發(fā)布時(shí)間：2018-06-15 16:55

  本文選題：槲皮素 + 蘆丁�。� 參考：《合肥工業(yè)大學(xué)》2017年碩士論文

【摘要】：槲皮素和蘆丁是自然界中存在的最豐富的天然黃酮類(lèi)抗氧化劑,其中蘆丁是槲皮素的蕓香糖苷,兩者通常共存于許多植物中。文獻(xiàn)中已有對(duì)兩者進(jìn)行同時(shí)電化學(xué)檢測(cè)的報(bào)道,但由于兩者氧化峰的重疊而遇到困難。本文內(nèi)容包括兩部分,分別通過(guò)改進(jìn)常規(guī)電化學(xué)方法和采用全新的雙極電化學(xué)方法對(duì)槲皮素和蘆丁進(jìn)行電化學(xué)分離檢測(cè)。將石墨片電極進(jìn)行預(yù)氧化活化處理,對(duì)槲皮素和蘆丁的電化學(xué)反應(yīng)過(guò)程進(jìn)行伏安研究,了解其反應(yīng)機(jī)理,并為優(yōu)化電化學(xué)分離檢測(cè)提供參考。另一方面,通過(guò)改變支持電解質(zhì)濃度,以增強(qiáng)槲皮素和蘆丁在電極上的吸附及電氧化信號(hào)。對(duì)天然抗氧化劑的電化學(xué)研究,本身也有助于理解其抗氧化活性及相關(guān)的藥理作用機(jī)制。以電化學(xué)預(yù)處理的石墨片為工作電極,對(duì)槲皮素的多步氧化反應(yīng)進(jìn)行了伏安掃描測(cè)試,得到了 4個(gè)陽(yáng)極峰和6個(gè)反向陰極峰,電流峰的數(shù)目多于文獻(xiàn)報(bào)道;考察了電勢(shì)掃描范圍、電解液pH值、富集時(shí)間、掃描速率、槲皮素濃度等對(duì)各伏安電流峰的影響;提出了槲皮素第1和第3陽(yáng)極峰的連串氧化反應(yīng)機(jī)理,使對(duì)槲皮素這一重要的天然黃酮化合物的氧化代謝機(jī)理的認(rèn)識(shí)深化了一步。結(jié)果表明經(jīng)氧化預(yù)處理的石墨片電極的性能優(yōu)于未經(jīng)過(guò)氧化預(yù)處理的石墨片電極。再用石墨片電極對(duì)蘆丁的多步氧化反應(yīng)進(jìn)行實(shí)驗(yàn),實(shí)驗(yàn)結(jié)果得到了蘆丁的兩個(gè)增強(qiáng)的氧化峰。增大支持電解質(zhì)濃度,也達(dá)到了增強(qiáng)槲皮素和蘆丁的電氧化信號(hào)的目的。但兩者的電氧化峰的重疊問(wèn)題未得到改善�；谏鲜�,嘗試運(yùn)用雙極電化學(xué)原理設(shè)計(jì)全新的薄層微流控雙極電化學(xué)電解池,旨在利用微通道中溶液中存在的梯度電勢(shì)差,對(duì)槲皮素和蘆丁進(jìn)行分離檢測(cè)。根據(jù)測(cè)試結(jié)果,對(duì)雙極電化學(xué)微通道流動(dòng)反應(yīng)池的結(jié)構(gòu)進(jìn)行了持續(xù)改進(jìn),逐步解決了雙極池運(yùn)行過(guò)程中出現(xiàn)的一些困難和技術(shù)問(wèn)題。將該方法應(yīng)用于多組分電化學(xué)分離檢測(cè)的思路具有很強(qiáng)的探索性,目前尚未能達(dá)到最終的目標(biāo),但為后續(xù)的改進(jìn)提供了經(jīng)驗(yàn)和信心。
[Abstract]:Quercetin and rutin are the most abundant natural flavonoids antioxidants in nature, among which rutin is the rutin glycoside of quercetin, which usually coexist in many plants. The simultaneous electrochemical detection of both has been reported in the literature, but it is difficult because of the overlap of oxidation peaks. This paper consists of two parts. The electrochemical separation of quercetin and rutin is carried out by improving the conventional electrochemical method and using a new bipolar electrochemical method. The electrochemical reaction of quercetin and rutin was studied by voltammetry. The mechanism of the reaction was understood and the reference was provided for the optimization of electrochemical separation and detection. On the other hand, the adsorption and electrooxidation signals of quercetin and rutin on the electrode were enhanced by changing the concentration of supporting electrolyte. The electrochemical study of natural antioxidants also helps to understand their antioxidant activity and related pharmacological mechanisms. The multistep oxidation reaction of quercetin was measured by voltammetry with graphite sheet pretreated by electrochemical method. Four anodic peaks and six reverse cathodic peaks were obtained, and the number of current peaks was more than that reported in literature. The effects of potential scanning range, electrolyte pH value, enrichment time, scanning rate and quercetin concentration on the volt-ampere current peaks were investigated, and the mechanism of successive oxidation of the first and third anodic peaks of quercetin was proposed. The mechanism of oxidation metabolism of quercetin, an important natural flavonoid, is further understood. The results show that the performance of the graphite sheet electrode pretreated by oxidation is better than that of the graphite sheet electrode without preoxidation. The multistep oxidation reaction of rutin was carried out with graphite electrode. Two enhanced oxidation peaks of rutin were obtained. Increasing the concentration of supporting electrolyte also enhances the electrooxidation signal of quercetin and rutin. However, the overlap of the two electrooxidation peaks has not been improved. Based on the above, a novel thin-layer microfluidic bipolar electrolysis cell was designed by using the bipolar electrochemistry principle. The aim was to separate quercetin from rutin by using the gradient potential difference in the solution of the microchannel. According to the test results, the structure of the bipolar electrochemical microchannel flow reaction cell has been continuously improved, and some difficulties and technical problems in the operation of the bipolar cell have been solved step by step. The idea of applying this method to multicomponent electrochemical separation and detection is very exploratory, but it can not reach the final goal yet, but it provides experience and confidence for further improvement.
【學(xué)位授予單位】：合肥工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：O657.1;TQ28

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本文編號(hào)：2022751