The efficiency of plant cytidine base-editing systems is limited, and unwanted mutations frequently occur in transgenic plants. We increased the cytidine editing frequency and fidelity of the plant base editor 3（BE3） and targeted activation-induced cytidine deaminase（CDA）（target-AID） systems by coexpressing three copies of free uracil–DNA glycosylase（UDG） inhibitor（UGI）. The editing efficiency of the improved BE3 and CDA systems reached as high as 88.9% and 85.7%, respectively, in regenerated ri... 

【文章來源】：The Crop Journal. 2020,8(03)SCICSCD

【文章頁數(shù)】：7 頁

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. Vector construction
    2.2. Rice transformation
    2.3. DNA extraction and genotyping
3. Results
4. Discussion
Declaration of Competing Interest


【參考文獻(xiàn)】：
期刊論文
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本文編號(hào)：3209524