本文關鍵詞： 低氧代謝 水通道蛋白-1 水通道蛋白-4 低氧代謝 血管內皮生長因子 可溶性E選擇素　出處：《廣西醫(yī)科大學》2012年碩士論文　論文類型：學位論文

【摘要】：目的：研究肺低氧代謝中大鼠肺組織水通道蛋白1、4(AQP-1、4)的表達,探討低氧代謝與其相關性。 方法：將30只SD大鼠隨機分成對照組和實驗組A、B、C、D組,即1/2h單肺低氧組、1h單肺低氧組、2h單肺低氧組、4h單肺低氧組。對照組采用氣管切開插管行雙肺通氣,實驗組采用氣管切開插管行單肺通氣,建立急性低氧性肺損傷模型。分別于通氣1/2h、1h、2h、4h后取非通氣側肺組織,光鏡下觀察肺組織病理變化情況；免疫組化觀察AQP-1、AQP-4蛋白表達與分布；采用實時熒光定量PCR測定大鼠AQP-1、AQP-4mRNA表達。 結果：光鏡下可見實驗組隨著低氧時間延長,肺組織毛細血管充血,腔內充滿較多紅細胞,肺泡隔增寬,炎性細胞浸潤,而對照組未見明顯病理變化。肺組織免疫組化觀察到隨著低氧時間延長AQP-1表達明顯減少,而AQP-4變化不大。單肺低氧4h組AQP-lmRNA的表達較對照組顯著減少(P0.05),且隨著低氧時間延長呈下降趨勢：ABCD,而AQP-4的表達沒有明顯變化。 結論：長時間低氧代謝導致大鼠肺組織結構發(fā)生改變,AQP-1表達下調,且與低氧代謝時間呈負相關,而AQP-4與肺低氧代謝無相關性。 目的：研究肺低氧代謝中大鼠血管內皮生長因子(VEGF)、可溶性E選擇素(sE-Selectin)的表達,探討低氧代謝與其相關性。 方法：將30只SD大鼠隨機分成對照組和實驗組A、B、C、D組,即1/2h單肺低氧組、1h單肺低氧組、2h單肺低氧組、4h單肺低氧組。對照組采用氣管切開插管行雙肺通氣,實驗組采用氣管切開插管行單肺通氣,建立急性低氧性肺損傷模型。分別于通氣1/2h、1h、2h、4h后取非通氣側大鼠肺組織及血清。光鏡下觀察肺組織病理變化情況,采用ELISA法測定血清中sE-選擇素、肺組織和血清中VEGF的含量,免疫組化觀察VEGF蛋白表達與分布,采用實時熒光定量PCR測定大鼠VEGFmRNA表達。 結果：HE觀察到實驗組隨著低氧時間延長,肺組織毛細血管充血,腔內充滿較多紅細胞,肺泡隔增寬,炎性細胞浸潤,而對照組未見明顯病理變化。ELISA法觀察到sE-選擇素在大鼠單肺低氧1h、2h、4h血清中的表達較對照組顯著升高(P0.05),且隨著時間延長,含量增加；VEGF在大鼠單肺低氧4h組肺組織中的表達較正常組顯著降低(P0.05),而在該組血清中的表達較對照組顯著升高(P0.05)。肺組織免疫組化觀察到隨著低氧時間延長VEGF表達減少。單肺低氧4h組VEGF mRNA的表達較對照組顯著減少(P0.05),且隨著低氧時間延長呈下降趨勢：ABCD。 結論：長時間低氧代謝導致大鼠肺組織結構發(fā)生改變,sE-選擇素表達上調,且與低氧代謝時間呈正相關；而肺組織中VEGF與低氧代謝時間呈負相關。
[Abstract]:Aim: to study the expression of aquaporin 1 (aquaporin 1) in rat lung tissue during hypoxic metabolism, and to explore the correlation between hypoxia metabolism and AQP-4. Methods: thirty Sprague-Dawley rats were randomly divided into two groups: control group and experimental group, namely 1 / 2 h one-lung hypoxia group, 1 / 2 h single lung hypoxia group, 1 h single lung hypoxia group and 4 h single lung hypoxia group, the control group was treated with tracheotomy and intubation to perform double lung ventilation. The model of acute hypoxic lung injury was established by using tracheotomy and intubation to establish acute hypoxic lung injury model. The lung tissues of the non-ventilated side were taken for 4 h after 1 / 2 h of ventilation, and the pathological changes of lung tissue were observed under light microscope, and the expression and distribution of AQP-1AQP-4 protein were observed by immunohistochemistry. The expression of AQP-4 mRNA in rat AQP-1 was measured by real-time fluorescence quantitative PCR. Results: with the prolongation of hypoxic time, pulmonary capillary congestion, more red blood cells, wider alveolar septum and inflammatory cell infiltration were observed under light microscope in the experimental group. In the control group, there was no obvious pathological change. The expression of AQP-1 decreased with the prolongation of hypoxic time by immunohistochemistry. Compared with the control group, the expression of AQP-lmRNA in single lung hypoxia group decreased significantly (P 0.05), and decreased with the increase of hypoxia time, but the expression of AQP-4 did not change significantly. Conclusion: the expression of AQP-1 is down-regulated and negatively correlated with hypoxic metabolic time due to long-term hypoxic metabolism in rats, but AQP-4 has no correlation with hypoxic metabolism. Aim: to study the expression of vascular endothelial growth factor (VEGF) in rat hypoxic metabolism and its correlation. Methods: thirty Sprague-Dawley rats were randomly divided into two groups: control group and experimental group, namely 1 / 2 h one-lung hypoxia group, 1 / 2 h single lung hypoxia group, 1 h single lung hypoxia group and 4 h single lung hypoxia group, the control group was treated with tracheotomy and intubation to perform double lung ventilation. The model of acute hypoxic lung injury was established by tracheotomy and intubation in the experimental group. Lung tissue and serum of the non-ventilated side were taken after 1 / 2 h / 1 h and 2 h / 4 h of ventilation respectively. The pathological changes of lung tissue were observed under light microscope. The content of sE- selectin in serum, VEGF in lung tissue and serum were measured by ELISA method. The expression and distribution of VEGF protein were observed by immunohistochemistry. The expression of VEGFmRNA in rats was measured by real-time fluorescence quantitative PCR. Results with the prolongation of hypoxic time, the blood capillaries of lung tissue in the experimental group were congested, the cavity was filled with more red blood cells, the alveolar septum widened, and the inflammatory cells infiltrated. The expression of sE- selectin in the serum of rats with single lung hypoxia for 1 h or 2 h was significantly higher than that in the control group (P 0.05), and the expression of sE-selectin in the serum of rats with single lung hypoxia for 1 h or 2 h was significantly higher than that in the control group, and the expression of sE-selectin was prolonged with time. The expression of VEGFin in lung tissue of single lung hypoxia group for 4 hours was significantly lower than that in normal group, but the expression in serum of this group was significantly higher than that in control group. The expression of VEGF in lung tissue was observed by immunohistochemistry with the prolongation of hypoxic time. The expression of VEGF mRNA in single lung hypoxia group for 4 h was significantly lower than that in control group (P 0.05), and the expression of VEGF mRNA decreased with the prolongation of hypoxia time. Conclusion: Long-term hypoxic metabolism leads to the changes of lung tissue structure in rats, and the expression of sE- selectin is up-regulated and positively correlated with hypoxic metabolic time, while VEGF is negatively correlated with hypoxic metabolic time.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R363

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