發(fā)布時(shí)間：2018-04-10 14:49

  本文選題：表面增強(qiáng)拉曼散射 + 磁性納米顆粒�。� 參考：《南京郵電大學(xué)》2015年碩士論文

【摘要】：隨著現(xiàn)代醫(yī)學(xué)的發(fā)展,大量研究證明腫瘤的產(chǎn)生和發(fā)展過(guò)程中都伴隨著一些特定蛋白質(zhì)的出現(xiàn)或者含量變化,檢測(cè)腫瘤標(biāo)志蛋白及其含量有望對(duì)腫瘤實(shí)現(xiàn)早期診斷。表面增強(qiáng)拉曼散射(SERS)用于蛋白質(zhì)檢測(cè)是蛋白質(zhì)檢測(cè)學(xué)與SERS技術(shù)相結(jié)合的新型檢測(cè)技術(shù),在生物醫(yī)學(xué)檢測(cè)領(lǐng)域表現(xiàn)出巨大的應(yīng)用潛力�；谌髦谓Y(jié)構(gòu)的SERS免疫檢測(cè)是常用的一種基于SERS技術(shù)的蛋白質(zhì)檢測(cè)方法,基本組成部分有免疫檢測(cè)基底、待檢測(cè)蛋白、SERS免疫檢測(cè)探針。近年來(lái),磁性納米材料在生物樣品的分離提純方面表現(xiàn)出了突出的優(yōu)勢(shì),是理想的免疫基底材料。本論文中我們可控制備了Fe3O4@SiO2@Au核殼結(jié)構(gòu)這一兼具磁性和SERS活性的納米材料,并進(jìn)一步構(gòu)建得到免疫檢測(cè)基底,結(jié)合花狀金納米顆粒構(gòu)建的SERS免疫探針,實(shí)現(xiàn)了對(duì)腫瘤標(biāo)志蛋白CEA、NSE的高靈敏聯(lián)合免疫檢測(cè)。主要研究?jī)?nèi)容如下:(1)可控制備復(fù)合SERS增強(qiáng)性能的磁性納米材料首先采用共沉積法制備了Fe3O4磁性納米顆粒,隨后對(duì)納米顆粒進(jìn)行二氧化硅包殼,制備Fe3O4@SiO2核殼型納米結(jié)構(gòu),通過(guò)控制正硅酸乙酯的加入劑量調(diào)控了Fe3O4@SiO2的殼厚和包覆磁性顆粒粒子數(shù),并進(jìn)一步吸附金種子后生長(zhǎng)了一層SERS活性金納米殼,制備得到復(fù)合SERS增強(qiáng)性能的磁性納米材料Fe3O4@SiO2@Au。(2)基于SERS活性磁性納米顆粒開(kāi)展腫瘤標(biāo)志蛋白檢測(cè)選擇磁性和結(jié)構(gòu)俱佳、SERS性能優(yōu)異的Fe3O4@SiO2@Au,經(jīng)表面修飾特定抗體構(gòu)建得到用于捕獲待檢測(cè)蛋白的免疫檢測(cè)基底,同時(shí)制備了具有優(yōu)異SERS增強(qiáng)性能的花狀金納米顆粒并構(gòu)建了SERS免疫檢測(cè)探針。免疫識(shí)別后形成三明治結(jié)構(gòu)復(fù)合物,利用外加磁場(chǎng),實(shí)施檢測(cè)樣品的分離與富集,分別對(duì)human-IgG和腫瘤標(biāo)志蛋白CEA實(shí)現(xiàn)了高靈敏、特異性檢測(cè)。(3)腫瘤標(biāo)志蛋白聯(lián)合檢測(cè)為實(shí)現(xiàn)雙元腫瘤蛋白標(biāo)志物的聯(lián)合檢測(cè),我們基于Fe3O4@SiO2@Au材料,設(shè)計(jì)并構(gòu)建了能夠同時(shí)特異性捕獲腫瘤標(biāo)志物CEA和NSE的磁性免疫基底,并分別構(gòu)建了用于檢測(cè)CEA和NSE的高靈敏SERS探針,開(kāi)展并實(shí)現(xiàn)了對(duì)兩種腫瘤標(biāo)志蛋白的低濃度、特異性聯(lián)合檢測(cè)。
[Abstract]:With the development of modern medicine, a large number of studies have proved that the emergence and development of tumors are accompanied by the emergence or content of some specific proteins, the detection of tumor markers and their content is expected to achieve early diagnosis of tumors.Surface enhanced Raman scattering (SERS) for protein detection is a new detection technique combining protein detection with SERS technology. It has great potential in biomedical detection.Sandwich structure based SERS immunoassay is a commonly used protein detection method based on SERS technology.In recent years, magnetic nanomaterials have shown outstanding advantages in the separation and purification of biological samples and are ideal immune substrate materials.In this thesis, we can control the Fe3O4@SiO2@Au core-shell structure, which is a magnetic and SERS active nano-material, and further construct the immunosensory substrate and construct the SERS immune probe combined with the flower-like gold nanoparticles.A highly sensitive combined immunoassay for tumor marker CEANSE was achieved.The main contents of the study are as follows: (1) the magnetic nanocrystalline materials which can control the properties of composite SERS were prepared by co-deposition method, and then Fe3O4@SiO2 core-shell nanostructures were prepared by encapsulating the nanoparticles with silica.By controlling the dosage of ethyl orthosilicate, the shell thickness and the number of particles coated with magnetic particles of Fe3O4@SiO2 were regulated, and a layer of SERS active gold nanoparticles was grown after further adsorption of gold seeds.To capture the immunoassay substrate of the protein to be tested,At the same time, flower-like gold nanoparticles with excellent SERS enhancement properties were prepared and SERS immunoassay probes were constructed.The sandwich structure complex was formed after immunological recognition. The sample was separated and enriched by external magnetic field. The sandwich structure complex was highly sensitive to human-IgG and tumor marker protein CEA, respectively.A highly sensitive SERS probe was constructed for the detection of CEA and NSE, and the low concentration and specificity detection of the two tumor marker proteins was carried out and realized.
【學(xué)位授予單位】：南京郵電大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：TB383.1;O657.37

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