本文選題：根種病 + 蕓薹根腫菌　； 參考：《揚(yáng)州大學(xué)學(xué)報(bào)(農(nóng)業(yè)與生命科學(xué)版)》2016年04期

【摘要】：采集湖北利川結(jié)球甘藍(lán)病發(fā)生嚴(yán)重區(qū)域的病土和病根,通過(guò)形態(tài)和PCR技術(shù)鑒定,發(fā)現(xiàn)病原菌的形態(tài)特征與蕓薹根腫菌(Plasmodiophora brassicae Wor.)相似,蕓薹根腫菌檢測(cè)引物從被感染根組織中擴(kuò)增到特異帶,明確為蕓薹根腫菌。隨機(jī)選2條特異帶進(jìn)行序列分析,均與P.brassicae ITS保守區(qū)域序列高度同源,一致性達(dá)到99%以上,而且Plasmo3-4擴(kuò)增特異帶序列囊括在PbITS1-2擴(kuò)增的特異帶中。隨后,Plasmo3-4引物在10個(gè)區(qū)域根腫菌和19個(gè)十字花科代表作物進(jìn)行了檢測(cè),所有根腫菌擴(kuò)增出近300bp特異帶,所有作物中沒(méi)有任何條帶,表明Plasmo3-4特異引物可以直接快速地用于根腫菌檢測(cè);利川病原菌檢測(cè)后,進(jìn)一步利用歐洲ECD鑒別系統(tǒng)鑒定此蕓薹根腫菌生理小種為ECD16/4/0,致病力一般。蕓薹根腫菌及其致病性鑒定為抗病研究和抗病育種奠定了基礎(chǔ)。
[Abstract]:The disease soil and disease root were collected in the serious area of Hubei Lichuan condense cabbage disease in Hubei. The morphological characteristics and the Plasmodiophora brassicae Wor. were found to be similar. The primers of Brassica root detected from the infected root tissue to the specific band, which were specifically Brassica. 2 special items were selected. The sequence analysis of the isozonal was highly homologous to the P.brassicae ITS conservative region sequence, the consistency reached more than 99%, and the specific band sequence of the Plasmo3-4 amplification was included in the specific bands of the PbITS1-2 amplification. Subsequently, the Plasmo3-4 primers were detected in 10 regions of root swell and 19 cruciferous plants, all of which were amplified by nearly 30. 0bp specific band, no strip in all crops, indicates that Plasmo3-4 specific primers can be directly used for the detection of root swollen bacteria. After detection of Lichuan pathogenic bacteria, the European ECD identification system has been further used to identify the physiological races of the Brassica root to be ECD16/4/0, and the pathogenicity of Brassica and its pathogenicity identification is the disease resistance research and The foundation of disease resistance breeding is laid.

【作者單位】： 揚(yáng)州大學(xué)園藝與植物保護(hù)學(xué)院;江蘇省農(nóng)業(yè)科學(xué)院蔬菜研究所;利川市汪營(yíng)鎮(zhèn)農(nóng)業(yè)服務(wù)中心;
【基金】：國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系項(xiàng)目(CARS-25) 江蘇省科技支撐計(jì)劃項(xiàng)目(BE2013429) 江蘇省農(nóng)業(yè)科技自主創(chuàng)新基金項(xiàng)目[CX(13)2004]
【分類(lèi)號(hào)】：S432.4

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