本文選題：自養(yǎng)微生物同化碳 + 活性有機(jī)碳庫��； 參考：《湖南農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：自養(yǎng)微生物在土壤中廣泛存在,且具備光合固碳(同化大氣CO2)功能。然而,在不同土壤利用方式下,自養(yǎng)微生物同化碳在土壤有機(jī)碳各組分及物理和化學(xué)組分中的分配目前尚不明確。為此,本研究采用碳同位素(14C-C02)連續(xù)標(biāo)記技術(shù)結(jié)合室內(nèi)模擬培養(yǎng)試驗(yàn),采用土壤有機(jī)質(zhì)、物理和化學(xué)分組方法,研究了3種不同稻田和旱地土壤自養(yǎng)微生物的固碳量及其在供試土壤物理和化學(xué)組分中的含量與分配特征。結(jié)果表明：1.在稻田與旱地土壤中,自養(yǎng)微生物同化碳的含量為6.21～67.85 mg·kg-l,對土壤有機(jī)碳的貢獻(xiàn)(14C-SOC/SOC)達(dá)6.21-67.85%。其中,在土壤的活性有機(jī)碳庫中,自養(yǎng)微生物同化碳在土壤微生物量碳(14C-MBC)中的含量為5.10～17.87 mg·kg-1,大于自養(yǎng)微生物同化碳在土壤可溶性有機(jī)碳(14C-DOC)中的含量(0.71-4.02 mg-kg-1).土壤自養(yǎng)微生物同化碳對土壤微生物量碳的貢獻(xiàn)(14C-MBC/MBC)為1.14～10.07%,對可溶性有機(jī)碳的貢獻(xiàn)(14C-DOC/DOC)為0.93～8.94%,即MBC的貢獻(xiàn)大于DOC的貢獻(xiàn)。與此同時(shí),在稻田土壤中自養(yǎng)微生物同化碳(14C-SOC)、土壤微生物量碳(14C-MBC)的含量大于旱地,而土壤可溶性有機(jī)碳(14C-DOC)在旱地土壤中的含量大于稻田土壤。2.土壤自養(yǎng)微生物同化碳,主要分布在土壤0.02-0.2mm和0.2-2.0mm大團(tuán)聚體的重組組分大顆粒中,在土壤0.002-0.02mm和0.002mm小團(tuán)聚體重組分中的含量較少,表現(xiàn)出明顯的碳匯效應(yīng)。同時(shí),自養(yǎng)微生物同化碳對土壤大團(tuán)聚體及其土壤重組組分的貢獻(xiàn),要分別大于土壤小團(tuán)聚體及其重組組分；同時(shí),無論是土壤大團(tuán)聚體或小團(tuán)聚體,自養(yǎng)微生物同化碳對其重組組分的貢獻(xiàn)大于輕組組分。此外,各團(tuán)聚體自養(yǎng)微生物同化碳在稻田土壤中的含量大于旱地土壤。3.在稻田與旱地土壤中,土壤自養(yǎng)微生物同化碳,均不同程度地進(jìn)入土壤腐殖質(zhì)的胡敏素(HM)、胡敏酸(HA)和富啡酸(FA)組分；胡敏素、胡敏酸和富啡酸的白養(yǎng)微生物同化碳含量分別為2.21～49.52 mg·kg-1、2.71～12.75 mg·kg-1和1.74～17.37 mg·kg-1。同時(shí),胡敏素組分中14C-SOC對土壤總14C-SOC含量和胡敏素組分SOC含量的貢獻(xiàn)也較其他組分大,分別為33.05～77.48%和0.07-0.57%。此外,稻田土壤中的腐殖質(zhì)各組分自養(yǎng)微生物同化碳含量,大于旱地土壤中的腐殖質(zhì)各組分自養(yǎng)微生物同化碳含量。
[Abstract]:Autotrophic microorganisms exist widely in soil and have the function of carbon sequestration (assimilation of atmospheric CO _ 2). However, under different soil use patterns, the distribution of assimilated carbon from autotrophic microorganisms in soil organic carbon components, physical and chemical components is still unclear. Therefore, in this study, carbon isotope (14C-C02) continuous labeling technique was used in combination with laboratory simulated culture experiments, and soil organic matter, physical and chemical grouping methods were used. Carbon sequestration of three autotrophic microorganisms in paddy and dry land soils and their physical and chemical components and their distribution characteristics were studied. The result shows that 1: 1. The assimilation carbon content of autotrophic microorganisms was 6.2167.85 mg 路kg ~ (-1), and the contribution to soil organic carbon (14C-SOC / SOC) was 6.21-67.85 mg 路kg ~ (-1) in paddy field and dry land soil. In the soil active organic carbon pool, the content of autotrophic microbial assimilation carbon in soil microbial biomass carbon (14C-MBC) was 5.10 ~ 17.87 mg / kg ~ (-1), which was higher than that in soil soluble organic carbon (14C-DOC) (0.71-4.02 mg-kg-1). The contribution of soil autotrophic microbial assimilation carbon to soil microbial biomass carbon (14C-MBC / MBC) was 1.1410.07, and that to soluble organic carbon (14C-DOC / DOC) was 0.93C / DOC 8.94.The contribution of MBC was greater than that of DOC. At the same time, the contents of soil microbial biomass carbon (14C-MBC) and soil soluble organic carbon (14C-DOC) in paddy soils were higher than those in dryland soils. Soil autotrophic microbes assimilate carbon mainly in the recombined fractions of soil 0.02-0.2mm and 0.2-2.0mm aggregates and in the small aggregates of 0.002-0.02mm and 0.002mm with less carbon sequestration effect. At the same time, the contribution of autotrophic microbial assimilation carbon to soil macroaggregates and soil recombination components was greater than that of soil small aggregates and their recombined components, respectively, and the contribution of autotrophic microorganism assimilation carbon to soil macroaggregates and soil recombination components was greater than that of soil aggregates and their recombined components. The contribution of carbon assimilation by autotrophic microorganisms to its recombined components was greater than that of light components. In addition, the assimilation carbon content of autotrophic microorganisms in paddy soil was higher than that in dryland soil. Soil autotrophic microbes assimilate carbon into soil humus (HM), Hu Min acid (HA) and enkephalic acid (FA) in paddy and dryland soils. The assimilation carbon contents of Hu Min acid and fulvic acid were 2.2149.52 mg / kg ~ (-1) kg-1 and 1.74 ~ 17.37 mg / kg ~ (-1), respectively. At the same time, the contribution of 14C-SOC to soil total 14C-SOC and humin component SOC content was also greater than that of other components (33.05% 77.48% and 0.07-0.57%, respectively). In addition, the assimilation carbon content of autotrophic microorganisms in paddy soil was higher than that in dryland soil.
【學(xué)位授予單位】：湖南農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S154.3

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