本文選題：血管性癡呆　切入點(diǎn)：線粒體　出處：《吉林大學(xué)》2017年碩士論文

【摘要】：目的:探討人參皂苷Re對(duì)慢性缺血性血管癡呆(VD)大鼠海馬神經(jīng)元缺血損傷和線粒體功能障礙的保護(hù)作用,為開發(fā)安全有效的防治VD藥物提供實(shí)驗(yàn)依據(jù)。方法:應(yīng)用分次離斷結(jié)扎雙側(cè)頸總動(dòng)脈法建立VD大鼠模型;利用Morris水迷宮和HE染色判定模型構(gòu)建效果;采用試劑盒提取各組線粒體及蛋白質(zhì),并通過電鏡技術(shù)、Western-blot實(shí)驗(yàn)和H_2O_2試劑盒比較各組差異。利用真空泵和CO_2孵箱建立缺氧復(fù)氧模型,采用LDH釋放量檢測(cè)、DNA瓊脂糖電泳及細(xì)胞形態(tài)觀察驗(yàn)證模型建立效果。進(jìn)一步通過檢測(cè)各組LDH釋放量、DNA斷裂程度及細(xì)胞狀態(tài),并通過提取線粒體和蛋白質(zhì)和應(yīng)用Western Blot和H_2O_2試劑盒研究人參皂苷Re的作用。結(jié)果:分次離斷結(jié)扎大鼠雙側(cè)頸總動(dòng)脈后,大鼠認(rèn)知能力下降,逃避潛伏期明顯延長(zhǎng)。細(xì)胞形態(tài)結(jié)構(gòu)顯示,大鼠海馬神經(jīng)元排列紊亂,細(xì)胞周圍呈空泡化,失去正常結(jié)構(gòu)。COX IV、PDH-A1和H_2O_2的表達(dá)結(jié)果顯示,不同濃度的人參皂苷Re對(duì)大鼠VD線粒體的COX IV和PDH-A1的表達(dá)均有明顯促進(jìn)作用,而對(duì)H_2O_2釋放量有一定抑制作用,表明人參皂苷Re可修復(fù)海馬神經(jīng)元的線粒體損傷。大鼠海馬神經(jīng)元經(jīng)缺氧復(fù)氧后,其模型組LDH釋放量增高、DNA斷裂加劇、神經(jīng)元突起退化及胞體固縮;而人參皂苷Re可有效抑制LDH釋放、DNA斷裂及突觸退化,促進(jìn)對(duì)線粒體COX IV和PDH-A1的表達(dá),抑制H_2O_2的釋放。結(jié)論:人參皂苷Re可對(duì)腦缺血所造成的海馬神經(jīng)元線粒體損傷起保護(hù)作用。人參皂苷Re可通過改善海馬神經(jīng)元的線粒體損傷,改善大鼠的認(rèn)知能力。
[Abstract]:Objective: to investigate the protective effect of ginsenoside re on hippocampal neuronal ischemia injury and mitochondrial dysfunction in rats with chronic ischemic vascular dementia (VD), and to provide experimental evidence for the development of safe and effective drugs for the prevention and treatment of VD.Methods: VD rat model was established by severing and ligating bilateral common carotid artery, Morris water maze and HE staining were used to determine the effect of model construction, mitochondria and protein were extracted from each group by kit.Western blot and H_2O_2 kit were used to compare the differences.The model of anoxia and reoxygenation was established by vacuum pump and CO_2 incubator. The effect of the model was verified by using LDH release quantity to detect agarose electrophoresis and observe cell morphology.The effect of ginsenoside re on the activity of ginsenoside re was studied by detecting the amount of LDH released in each group and the cell state, and by extracting mitochondria and proteins, and using Western Blot and H_2O_2 kit to study the effect of ginsenoside re.Results: the cognitive ability of bilateral common carotid artery was decreased and the escape latency was prolonged.The morphological structure of the cells showed that the hippocampal neurons in the rats were disordered and vacuolated around the cells, and the expression of PDH-A1 and H_2O_2 were found to be lost in the normal structure.Different concentrations of ginsenoside re could obviously promote the expression of COX IV and PDH-A1 in VD mitochondria, but inhibit the release of H_2O_2, suggesting that ginsenoside re could repair the mitochondrial damage of hippocampal neurons.After hypoxia and reoxygenation, the release of LDH in the model group increased, the neurite degeneration and cell body pyknosis were aggravated, while ginsenoside re could effectively inhibit the LDH release and synaptic degeneration.Promote the expression of mitochondrial COX IV and PDH-A1 and inhibit the release of H_2O_2.Conclusion: ginsenoside re can protect hippocampal neuron mitochondria from cerebral ischemia.Ginsenoside re can improve the cognitive ability of rats by improving mitochondria damage of hippocampal neurons.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R285.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 陳美華;吳月鵬;張顏波;金毅;;人參皂苷Rg2對(duì)腦缺血再灌注大鼠神經(jīng)保護(hù)作用的研究[J];中風(fēng)與神經(jīng)疾病雜志;2014年12期

2 張振宇;侯祥紅;孟姍姍;李丹;張向楠;李彬;王基野;柯濤;張文斌;駱文靜;;丙酮酸對(duì)細(xì)胞寒冷應(yīng)激損傷的保護(hù)作用研究[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2013年20期

3 王洪財(cái);蔣玉萌;趙雪;王寧;高迪;關(guān)銘;張?bào)@宇;楊子超;;人參皂苷Rb1對(duì)Aβ淀粉樣蛋白誘導(dǎo)大鼠海馬神經(jīng)元損傷的保護(hù)作用[J];吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2012年03期

4 ;Hippocampal mitochondrial cytochrome C oxidase activity and gene expression in a rat model of chronic cerebral ischemia[J];Neural Regeneration Research;2011年32期

，

本文編號(hào)：1719765