【摘要】：采用同源克隆技術(shù)分離了3種錦雞兒屬植物檸條錦雞兒(Caragana korshinskii)、小葉錦雞兒(C.microphylla)和中間錦雞兒(C.intermedia)的過氧化物酶(POD)基因(分別命名為CkPOD、CmPOD和CiPOD),并對它們在干旱脅迫條件下的表達特征進行了分析。CkPOD、CiPOD、CmPOD基因的cDNA序列均包含有1 074bp的開放閱讀框(ORF),編碼的蛋白質(zhì)由357個氨基酸構(gòu)成,分子量為38.7kD。系統(tǒng)進化分析結(jié)果顯示:3種錦雞兒屬植物的POD可以和鷹嘴豆等豆科植物的POD聚為一類,且CkPOD和CmPOD具有較近的親緣關(guān)系,CiPOD與CkPOD和CmPOD的親緣關(guān)系相對較遠,這一結(jié)果與3種錦雞兒屬植物的進化地位一致,顯示POD基因較為保守,可以為錦雞兒屬植物的系統(tǒng)分類提供參考。PEG模擬干旱脅迫能夠強烈誘導(dǎo)CkPOD、CiPOD和CmPOD基因的表達,顯示POD基因在錦雞兒屬植物抵御干旱脅迫過程中發(fā)揮著重要作用。研究結(jié)果可為解析錦雞兒屬植物的耐旱機理以及利用錦雞兒屬植物進行荒漠改良和生態(tài)修復(fù)提供理論和實驗依據(jù)。
[Abstract]:The peroxidase (POD) genes (named CkPOD,CmPOD and CiPOD),) of Caragana (Caragana korshinskii), (C.microphylla) and Caragana intermedia (C.intermedia) were isolated by homologous cloning technique. Their expression characteristics under drought stress were analyzed. The cDNA sequences of the CkPOD,CiPOD,CmPOD gene were composed of 357 amino acids and the molecular weight was 38.7 KD. All the cDNA sequences of the CkPOD,CiPOD,CmPOD gene contained an open reading frame (ORF),) with 1 074bp. Phylogenetic analysis showed that the POD of three species of Caragana could be clustered into a group of POD of legumes such as chickpea, and CkPOD and CmPOD had close relationship, and CiPOD had a relatively distant relationship with CkPOD and CmPOD. This result is consistent with the evolutionary status of three species of Caragana, indicating that the POD gene is conserved and can provide a reference for the systematic classification of Caragana plants. PEG mimic drought stress can strongly induce the expression of CkPOD,CiPOD and CmPOD genes. POD gene plays an important role in the resistance of Caragana to drought stress. The results can provide theoretical and experimental basis for the analysis of drought tolerance mechanism of Caragana plants and for desert improvement and ecological restoration of Caragana plants.
【作者單位】： 內(nèi)蒙古大學(xué)生命科學(xué)學(xué)院;內(nèi)蒙古農(nóng)業(yè)大學(xué)林學(xué)院;
【基金】：國家自然科學(xué)基金(31260168) 內(nèi)蒙古自然科學(xué)基金(2015MS0355)
【分類號】：Q943.2

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