胰腺癌差異蛋白質組學研究
發(fā)布時間：2018-11-05 14:38
【摘要】：目的通過差異蛋白質組學研究,確定胰腺癌組織及相應正常胰腺組織之間蛋白質表達譜的差異,進而闡明其表達水平的變化與胰腺癌發(fā)生發(fā)展之間的關系。方法選取胰腺癌組織及相應正常胰腺組織6對,提取總蛋白進行雙向聚丙烯酰胺凝膠電泳,經PDQuest軟件分析,獲得差異表達蛋白質點。差異蛋白質點經過切取、酶解后進行電噴霧四極桿飛行時間質譜(ESI-Q-TOF MS/MS)鑒定。質譜數(shù)據使用MASCOT搜索引擎對ExPASy蛋白質序列數(shù)據庫進行檢索,即獲得差異表達的蛋白質。對鑒定出的部分蛋白質通過免疫組化方法進行驗證。結果經雙向凝膠電泳分析共有97個點被確定為差異表達,其中31個點已被成功鑒定,23個蛋白上調,8個蛋白下調。這些蛋白質涉及體內不同的功能過程和蛋白家族,主要包括:糖酵解相關酶,透明質酸酶,Ⅰ、Ⅱ相代謝酶,金屬蛋白酶,細胞骨架,Ca2+代謝相關酶等。免疫組化方法進一步證實了HYAL1表達上調,CYP2C8和GSTM2表達下調。結論應用比較蛋白質組學方法可篩選出胰腺癌患者癌組織和正常胰腺組織間存在的差異表達蛋白,篩選出的蛋白將為進一步闡述胰腺癌的發(fā)生發(fā)展機制提供很好的線索和幫助。
[Abstract]:Objective to determine the difference of protein expression profiles between pancreatic cancer tissues and normal pancreatic tissues by differential proteomics, and to elucidate the relationship between the changes of protein expression levels and the occurrence and development of pancreatic cancer. Methods six pairs of pancreatic cancer tissues and corresponding normal pancreatic tissues were selected and total proteins were extracted by two-dimensional polyacrylamide gel electrophoresis. The differentially expressed protein spots were obtained by PDQuest software analysis. The differential protein spots were digested and hydrolyzed by electrospray quadrupole mass spectrometry (ESI-Q-TOF MS/MS). The MASCOT search engine was used to search the ExPASy protein sequence database and the differentially expressed proteins were obtained. Some of the identified proteins were verified by immunohistochemical method. Results A total of 97 sites were identified as differentially expressed by two-dimensional gel electrophoresis, 31 of which were successfully identified, 23 proteins were up-regulated and 8 proteins were down-regulated. These proteins are involved in different functional processes and protein families in vivo, including glycolytic enzymes, hyaluronidase, phase 鈪，
本文編號：2312383
[Abstract]:Objective to determine the difference of protein expression profiles between pancreatic cancer tissues and normal pancreatic tissues by differential proteomics, and to elucidate the relationship between the changes of protein expression levels and the occurrence and development of pancreatic cancer. Methods six pairs of pancreatic cancer tissues and corresponding normal pancreatic tissues were selected and total proteins were extracted by two-dimensional polyacrylamide gel electrophoresis. The differentially expressed protein spots were obtained by PDQuest software analysis. The differential protein spots were digested and hydrolyzed by electrospray quadrupole mass spectrometry (ESI-Q-TOF MS/MS). The MASCOT search engine was used to search the ExPASy protein sequence database and the differentially expressed proteins were obtained. Some of the identified proteins were verified by immunohistochemical method. Results A total of 97 sites were identified as differentially expressed by two-dimensional gel electrophoresis, 31 of which were successfully identified, 23 proteins were up-regulated and 8 proteins were down-regulated. These proteins are involved in different functional processes and protein families in vivo, including glycolytic enzymes, hyaluronidase, phase 鈪，
本文編號：2312383
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