【摘要】：目的 檢測(cè)SOX15基因于結(jié)腸癌中的表達(dá)高低以及對(duì)細(xì)胞增殖、凋亡、遷移、侵襲的影響。方法 RT-PCR被用于檢測(cè)SOX15在Caco2、SW480、HT29、HCT116結(jié)腸癌細(xì)胞以及正常結(jié)腸組織中的表達(dá)程度高低。通過(guò)脂質(zhì)體Lipofectamine 2000將重組質(zhì)粒pEGFP-N1-SOX15與空質(zhì)粒pEGFP-N1轉(zhuǎn)染入Caco2及SW480結(jié)腸癌細(xì)胞后,應(yīng)用RT-PCR及Western blot分別檢測(cè)SOX15在Caco2及SW480細(xì)胞中mRNA以及蛋白質(zhì)表達(dá)水平;CCK-8檢測(cè)SOX15對(duì)Caco2及SW480細(xì)胞增殖的影響、克隆形成觀察癌細(xì)胞克隆形成率、流式細(xì)胞儀分析細(xì)胞凋亡率、Transwell檢測(cè)SOX15對(duì)細(xì)胞遷移、侵襲功能的調(diào)節(jié)控制。結(jié)果 與人正常結(jié)腸組織相比,SOX15在Caco2、SW480、HT29、HCT116結(jié)腸癌細(xì)胞中mRNA及蛋白質(zhì)表達(dá)水平明顯低于其在正常結(jié)腸組織的表達(dá)水平。p EGFP-N1-SOX15轉(zhuǎn)染組細(xì)胞mRNA和蛋白表達(dá)顯著高于p EGFP-N1對(duì)照組細(xì)胞。與pEGFP-N1對(duì)照組相比,pEGFP-N1-SOX15轉(zhuǎn)染組可明顯抑制細(xì)胞增殖及遷移侵襲能力,抑制癌細(xì)胞克隆形成率,并誘導(dǎo)細(xì)胞凋亡。結(jié)論 SOX15在結(jié)腸癌細(xì)胞中低表達(dá),過(guò)表達(dá)SOX15可明顯抑制結(jié)腸癌細(xì)胞的增殖及遷移、侵襲能力,并促進(jìn)細(xì)胞凋亡。
[Abstract]:Objective to detect the expression of SOX15 gene in colon cancer and its effect on cell proliferation, apoptosis, migration and invasion. Methods RT-PCR was used to detect the expression of SOX15 in Caco2,SW480,HT29,HCT116 colon cancer cells and normal colon tissues. The recombinant plasmid pEGFP-N1-SOX15 and empty plasmid pEGFP-N1 were transfected into Caco2 and SW480 colon cancer cells by liposome Lipofectamine 2000. The mRNA and protein expression levels of SOX15 in Caco2 and SW480 cells were detected by RT-PCR and Western blot respectively. The effect of SOX15 on the proliferation of Caco2 and SW480 cells was detected by CCK-8. Clone formation was observed and apoptosis rate was analyzed by flow cytometry. Transwell was used to detect the regulation of cell migration and invasion by SOX15. Results compared with normal colon tissue, the expression of mRNA and protein in Caco2,SW480,HT29,HCT116 colon cancer cells was significantly lower than that in normal colon tissues. The expression of mRNA and protein in the transfected cells of p EGFP-N1-SOX15 was significantly higher than that in the control cells of p EGFP-N1. Compared with pEGFP-N1 control group, pEGFP-N1-SOX15 transfection group could significantly inhibit cell proliferation, migration and invasion, inhibit cancer cell clone formation, and induce apoptosis. Conclusion the expression of SOX15 in colon cancer cells is low. Overexpression of SOX15 can significantly inhibit the proliferation, migration, invasion and apoptosis of colon cancer cells.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.35

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本文編號(hào)：2209062