本文關(guān)鍵詞： 細(xì)胞低氧 骨形態(tài)發(fā)生蛋白質(zhì)類 間質(zhì)干細(xì)胞 骨髓 細(xì)胞分化 兔　出處：《脊柱外科雜志》2016年02期 　論文類型：期刊論文

【摘要】：目的研究缺氧狀態(tài)對(duì)骨形態(tài)發(fā)生蛋白-2(BMP-2)誘導(dǎo)分化體外復(fù)合富血小板血漿(PRP)凝膠的骨髓間充質(zhì)干細(xì)胞(BMSCs)的影響。方法取第三代培養(yǎng)的BMSCs細(xì)胞與PRP凝膠相混合,通過(guò)構(gòu)建細(xì)胞缺氧模型,根據(jù)培養(yǎng)條件的不同確定實(shí)驗(yàn)分組:常氧BMSCs+PRP組(A組)、常氧BMP-2+BMSCs+PRP組(B組)、低氧BMSCs+PRP組(C組)、低氧BMP-2+BMSCs+PRP組(D組)。反轉(zhuǎn)錄-聚合酶鏈反應(yīng)法(RT-PCR)及Western-blotting檢測(cè)各組成軟骨及成骨基因的表達(dá)。結(jié)果 RT-PCR檢測(cè)表明C組和D組的Ⅱ型膠原、蛋白聚糖和低氧誘導(dǎo)因子-1α(HIF-1α)表達(dá)均較A組和B組明顯升高;Ⅰ型膠原和堿性磷酸酶(ALP)在B組的表達(dá)最高;A組與B組runt相關(guān)轉(zhuǎn)錄因子2(Runx2)的表達(dá)較C組和D組明顯升高。Western-blotting檢測(cè)結(jié)果表明B組和D組的Ⅱ型膠原表達(dá)較A組和C組明顯升高;C組與D組的HIF-1α表達(dá)較A組與B組顯著升高;D組的SOX-9表達(dá)較其余3組顯著升高。所有結(jié)果差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論低氧條件能顯著促進(jìn)BMP-2對(duì)BMSCs的成軟骨誘導(dǎo)分化,同時(shí)抑制BMSCs的成骨分化。HIF-1α可能是這一過(guò)程的重要調(diào)節(jié)因子,其可能通過(guò)調(diào)節(jié)SOX-9的表達(dá)來(lái)發(fā)揮促BMSCs成軟骨細(xì)胞分化的作用。
[Abstract]:Objective to study the effect of hypoxia on bone morphogenetic protein-2 BMP-2 (BMP-2) -induced differentiation of bone marrow mesenchymal stem cells (BMSCs) combined with platelet-rich plasma (PRP) gel in vitro. Methods BMSCs cells cultured in the third generation were mixed with PRP gel. According to the different culture conditions, the cells were divided into two groups: normoxic BMSCs PRP group (group A), normoxic BMP-2 BMSCs group (group B) and normoxic BMP-2 BMSCs group (group B). Hypoxia BMSCs PRP group (group C). Reverse transcription-polymerase chain reaction (RT-PCR) in hypoxic BMP-2 BMSCs PRP group. Results the expression of osteogenic genes in cartilage and osteogenesis was detected by Western-blotting. Results the type 鈪，

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