本文選題：鮑曼不動(dòng)桿菌 + 生物膜�。� 參考：《青島大學(xué)》2015年碩士論文

【摘要】：目的探討鮑曼不動(dòng)桿菌臨床分離菌株生物膜形成能力及生物膜相關(guān)基因的分布情況,分析其與細(xì)菌耐藥表型的關(guān)系。方法采用前瞻性研究,收集成武縣人民醫(yī)院2012年10月至2013年10月分離的70株鮑曼不動(dòng)桿菌,采用96孔聚苯乙烯板構(gòu)建生物膜模型,結(jié)晶紫染色法定性、定量分析生物膜形成能力,比較不同生物膜形成能力的鮑曼不動(dòng)桿菌抗菌藥物的耐藥性。運(yùn)用聚合酶鏈反應(yīng)(PCR)技術(shù)擴(kuò)增及檢測(cè)生物膜相關(guān)蛋白Bap,生物膜合成相關(guān)基因bfs和Ⅰ類整合酶基因int I1的表達(dá)情況。結(jié)果70株鮑曼不動(dòng)桿菌中40株為多重耐藥菌,6株為泛耐藥菌。68株(97.14%)具有生物膜形成能力,其中弱陽(yáng)性14株,陽(yáng)性20株,強(qiáng)陽(yáng)性34株。鮑曼不動(dòng)桿菌對(duì)亞胺培南、阿米卡星、美羅培南、頭孢吡肟的耐藥率分別為30.00%、32.86%、38.57%及41.43%,對(duì)其他常用抗菌藥物的耐藥率均不小于50%。且隨著生物膜形成能力的增強(qiáng),鮑曼不動(dòng)桿菌對(duì)左氧氟沙星(χ2=9.225,P=0.010)、頭孢吡肟(χ2=7.222,P=0.027)、慶大霉素(χ2=6.601,P=0.037)的耐藥率顯著降低。生物膜弱陽(yáng)性、陽(yáng)性和強(qiáng)陽(yáng)性鮑曼不動(dòng)桿菌Bap基因陽(yáng)性率分別為50.00%、65.00%和79.41%(χ2=4.244,P=0.120),bfs陽(yáng)性率分別為35.71%、65.00%和88.24%(χ2=13.602,P=0.001),int I1陽(yáng)性率分別為42.86%、75.00%和91.18%(χ2=12.902,P=0.002)。生物膜相關(guān)基因陽(yáng)性組對(duì)抗菌藥物耐藥率大多高于基因陰性組,其中僅int I1陽(yáng)性組對(duì)阿米卡星的耐藥顯著高于陰性組(χ2=5.194,P=0.023)。結(jié)論鮑曼不動(dòng)桿菌多重耐藥情況嚴(yán)峻,且生物膜形成能力較強(qiáng),隨著生物膜形成能力的增強(qiáng)其耐藥性降低;Bap、bfs、int I1基因參與生物膜形成。
[Abstract]:Objective to investigate the biofilm forming ability of Acinetobacter baumannii clinical isolates and the distribution of biofilm related genes, and to analyze the relationship between biofilm related genes and bacterial drug resistance phenotype. Methods A prospective study was conducted to collect 70 strains of Acinetobacter baumannii isolated from October 2012 to October 2013 in Chengwu County people's Hospital. The biofilm model was constructed with 96 hole polystyrene plates. Quantitative analysis of biofilm forming ability and comparison of antimicrobial resistance of Acinetobacter baumannii with different biofilm formation ability. Polymerase chain reaction (PCR) technique was used to amplify and detect the expression of biofilm-associated protein Bap, bfs and int I1. Results among the 70 Acinetobacter baumannii strains, 40 were multidrug resistant and 6 were pan-resistant. 68 strains were pan-resistant. Among them, 14 were weakly positive, 20 were positive and 34 were strongly positive. The drug resistance rates of Acinetobacter baumannii to imipenem, amikacin, meropenem and cefepime were 30.0032. 86% and 41. 43%, respectively. The resistance rates of Acinetobacter baumannii to imipenem, amikacin, meropenem and cefepime were not less than 50%. With the enhancement of biofilm forming ability, the resistance of Acinetobacter baumannii to levofloxacin (蠂 ~ 2 + 9.225), cefepime (蠂 ~ (2) 7.222) and gentamicin (蠂 ~ (2) 6.601) decreased significantly. The positive rates of Bap gene of Acinetobacter baumannii were 65.00% and 79.41% respectively (蠂 2 / 4.244P = 0.120) and the positive rates of BFS were 35.71g% and 88.2400% respectively (蠂 213.602P 0.001T I1 positive rates were 42.86% and 91.1800%, respectively). The antimicrobial resistance rate of biofilm-associated gene positive group was higher than that of gene negative group. Only int I1 positive group was significantly more resistant to amikacin than that of negative group (蠂 ~ 2 ~ 2 ~ (5.194). Conclusion the multidrug resistance of Acinetobacter baumannii is severe and the ability of biofilm formation is strong. The drug resistance of Acinetobacter baumannii is decreased with the increase of biofilm forming ability.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R446.5

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相關(guān)期刊論文 前1條

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本文編號(hào)：1814814