本文關(guān)鍵詞： 豆禾間作 nif H基因 熒光定量PCR 高通量測(cè)序 群落組成　出處：《應(yīng)用生態(tài)學(xué)報(bào)》2017年03期 　論文類型：期刊論文

【摘要】：利用熒光定量PCR和高通量測(cè)序的研究方法,比較了3種種植模式(燕麥單作,O;大豆/燕麥,OSO;綠豆/燕麥,OMO)對(duì)燕麥土壤固氮微生物數(shù)量和群落組成的影響.結(jié)果表明:與大豆和綠豆間作顯著改變了燕麥土壤的理化性質(zhì).燕麥土壤固氮微生物nif H基因拷貝數(shù)為每克干土1.75×10~(10)~7.37×10~(10),拔節(jié)期和成熟期OSO和OMO中nif H基因拷貝數(shù)分別是O中的2.18、2.64和1.92、2.57倍,且各處理成熟期nif H基因拷貝數(shù)顯著低于拔節(jié)期.樣品稀釋性曲線和覆蓋度結(jié)果表明,各樣品nif H基因測(cè)序結(jié)果可靠.與綠豆間作顯著提高了燕麥土壤nif H基因的多樣性.各樣品固氮微生物屬水平上優(yōu)勢(shì)類群均為Azohydromonas、固氮菌屬、慢生根瘤菌屬、Skermanella和在屬水平上無(wú)法歸類的固氮微生物,但各優(yōu)勢(shì)類群相對(duì)豐度存在差異.樣品OTU的venn分布和主成分分析顯示,拔節(jié)期和成熟期nif H基因群落結(jié)構(gòu)存在差異,兩個(gè)生育時(shí)期OSO和OMO具有更相似的nif H基因群落結(jié)構(gòu).表明與大豆和綠豆間作可顯著提高燕麥土壤固氮微生物的數(shù)量,并影響固氮微生物的群落組成.
[Abstract]:Fluorescence quantitative PCR and high-throughput sequencing were used to compare three cropping patterns (Oat monoculture). Soybean / oatmeal Oso; Mung beans / oats. OMOO). The results showed that intercropping with soybean and mung bean significantly changed the physical and chemical properties of oat soil. Nitrogen fixation microorganism nif in oat soil. The copy number of H gene is 1.75 脳 10 ~ (1) / g dry soil. 10 (7. 37 脳 10 ~ (10)). The copy numbers of nif H gene in OSO and OMO at jointing stage and mature stage were 2.18 ~ 2.64 and 1.92 ~ 2.57 times of those in O, respectively. The copy number of nif H gene was significantly lower than that at jointing stage. The dilution curve and coverage of the sample showed that the number of copies of nif H gene was significantly lower than that at jointing stage. The results of nif H gene sequencing in each sample were reliable. The nif in oat soil was significantly improved by intercropping with mung bean. The diversity of H gene. The dominant group of nitrogen-fixing microorganism in all samples was Azohydromonas. Azotobacter, Rhizobium, Skermanella and nitrogen-fixing microbes that cannot be classified at the generic level. The venn distribution and principal component analysis of OTU showed that there were differences in the community structure of nif H gene between jointing stage and mature stage. OSO and OMO had more similar nif H gene community structure in the two growing periods, indicating that intercropping with soybean and mung bean could significantly increase the amount of nitrogen-fixing microorganisms in oat soil. The community composition of nitrogen-fixing microorganisms was also affected.
【作者單位】： 中國(guó)農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;中國(guó)農(nóng)業(yè)科學(xué)院作物科學(xué)研究所/農(nóng)業(yè)部作物生理生態(tài)重點(diǎn)實(shí)驗(yàn)室;吉林省白城市農(nóng)業(yè)科學(xué)院;
【基金】：國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)(CARS-08-B-1) 公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201503121-11,201503120)資助~~
【分類號(hào)】：S154.3;S344.2
【正文快照】： 3吉林省白城市農(nóng)業(yè)科學(xué)院,吉林白城137000)本研究由國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)(CARS-08-B-1)和公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201503121-11,201503120)資助This study wassupported by the China Agriculture Research System Project(CARS-08-B-1)and the Special Fund fo

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