【摘要】：采用密閉微波輔助法(PMAE)提取還亮草中的硬飛燕草堿和巴比翠雀堿。采用單因素試驗(yàn)結(jié)合正交試驗(yàn)方法對(duì)微波實(shí)驗(yàn)條件進(jìn)行優(yōu)化。得到硬飛燕草堿和巴比翠雀堿的最佳提取方案:藥物顆粒度100目,固液比1∶60,微波溫度80℃,微波功率560 W,微波時(shí)間10 min。以甲醇-0.2%三乙胺(45∶55)為流動(dòng)相,建立了高效液相色譜(HPLC)測(cè)定硬飛燕草堿和巴比翠雀堿含量的方法。硬飛燕草堿和巴比翠雀堿分別在0.50~50.0,0.30~30.0 mg/L范圍內(nèi)呈良好的線性關(guān)系,回收率為98.3%~104.5%,相對(duì)標(biāo)準(zhǔn)偏差(RSD)分別為2.0%和2.2%。與傳統(tǒng)溶劑回流法(SRE)進(jìn)行比較,該方法簡(jiǎn)單、提取率更高。
[Abstract]:A closed microwave assisted (PMAE) method was used to extract the alkaloids and barbiturine from Hedyotis huanliensis. The condition of microwave experiment was optimized by single factor test and orthogonal test. The optimum extraction scheme of Euphorbiferine and barbitidine was obtained as follows: drug particle size 100 mesh, solid-liquid ratio 1: 60, microwave temperature 80 鈩，

本文編號(hào)：2227544