本文選題：水貂 + TYR基因��； 參考：《中國畜牧獸醫(yī)》2017年10期

【摘要】：試驗旨在檢測水貂酪氨酸酶(tyrosinase,TYR)基因T138A位點的多態(tài)性,并分析其與水貂毛色表型的相關(guān)性。提取5種被毛色型430只水貂血液基因組DNA,采用PCR-RFLP技術(shù),對TYR基因T138A位點進行多態(tài)性檢測,統(tǒng)計等位基因頻率與基因型頻率,通過卡方(χ2)獨立性檢驗分析該位點多態(tài)性與水貂毛色性狀的相關(guān)性。結(jié)果表明,T138A位點存在2個等位基因T和A,形成TT、TA和AA 3種基因型,AA基因型在吉林白水貂群體中為優(yōu)勢基因型(0.9069),而TT基因型為金州黑水貂、珍珠水貂、咖啡水貂和銀藍水貂群體的優(yōu)勢基因型,其中在金州黑水貂群體中基因型頻率最高(1.0000)。關(guān)聯(lián)分析表明,TYR基因T138A位點的多態(tài)性與毛色性狀呈極顯著相關(guān)(P0.0001)。表明TYR基因T138A位點可能是影響水貂毛色的主控位點或與調(diào)控白色被毛表型主控位點連鎖的分子標記。
[Abstract]:The aim of this study was to detect the polymorphism of the T138A locus of the mink tyrosinase (TYR) gene and to analyze its association with the fur phenotype of mink. The genomic DNA was extracted from 430 minks of five kinds of hairy patterns. The polymorphism of T138A locus of TYR gene was detected by PCR-RFLP. The frequency of alleles and genotypes were analyzed. Chi-square (蠂 2) independence test was used to analyze the correlation between the polymorphism of this locus and the fur color traits of mink. The results showed that there were two alleles T and A at T138A locus. The three genotypes, TTTA and AA, were the dominant genotype (0.9069) in Jilin white mink population, while the TT genotype was Jinzhou black mink and pearl mink. The dominant genotypes of coffee mink and silver blue mink were the highest in Jinzhou black mink population (1.0000). The association analysis showed that the polymorphism of T138A locus of TYR gene was significantly correlated with the coat color traits (P0.0001). It was suggested that T138A locus of the TYR gene might be the main control site affecting the coat color of mink or a molecular marker linked to the dominant locus regulating the white coat phenotype.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院特產(chǎn)研究所 吉林省特種經(jīng)濟動物分子生物學(xué)省部共建國家重點實驗室;
【基金】：國家重點基礎(chǔ)研究發(fā)展計劃-973項目(2012CB722907) 水貂優(yōu)良種質(zhì)資源及選育技術(shù)引進-948項目(2014-Z8) 中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程—特種動物育種科技創(chuàng)新團隊(CAAS-ASTIP-2016-ISAPS02) 中央級公益性科研院所基本科研業(yè)務(wù)費專項(1610342017011)
【分類號】：S865.22
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本文編號：2066142