本文選題：埃博拉病毒　切入點(diǎn)：糖蛋白　出處：《北京協(xié)和醫(yī)學(xué)院》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：埃博拉病毒是一種絲狀病毒科單股負(fù)鏈無節(jié)段的RNA病毒。該病毒可導(dǎo)致埃博拉出血熱,多表現(xiàn)為高熱、休克及出血傾向。在疾病發(fā)展過程中,埃博拉病毒可損害血管內(nèi)皮,進(jìn)而導(dǎo)致血管通透性增加。目前己確定埃博拉病毒的毒性主要源自其表面的糖蛋白(GP)。內(nèi)源性表達(dá)該蛋白可以導(dǎo)致血管內(nèi)皮細(xì)胞失去粘附能力而脫落、漂浮,從而損害血管的完整性。本室建立了外源性攜帶Fc標(biāo)簽的糖蛋白GP1亞基(GP1-Fc)導(dǎo)致血管內(nèi)皮細(xì)胞漂浮的模型。為進(jìn)一步明確該模型中GP1-Fc毒性相關(guān)的結(jié)構(gòu)域,本研究構(gòu)建并表達(dá)、純化了GP1相關(guān)的系列蛋白。同時(shí)發(fā)現(xiàn)了外源性添加的GP1-Fc蛋白以多聚體的形式存在,可能存在模擬天然GP分子構(gòu)象的情況。此外,本研究還證實(shí)了在293T細(xì)胞中內(nèi)源性表達(dá)GP不會(huì)直接導(dǎo)致細(xì)胞死亡。
[Abstract]:Ebola virus is a single-stranded and segmented RNA virus in the family filamentae. The virus can cause Ebola haemorrhagic fever, which is characterized by high fever, shock and haemorrhage tendency. During the development of the disease, Ebola virus can damage the vascular endothelium. This leads to increased vascular permeability. It has been determined that the toxicity of Ebola virus is mainly due to the glycoprotein GPN on its surface. Endogenous expression of this protein can cause vascular endothelial cells to lose adhesion and fall off and float. In order to further clarify the domain associated with GP1-Fc toxicity, we constructed and expressed a model of exogenous glycoprotein GP1 subunit GP1-Fc, which was used to induce vascular endothelial cells to float. A series of proteins related to GP1 were purified. It was also found that exogenous GP1-Fc proteins existed in the form of polymers, which might mimic the conformation of natural GP molecules. This study also confirmed that endogenous expression of GP in 293T cells did not directly lead to cell death.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R373

【共引文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 吳詩坡;基于重組腺病毒載體的烈性病原體疫苗研究[D];中國(guó)人民解放軍軍事醫(yī)學(xué)科學(xué)院;2013年

相關(guān)碩士學(xué)位論文 前2條

1 呂鈞;α屬冠狀病毒S蛋白的變異對(duì)其細(xì)胞感染特異性的影響[D];復(fù)旦大學(xué);2011年

2 宋坤;表達(dá)扎伊爾型埃博拉病毒囊膜糖蛋白重組水泡性口炎病毒的構(gòu)建[D];中國(guó)農(nóng)業(yè)科學(xué)院;2013年

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本文編號(hào)：1557827