本文選題：羅丹明B + 拉曼散射　； 參考：《湖北工業(yè)大學(xué)》2017年碩士論文

【摘要】：隨著人民生活水平的提高,人們?cè)絹?lái)越關(guān)心食品的安全,食品安全始終是社會(huì)關(guān)注的重大問(wèn)題,而食品中各種違法添加物是健康食品的重大威脅之一。拉曼散射檢測(cè)技術(shù)大多不接觸試樣,無(wú)需對(duì)試樣進(jìn)行預(yù)處理,具有快速、準(zhǔn)確的優(yōu)點(diǎn)。本文以違法添加物羅丹明B為研究對(duì)象,運(yùn)用拉曼散射檢測(cè)技術(shù)實(shí)現(xiàn)了對(duì)羅丹明B拉曼圖譜的獲取和實(shí)驗(yàn)數(shù)據(jù)處理,論文的主要內(nèi)容如下:1、基于表面加強(qiáng)拉曼信號(hào)技術(shù),對(duì)羅丹明B拉曼信號(hào)加強(qiáng)方法進(jìn)行了實(shí)驗(yàn)研究。對(duì)比了納米Ag增強(qiáng)劑和Au增強(qiáng)劑對(duì)羅丹明B拉曼信號(hào)的增強(qiáng)效果,以及Ag增強(qiáng)劑中匹配劑的作用。在此基礎(chǔ)上,探討了Ag增強(qiáng)劑,羅丹明B水溶液,匹配劑添加體積比例對(duì)拉曼信號(hào)增強(qiáng)效果的影響。結(jié)果表明,在785nm激光激發(fā)下,Ag增強(qiáng)劑對(duì)羅丹明B拉曼信號(hào)增強(qiáng)效果更佳,匹配劑的加入可顯著提高增強(qiáng)效果;當(dāng)羅丹明B溶液濃度為0.7ppm時(shí),當(dāng)Ag增強(qiáng)劑:羅丹明B水溶液:匹配劑添加體積比例為1:4:1時(shí),羅丹明B拉曼信號(hào)強(qiáng)度最大。2、在拉曼信號(hào)平滑去噪中,利用小波變換和窗口滑動(dòng)平均法對(duì)羅丹明B拉曼信號(hào)進(jìn)行了去噪,對(duì)比RSN和RMSE,小波變換硬閾值化法平滑去噪作用更好。在拉曼光譜基線消除中,在分段多項(xiàng)式擬合基線的基礎(chǔ)上,創(chuàng)新利用了分段三次樣條擬合基線的有效方法。三次樣條函數(shù)其特點(diǎn)是一、二階導(dǎo)數(shù)連續(xù)可導(dǎo),則其刻畫出的曲線連接過(guò)渡平滑,用其擬合光譜基線,能改善信號(hào)的信噪比。通過(guò)對(duì)比最小二乘法擬合基線方法,其擬合出的基線不僅平滑而且準(zhǔn)確,對(duì)羅丹明B的光譜基線消除效果更好。3、利用峰面積來(lái)計(jì)算拉曼特征峰光譜強(qiáng)度,并基于Wasson峰面積計(jì)算公式提出了累加峰強(qiáng)值法快速計(jì)算拉曼特征峰強(qiáng)度。針對(duì)拉曼光譜定量分析方法,結(jié)合外標(biāo)法研究了多變量線性回歸方法,通過(guò)主成分線性回歸和偏最小二乘法線性回歸完成了對(duì)羅丹明B拉曼數(shù)據(jù)的簡(jiǎn)單定量分析。
[Abstract]:With the improvement of people's living standards, people are more and more concerned about the safety of food. Food safety has always been a major concern of the society, and various illegal additives in food is one of the major threats to healthy food. Most Raman scattering techniques do not contact the sample and do not need to be pretreated. It has the advantages of rapidity and accuracy. In this paper, the illegal additive Rhodamine B is used as the research object, and the Raman spectrum of Rhodamine B is obtained and the experimental data are processed by Raman scattering technique. The main contents of this paper are as follows: 1, based on surface-enhanced Raman signal technology. The enhancement method of rodamine B Raman signal was studied experimentally. The effects of nano-Ag reinforcements and au reinforcements on the Raman signal enhancement of Rhodamine B and the matching agents in Ag reinforcements were compared. On this basis, the effects of Ag reinforcer, Rhodamine B aqueous solution and the proportion of matching agent on the enhancement of Raman signal were discussed. The results show that the enhancement effect of Ag on the Raman signal of Rhodamine B is better under the excitation of 785nm laser, and the enhancement effect can be improved by adding the matching agent, and when the concentration of Rhodamine B solution is 0.7ppm, the enhancement effect is better when the concentration of Rhodamine B solution is 0.7ppm. When Ag reinforcer: Rhodamine B aqueous solution: matching agent added volume ratio is 1:4:1, the intensity of Rhodamine B Raman signal is maximum. The rodamine B Raman signal is de-noised by wavelet transform and window moving average method. Compared with RSN and RMSE, wavelet transform hard threshold method is better for smooth denoising. On the basis of piecewise polynomial fitting baselines, the effective method of piecewise cubic spline fitting baselines is innovated in the elimination of Raman spectral baselines. The cubic spline function is characterized by continuous derivation of first and second derivative, then the curve is smooth and the signal to noise ratio can be improved by fitting the spectral baseline with the cubic spline function. By comparing the least square method to fit the baseline, the fitted baseline is not only smooth and accurate, but also can eliminate the spectral baseline of Rhodamine B. the spectral intensity of Raman characteristic peak is calculated by using peak area. Based on the formula of Wasson's peak area, the accumulative peak intensity method is proposed to calculate the Raman peak intensity quickly. In this paper, the multivariate linear regression method is studied based on the quantitative analysis of Raman spectroscopy and the external standard method. The simple quantitative analysis of rhodamine B Raman data is accomplished by principal component linear regression and partial least square linear regression.
【學(xué)位授予單位】：湖北工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TS207.3;O657.37

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 高嘉敏;張卓e，

本文編號(hào)：2028712