本文選題：消化后AB-PAS　切入點(diǎn)：黏液染色　出處：《臨床與實(shí)驗(yàn)病理學(xué)雜志》2017年07期 　論文類型：期刊論文

【摘要】：正AB-PAS是一種常見的特殊染色法,其能較好地顯示中性黏液和酸性黏液,但有時(shí)組織內(nèi)含有的糖原也會(huì)顯示,且無法區(qū)分中性黏液,從而影響染色結(jié)果的判斷。本科室根據(jù)糖原可被淀粉酶分解破壞的原理~([1]),在染色前用淀粉酶消化切片中的糖原,再進(jìn)行A B-PAS染色,取得良好的效果,現(xiàn)介紹如下。1材料與方法1.1標(biāo)本來源收集深圳市人民醫(yī)院病理科已知AB-PAS染色陽性標(biāo)本,每份標(biāo)本石蠟切片2張,厚4μm,分為兩組:(1)常規(guī)AB-PAS染色(對(duì)照組),(2)消化后行AB-PAS染色(實(shí)驗(yàn)組)。
[Abstract]:Positive AB-PAS is a special staining method, which can display neutral mucus and acidic mucus well, but sometimes the glycogen in tissues also shows, and can not distinguish neutral mucus. According to the principle that glycogen can be destroyed by amylase, the glycogen was digested by amylase before dyeing, and then stained with AB-PAS. This paper introduces the following 1. 1 materials and methods 1. 1 specimens collected from the Department of Pathology of Shenzhen people's Hospital, 2 paraffin sections per specimen, known to be positive for AB-PAS staining. Four 渭 m thick, divided into two groups: 1) routine AB-PAS staining (control group 2) and AB-PAS staining after digestion (experimental group).
【作者單位】： 深圳市人民醫(yī)院病理科;廣州軍區(qū)廣州總醫(yī)院病理科;
【分類號(hào)】：R361.2

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1 左汝鐸;;淋巴結(jié)的石蠟制片技術(shù)及常用特殊染色法和組織化學(xué)[J];湖北醫(yī)學(xué)院學(xué)報(bào);1984年03期

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