本文關(guān)鍵詞： 油氣微生物勘探 高通量測(cè)序 群落結(jié)構(gòu) 微生物多樣性　出處：《應(yīng)用與環(huán)境生物學(xué)報(bào)》2017年02期 　論文類型：期刊論文

【摘要】：精確識(shí)別活性微生物標(biāo)識(shí)物是目前微生物油氣勘探的技術(shù)核心,為深入認(rèn)識(shí)各典型油氣藏上方油氣微生物標(biāo)識(shí)物,采用高通量測(cè)序技術(shù)研究玉北地區(qū)油氣富集帶上方的油氣微生物物種豐度、分布、群落結(jié)構(gòu)等特征.結(jié)果顯示油氣點(diǎn)上方樣品較之背景非油氣點(diǎn)具有更多的特有物種,其中油氣點(diǎn)樣品中含199種,背景非油氣點(diǎn)樣品中含77種.從中共鑒定出10種主要油氣微生物,發(fā)現(xiàn)其中的3類優(yōu)勢(shì)物種(假單胞菌屬、芽孢桿菌屬和土壤桿菌屬)是利用烴類兼性生長的微生物;同時(shí)通過解析統(tǒng)計(jì)學(xué)差異值較高的生物標(biāo)記物時(shí),發(fā)現(xiàn)兩種統(tǒng)計(jì)學(xué)差異值大于5的微生物,分別為背景非油氣點(diǎn)上的弧菌科Vibrionaceae維氏氣單胞菌和油氣點(diǎn)上的方腸桿菌科Erythrobacteraceae赤桿菌.本研究可為油氣微生物勘探提供直接實(shí)驗(yàn)依據(jù).
[Abstract]:Accurate identification of active microbial markers is the core of microbial oil and gas exploration technology, in order to understand the typical oil and gas reservoirs above the oil and gas microbial markers. High throughput sequencing technique was used to study the abundance and distribution of microbial species above the oil-gas enrichment zone in Yubei area. The results show that the samples above the oil and gas point have more endemic species than the background non-oil and gas spots, among which 199 species are found in the oil and gas point samples. Background there are 77 species in non-oil and gas samples. Ten main oil and gas microbes were identified and 3 dominant species (Pseudomonas) were found. Bacillus and Agrobacterium) are microbes that use hydrocarbon facultative growth; At the same time, by analyzing the biomarkers with high statistical difference, we found two kinds of microbes with statistical difference value greater than 5. They are Vibrionaceae Vickers on the background and Erythrobacteraceae gibberellae on the oil and gas sites respectively. The results of this study are as follows:. Oil and gas microbial exploration provides direct experimental basis.
【作者單位】： 浙江大學(xué)地球科學(xué)系;中國石油化工股份有限公司石油勘探開發(fā)研究院無錫石油地質(zhì)研究所;
【基金】：中國石化股份公司科技部項(xiàng)目(P14042) 國家自然科學(xué)基金項(xiàng)目(41202241,41372131,41172112)資助~~
【分類號(hào)】：P618.13;P632.4
【正文快照】： 油氣微生物勘探技術(shù)(Microbial Prospecting for Oil andGas,MPOG)是研究近地表土壤層中微生物異常與地下深部油氣藏關(guān)系的地表勘探法的一個(gè)分支[1].油氣微生物勘探從屬于地質(zhì)微生物學(xué).它是以地質(zhì)學(xué)和生物學(xué),特別是地球化學(xué)和微生物學(xué)為基礎(chǔ)而發(fā)展起來的交叉邊緣學(xué)科.經(jīng)過60多，

本文編號(hào)：1469193