本文關(guān)鍵詞： 卵白蛋白 變應(yīng)性鼻炎 WHBE兔 樹突狀細(xì)胞　出處：《中國實(shí)驗(yàn)動(dòng)物學(xué)報(bào)》2017年03期 　論文類型：期刊論文

【摘要】：目的比較觀察卵白蛋白(OVA)誘導(dǎo)的白毛黑眼兔(WHBE兔)和日本大耳白兔(JW兔)變應(yīng)性鼻炎(allergic rhinitis,AR)模型外周血來源樹突狀細(xì)胞(dendritic cells,DC)的功能,揭示W(wǎng)HBE兔對(duì)AR敏感的可能機(jī)制。方法以O(shè)VA誘導(dǎo)建立WHBE兔和JW兔AR模型,每次激發(fā)后觀察動(dòng)物一般體征,進(jìn)行鼻黏膜HE染色,觀察組織病理學(xué)改變。分離外周血DC,流式細(xì)胞儀檢測(cè)外周血DC表面分子CD86的表達(dá)以及DC攝取抗原的能力,同時(shí),采用熒光定量PCR檢測(cè)外周血DC甘露糖受體(mannose receptor,MR)的mRNA表達(dá)水平。進(jìn)一步以CFSE標(biāo)記T細(xì)胞,流式細(xì)胞儀檢測(cè)外周血DC誘導(dǎo)T細(xì)胞增殖能力。結(jié)果一般體征和HE染色觀察顯示,WHBE兔和JW兔模型組動(dòng)物均表現(xiàn)為典型的變應(yīng)性鼻炎癥狀和組織病理學(xué)改變,提示模型構(gòu)建成功。WHBE兔AR模型組外周血DC CD86的表達(dá)不僅極顯著高于正常對(duì)照組,亦極顯著高于JW兔AR模型組(P0.01,P0.01)。正常穩(wěn)態(tài)下,WHBE兔外周血DC MR的mRNA相對(duì)表達(dá)量極顯著高于JW兔(P0.01),經(jīng)OVA誘發(fā)建立AR模型,WHBE兔外周血DC MR的mRNA表達(dá)水平顯著升高(P0.05),且顯著高于JW兔模型組(P0.05)。并且,WHBE兔模型組外周血DC攝取OVA647的能力極顯著高于正常對(duì)照組(P0.01),亦極顯著高于JW兔模型組(P0.01)。結(jié)論 WHBE兔DC對(duì)變應(yīng)原更敏感,可能與其高表達(dá)抗原識(shí)別受體MR,具備更強(qiáng)的分化成熟、攝取抗原能力有關(guān)。
[Abstract]:Objective to observe the allergic rhinitis induced by ovalbumin (OVA) in white haired black eyed rabbits (WHBE) and Japanese large ear white rabbits (JW). The function of dendritic cells derived from peripheral blood derived from dendritic cells (DC). Methods the AR models of WHBE rabbits and JW rabbits were induced by OVA. The general signs of the animals were observed and nasal mucosa HE staining was performed after each stimulation. The changes of histopathology were observed. The expression of CD86 on the surface of peripheral blood DC and the ability of DC to absorb antigen were detected by flow cytometry. The expression of mRNA in peripheral blood DC mannose receptor MRs was detected by fluorescence quantitative PCR, and T cells were further labeled with CFSE. The proliferation ability of T cells induced by peripheral blood DC was detected by flow cytometry. Results the general signs and HE staining were observed. WHBE rabbits and JW rabbits showed typical allergic rhinitis symptoms and histopathological changes. The results suggested that the expression of DC CD86 in the model group was significantly higher than that in the normal control group and JW rabbit AR model group (P0.01). The relative expression of mRNA in peripheral blood DC Mr was significantly higher in white rabbits than that in JW rabbits under normal steady-state condition. The AR model was induced by OVA. The mRNA expression of DC Mr in peripheral blood of WHBE rabbits was significantly higher than that of JW rabbit model group. The ability of DC uptake of OVA647 in WHBE rabbit model group was significantly higher than that in normal control group (P0.01). Conclusion WHBE rabbit DC is more sensitive to allergen than JW rabbit model group, and may have stronger differentiation and maturity with high expression of antigen recognition receptor MRR. The ability to ingest antigen is related.
【作者單位】： 浙江中醫(yī)藥大學(xué)動(dòng)物實(shí)驗(yàn)研究中心/比較醫(yī)學(xué)研究所;浙江中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院;
【基金】：浙江省科技計(jì)劃項(xiàng)目(批準(zhǔn)號(hào):2014C37007) 浙江中醫(yī)藥大學(xué)比較醫(yī)學(xué)創(chuàng)新團(tuán)隊(duì)(XTD201301)
【分類號(hào)】：R-332;R765.21
【正文快照】： ges.Expressions of CD86 on the cell surface of dendritic cells in WHBE rabbits with AR were significantly upregulated notonly compared with the normal control(NC)rabbits,but also with the JW rabbits with AR(P0.01).The result of real-time PCR assay showe

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 鄭義;龔輝;李凡成;;變應(yīng)性鼻炎豚鼠造模綜述[J];醫(yī)學(xué)信息(中旬刊);2011年06期

2 張麗;杜經(jīng)緯;王麗洪;譚毅;龔曉波;江鵬;王智彪;;甲苯-2,4-二異氰酸酯經(jīng)鼻致敏建立羊變應(yīng)性鼻炎模型[J];中國實(shí)驗(yàn)動(dòng)物學(xué)報(bào);2009年02期

