【摘要】：考察了濃度為10、50和100 mg·L~(-1)的多壁碳納米管(MWCNTs)的植物毒性,通過測(cè)量水稻生長(zhǎng)指數(shù)、根系氧化應(yīng)激反應(yīng)、細(xì)胞膜損傷和生理功能的變化,探索了其具體的毒性機(jī)制。將水稻幼苗暴露于不同濃度的MWCNTs懸浮液中培養(yǎng)10 d后,高濃度處理組(100 mg·L~(-1))中水稻幼苗地上部分和根系鮮重分別降低至對(duì)照組的87.6%±1.1%和69.2%±7.8%。對(duì)根系深入研究發(fā)現(xiàn)氧化應(yīng)激反應(yīng)和細(xì)胞膜的損傷主要出現(xiàn)在高濃度處理組,此時(shí)過氧化氫酶(CAT)活性由對(duì)照組的(8.8±1.6)U·mg-1prot(protein,蛋白質(zhì))增加至(16.3±2.8)U·mg-1prot,丙二醛(MDA)含量由對(duì)照組的(8.0±0.3)μmol·g-1FW(fresh weight,鮮重)增加至(15.1±1.4)μmol·g-1FW。然而,水稻根系的生化酶活性在低濃度(10 mg·L~(-1))時(shí)就開始明顯降低。通過透射電鏡(TEM)觀察發(fā)現(xiàn),MWCNTs顆粒分布在水稻幼苗根系細(xì)胞內(nèi),從而證實(shí)了MWCNTs能被植物細(xì)胞吸收。
[Abstract]:The phytotoxicity of multiwalled carbon nanotubes (MWCNTs) with concentrations of 1050mg / L and 100mg / L ~ (-1) was investigated. The specific toxicity mechanism of MWCNTs was explored by measuring the growth index, root oxidative stress response, cell membrane damage and physiological function changes. After the rice seedlings were exposed to different concentrations of MWCNTs suspension for 10 days, the aboveground part and root fresh weight of rice seedlings in the high concentration treatment group (100 mg L ~ (-1) decreased to 87.6% 鹵1.1% and 69.2% 鹵7.8% of the control group, respectively. It was found that oxidative stress response and cell membrane damage mainly occurred in the high concentration treatment group. The activity of catalase (cat) increased from (8.8 鹵1.6) U mg-1prot (protein) to (16.3 鹵2.8) U mg-1prot. the content of malondialdehyde (MDA) increased from (8.0 鹵0.3) 渭 mol g -1 FW (fresh weight, fresh weight to (15.1 鹵1.4) 渭 mol g -1 FW. However, the biochemical enzyme activity of rice roots began to decrease at low concentration (10 mg / L ~ (-1). Transmission electron microscopy (TEM) showed that MWCNTs particles were distributed in root cells of rice seedlings, which proved that MWCNTs could be absorbed by plant cells.
【作者單位】： 湖南大學(xué)環(huán)境科學(xué)與工程學(xué)院;湖南大學(xué)環(huán)境生物與控制教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：湖南大學(xué)交叉學(xué)科項(xiàng)目(531107040761) 國(guó)家自然科學(xué)基金(51579095)
【分類號(hào)】：X173

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