本文選題：腎陽虛便秘 + 濟川煎��； 參考：《山西省中醫(yī)藥研究院》2017年碩士論文

【摘要】：目的運用活性炭聯(lián)合冰水和5%乙酸法,制造大鼠腎陽虛便秘動物模型,探究溫陽通便法治療腎陽虛便秘與胃動素(MTL)、P物質(SP)和生長抑素(SS)水平以及與cAMP、cGMP、T3、T4的關系。方法60只SD大鼠隨機分為5組,即空白組,模型組,濟川煎高、中、低劑量組,每組12只,把濟川煎高(3.8g/kg)、中(1.9g/kg)、低(0.95g/kg)劑量組,分別配制成25.2、12.6、6.3%的濃度,5%乙酸合并活性炭冰水造模4周后,各組按1.5ml/100g灌胃給藥,模型組給予等體積生理鹽水,每天1次,連續(xù)7天。觀察大鼠的一般生理變化,記錄大鼠首粒排便時間及其6小時內排便數(shù)量,以及大鼠結腸的肌電和HE染色病理切片,分別用Elisa法和免疫組化法(SABC法)檢測大鼠血清和結腸中MTL、SP和SS水平等實驗,并用Elisa法測定大鼠血清cAMP、cGMP、T3、T4含量。結果結腸病理切片各實驗組均未見差異。造模后大鼠一般生理學特征為:腹脹、披毛變硬、身體蜷縮、體重變輕、大便色黑干燥。與空白組對比,模型組的大鼠排便時間延長,排便粒數(shù)減少,胃腸蠕動減弱,血清和結腸中MTL水平降低,P物質不變,SS含量升高。治療組的上述體征和指標有明顯改善,其中濟川煎高、中劑量組與濟川煎低劑量組比較,療效更明顯。此外還觀察到了濟川煎能夠明顯糾正腎陽虛便秘模型動物cAMP、cGMP失衡的作用,但本模型T3、T4無明顯變化。結論活性炭聯(lián)合冰水和5%乙酸法造模后,結腸沒有發(fā)生器質性病變,屬于功能性便秘,大鼠各體征表現(xiàn)符合腎陽虛證候,所造出的大鼠便秘模型是腎陽虛便秘模型。濟川煎治療腎陽虛便秘機理與提高MTL水平,降低SS水平有密切關系,與P物質關系不大,且濟川煎高、中劑量組治療腎陽虛便秘效果更加顯著,濟川煎還通過糾正本模型動物cAMP、cGMP的失衡而發(fā)揮改善腎陽虛的功能,體現(xiàn)了標本兼治的作用特點。
[Abstract]:Objective to establish an animal model of kidney-yang deficiency constipation in rats by using activated carbon combined with ice water and 5% acetic acid, and to explore the relationship between the treatment of kidney-yang deficiency constipation and motilin motilin (motilin) and somatostatin (SS), and the relationship between them and cAMPG MPT _ (3) T _ (3) T _ (4). Methods Sixty Sprague-Dawley rats were randomly divided into 5 groups: blank group, model group, Jichuan decoction high, middle and low dose groups, 12 rats in each group. The rats were divided into 3. 8 g / kg, 1. 9 g / kg, 0. 95 g / kg and 0. 95 g / kg, respectively. After 4 weeks of modeling with 5% acetic acid and activated carbon ice water, each group was given intragastric administration of 1.5ml/100g, and the model group was given normal saline of equal volume once a day for 7 days. To observe the general physiological changes of rats, to record the first defecation time and the number of defecation within 6 hours, and to observe the myoelectric and HE staining pathological sections of rat colon. The levels of MTLS-SP and SS in serum and colon of rats were detected by Elisa method and immunohistochemical method, respectively. The content of cAMP-cGMP-T3T3T4 in serum of rats was measured by Elisa method. Results there was no difference in colonic pathological section among the experimental groups. The general physiological characteristics of the model rats were abdominal distension, drape, body curling, light weight, dry stool color and black. Compared with the blank group, the defecation time of the model group was prolonged, the defecation granule number was decreased, the gastrointestinal peristalsis was weakened, the MTL level in serum and colon was decreased and the content of SS was increased. The above signs and indexes in the treatment group were obviously improved, among them, the effect of Jichuan decoction was higher than that of the middle dose group and the Jichuan decoction low dose group. In addition, it was observed that Jichuan decoction could obviously correct the imbalance of cAMPN cGMP in animal model of kidney yang deficiency constipation, but there was no obvious change of T3T4 in this model. Conclusion the model of activated carbon combined with ice water and 5% acetic acid has no organic lesion and belongs to functional constipation. The symptoms of each sign of rats accord with the syndrome of deficiency of kidney yang. The model of constipation in rats is the model of constipation due to deficiency of kidney yang. The mechanism of Jichuan decoction in treating constipation of kidney-yang deficiency was closely related to raising MTL level and decreasing SS level, but had little to do with substance P, and Jichuan decoction had a higher effect on constipation of kidney-yang deficiency, and the effect of medium dose group on constipation of kidney-yang deficiency was more remarkable. Jichuan decoction also improved the function of kidney yang deficiency by correcting the imbalance of cAMP and cGMP in the model animal, and reflected the function of both symptoms and symptoms.
【學位授予單位】：山西省中醫(yī)藥研究院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R256.35

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