【摘要】：睡蓮花Nymphaea candida Presl,維吾爾醫(yī)稱為“內(nèi)魯帕爾”,是雪白睡蓮的干燥花蕾,該藥材具有清熱解毒、益心護(hù)腦、鎮(zhèn)靜安神等功效,在維醫(yī)臨床中主要用于熱癥引起的頭疼、熱感咳嗽及小兒急、慢驚風(fēng)等病癥的治療。在前期研究基礎(chǔ)上,本文對(duì)睡蓮花總黃酮有效部位進(jìn)行了以下幾方面的研究。(1)通過高速逆流色譜對(duì)睡蓮花標(biāo)識(shí)性成分進(jìn)行了分離純化,采用乙酸乙酯-甲醇-水=(4:1:5 v/v)和乙酸乙酯-正丁醇-水=(4:1:5 v/v)作為溶劑體系,通過兩步分離純化得到了異槲皮苷、紫云英苷、isostrictiniin和煙花苷,它們的純度分別為73.53%、92.42%、82.36%和85.65%。(2)以煙花苷、紫云英苷含量為考察指標(biāo),采用單因素考察結(jié)合正交試驗(yàn)優(yōu)選出睡蓮花總黃酮的最佳提取工藝為:提取溶劑選為70%的乙醇,料液比選為1:10、回流提取2次,每次提取時(shí)間為1.0 h。(3)以煙花苷、紫云英苷含量為考察指標(biāo),采用單因素考察優(yōu)選出了睡蓮花總黃酮的最佳純化工藝:大孔樹脂最佳上樣濃度為煙花苷0.3220 mg/L、紫云英苷0.0865 mg/L,5BV 30%乙醇除雜,5 BV 50%乙醇洗脫;大孔樹脂初步純化后,再用聚酰胺吸附樹脂進(jìn)行富集純化,其工藝條件為:樣品與聚酰胺的比例為1:8,10 BV水除雜,5 BV 70%乙醇洗脫。(4)建立了睡蓮花總黃酮的理化鑒別、薄層色譜鑒別方法;依據(jù)《中國藥典》2015版第四部,測(cè)定了睡蓮花總黃酮的水分、熾灼殘?jiān)⒅亟饘俸蜕辂},初步限定含水分不得超過7.0%,熾灼殘?jiān)坏眠^4.0%,重金屬不得過百萬分之二十,砷鹽不得過百萬分之零點(diǎn)五。通過高溫、高濕和強(qiáng)光等影響因素試驗(yàn)對(duì)睡蓮花總黃酮進(jìn)行初步穩(wěn)定性研究,結(jié)果表明睡蓮花總黃酮外觀、色澤及總黃酮標(biāo)示性成分無明顯影響,性質(zhì)穩(wěn)定。建立了HPLC法測(cè)定睡蓮花總黃酮標(biāo)識(shí)性成分含量測(cè)定的方法。建立了分光光度法測(cè)定睡蓮花總黃酮含量的方法。
[Abstract]:Water lily flower Nymphaea candida Presl, Uygur medicine is called "Nerupar", is the dry flower bud of white water lily. It has the functions of clearing heat and detoxifying, benefiting heart and protecting brain, calming and calming the mind, etc. It is mainly used for headache caused by hyperthermia in Uygur medicine. The treatment of fever cough and acute and slow shock in children. On the basis of previous studies, the effective components of total flavonoids in water lily flower were studied in the following aspects. (1) the identifiable components of water lily flower were separated and purified by high speed countercurrent chromatography. Using ethyl acetate, methanol-water = (4:1:5 v / v) and ethyl acetate / n-butanol-water = (4:1:5 v / v) as solvent system, isostrictiniin and pyroginoside were separated and purified by two steps. Their purity were 73.53% 92.4282.36% and 85.65% respectively. (2) the purity of isostrictiniin was 73.53%. The optimum extraction technology of total flavonoids of water lily flower was selected by single factor investigation combined with orthogonal test. The optimum extraction process was as follows: the solvent was selected as 70% ethanol, the ratio of material to liquid was 1: 10, and the reflux extraction was 2 times. The extraction time was 1.0 h. (3) the contents of fireworks and porphyrin were used as the index. The optimum purification process of total flavonoids from water lily flower was investigated by single factor. The best concentration of macroporous resin was 0.3220 mg/L, 0.0865 mg/L,5BV 30% ethanol, 5 BV 50% ethanol elution, and after the primary purification of macroporous resin, the optimum concentration of macroporous resin was 0.3220 mg/L,5BV, 30% ethanol and 50% ethanol, respectively. Then the polyamide adsorbent resin was used for enrichment and purification. The technological conditions were as follows: the ratio of sample to polyamide was 1: 8 ~ (10) BV water removal was 5 BV 70% ethanol elution. (4) Physicochemical identification and TLC identification of total flavonoids of water lily flower were established. According to the fourth part of the 2015 edition of the Chinese Pharmacopoeia, the water content, incandescent residue, heavy metal and arsenic salt of the total flavonoids of water lily flower were determined. The preliminary limit was that the moisture content should not exceed 7.0, the incandescent residue should not exceed 4.0, and the heavy metal should not exceed 20/1000000. Arsenic should not exceed 0.5/1000000. The effects of high temperature, high humidity and strong light on the stability of total flavonoids in water lily flower were studied. The results showed that the appearance, color and marked composition of total flavonoids in water lily flower were not significantly affected, but the properties were stable. A HPLC method for the determination of total flavonoids in water lily was established. A spectrophotometric method for the determination of total flavonoids in water lily flower was established.
【學(xué)位授予單位】：新疆大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TQ464

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