.Calretinin immunoreactivity in the cytoplasm Visceral primary afferent neurons were markers of.Calretinin can show the cell shape is very good, the remarkable characteristics of Calretinin labeled positive neurons and dendritic cells in the uniform distribution of positive markers of.Calretinin markers showed cell bodies and dendrites of neurons, surface Hu protein and Calretinin immunoreactive neurons in smooth surface processes. Immunoreactive markers to distinguish the two parts from the root. The Golgi complex side lectin B4 immunoreactive positive neurons only plant cell membrane and intracellular markers of.IB4 immune positive granular material occurs in the cell membrane surface and inside the cell, the cell membrane formed on the side surface of the cell membrane and cytoplasm positive in the granular layer the expression of.IB4 positive material fundamentally different from the Calretinin immunoreactive pulp and cell surface The expression of surface protrusions. Three different positive markers, immune response at the site of both identical and difference, the qualitative immunoreactive neurons have a guarantee. By three different positive markers to find and confirm the visceral nociceptive stimulation receptor phenotype. Conclusion: chemical plant lectin B4 carbon specific carbohydrate compounds combined with the cell membrane, as a selective target cell to confirm the nociceptive information from the visceral afferent neuron conduction. In the visceral nervous system in mice, IB4 immunoreactive positive neurons, can be identified as visceral noxious stimulation receptor positive neurons in primary afferent.IB4 mark of visceral nociceptive triggered nociceptive process started. Visceral hypersensitivity is irritable bowel syndrome (IBS) is the main pathogenesis of gut feeling The abnormality of high sensitivity and primary afferent nerve impulse plays an important role in the pathogenesis of IBS. The change of gastrointestinal motility is regarded as the most basic pathophysiological abnormality of IBS. In the process of acute pain, the hyperalgesia mechanism of pain neurons is elucidated.

【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R322

【參考文獻】

相關期刊論文 前6條

1 林中,胡宏鎮(zhèn),Jackie D Wood;Hu蛋白作為豚鼠小腸肌間叢神經元標志物的研究[J];基礎醫(yī)學與臨床;2002年03期

2 張育文,王麗娜,李熳,張靜,李玲俐,關新民;炎性痛大鼠背根節(jié)及脊髓后角NMDAR1與BSI-B4免疫熒光雙標記觀察及針刺調節(jié)[J];解剖學報;2005年01期

3 曹東元,牛漢璋,唐向東,李強;電刺激大鼠腓腸神經引起A_δ、C類傳入神經纖維的背根反射[J];生理學報;2003年01期

4 沈駿,諸琦,袁耀宗,張仲偉,陳克敏;腸易激綜合征患者腦部痛覺功能區(qū)域的變化[J];胃腸病學和肝病學雜志;2005年02期

5 曹芝君;莫劍忠;;中樞神經系統(tǒng)異常在腸易激綜合征內臟高敏感性形成中的作用[J];胃腸病學;2006年03期

6 王麗娜,楊建平,張育文,李熳;電針對炎性痛大鼠背根節(jié)BSI-B4表達的調節(jié)觀察[J];中國血液流變學雜志;2005年03期

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