3 馮緯紜;謝芳;;變應(yīng)性鼻炎動(dòng)物模型造模綜述[J];廣西中醫(yī)學(xué)院學(xué)報(bào);2009年02期

4 張麗;杜經(jīng)緯;王麗洪;譚毅;龔曉波;江鵬;王智彪;;豚鼠和新西蘭兔變應(yīng)性鼻炎模型建立及比較[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2010年03期

5 張華;趙娟;雍軍;孫捷;;實(shí)驗(yàn)性變應(yīng)性鼻炎鼠類模型的建立[J];新疆醫(yī)科大學(xué)學(xué)報(bào);2006年01期

6 羅燦嶠;莫木瓊;鐘覺民;廖美芳;;變應(yīng)性鼻炎大鼠模型的建立方法[J];實(shí)用醫(yī)技雜志;2008年29期

7 邸嬋娟;李海兵;韓春光;胡明;原美茹;劉永學(xué);;卵白蛋白誘發(fā)變應(yīng)性鼻炎豚鼠模型的特點(diǎn)及鑒定[J];癌變·畸變·突變;2012年06期

8 劉建國;楊政;劉月輝;;變應(yīng)性鼻炎大鼠模型建造[J];江西醫(yī)學(xué)院學(xué)報(bào);2008年05期

9 徐孝平;徐劍欽;陳民利;陳方明;陳亮;彭定國;周衛(wèi)民;;兔變應(yīng)性鼻炎模型的建立及其鼻黏膜組織學(xué)觀察[J];實(shí)驗(yàn)動(dòng)物與比較醫(yī)學(xué);2006年02期

10 邱寶珊,王士貞,梁曉陽,阮巖;腎陽虛型變應(yīng)性鼻炎動(dòng)物模型的建立[J];中國中西醫(yī)結(jié)合耳鼻咽喉科雜志;2002年01期

相關(guān)會(huì)議論文 前3條

1 邸嬋娟;李海兵;胡明;韓春光;原美茹;劉永學(xué);;變應(yīng)性鼻炎動(dòng)物模型的誘發(fā)[A];第十四屆中國科協(xié)年會(huì)第17分會(huì)場(chǎng)：環(huán)境危害與健康防護(hù)研討會(huì)論文集[C];2012年

2 鄭義;龔輝;李凡成;;變應(yīng)性鼻炎豚鼠造模綜述[A];世界中聯(lián)耳鼻喉口腔專業(yè)委員會(huì)換屆大會(huì)及第三次學(xué)術(shù)年會(huì)暨中華中醫(yī)藥學(xué)會(huì)耳鼻喉科分會(huì)第十七次學(xué)術(shù)交流會(huì)暨廣東省中醫(yī)及中西醫(yī)結(jié)合學(xué)會(huì)耳鼻喉科學(xué)術(shù)交流會(huì)論文匯編[C];2011年

3 李海兵;邸嬋娟;王敏;胡明;劉永學(xué);;TDI誘發(fā)變應(yīng)性鼻炎豚鼠模型的建立及特點(diǎn)[A];低碳生活與健康損害論壇——中國環(huán)境誘變劑學(xué)會(huì)風(fēng)險(xiǎn)評(píng)價(jià)專業(yè)委員會(huì)全國第十三屆學(xué)術(shù)會(huì)議暨第四屆第5次委員會(huì)會(huì)議論文集[C];2011年

相關(guān)碩士學(xué)位論文 前9條

1 萬慧娟;NLRP3炎性小體及下游因子IL-1β/IL-18在變應(yīng)性鼻炎大鼠模型中的表達(dá)及意義[D];鄭州大學(xué);2015年

2 趙冬菊;小鼠變應(yīng)性鼻炎最輕持續(xù)炎癥狀態(tài)模型的制作[D];上海交通大學(xué);2014年

3 寧娜;槲皮素治療變應(yīng)性鼻炎模型小鼠的效果及其機(jī)制探討[D];華中科技大學(xué);2013年

4 李蕓;鼻敏爽膠囊對(duì)變應(yīng)性鼻炎豚鼠模型Th1/Th2細(xì)胞因子表達(dá)的影響[D];云南中醫(yī)學(xué)院;2013年

5 林曾萍;變應(yīng)性鼻炎小鼠模型建立及鼻粘膜水通道蛋白5的表達(dá)及意義[D];遵義醫(yī)學(xué)院;2014年

6 熊雯雯;變應(yīng)性鼻炎模型中NF-κB和iNOS的表達(dá)及意義[D];南華大學(xué);2014年

7 蘇磊;鹽酸賽洛唑啉聯(lián)合鼻用糖皮質(zhì)激素對(duì)變應(yīng)性鼻炎模型大鼠鼻黏膜組織中ICAM-1、IL-12表達(dá)的影響[D];鄭州大學(xué);2014年

8 李冠雪;脂肪來源間充質(zhì)干細(xì)胞（ADSC）調(diào)控變應(yīng)性鼻炎模型鼠T細(xì)胞免疫狀態(tài)的研究[D];南方醫(yī)科大學(xué);2013年

9 劉文軍;MMP-9、TNF-α表達(dá)與上下呼吸道炎癥反應(yīng)一致性[D];瀘州醫(yī)學(xué)院;2010年

，

本文編號(hào)：1469322