本文選題：金黃色葡萄球菌　切入點(diǎn)：萬(wàn)古霉素耐藥　出處：《中國(guó)感染與化療雜志》2016年04期 　論文類型：期刊論文

【摘要】：目的探討耐甲氧西林金黃色葡萄球菌(金葡菌)(MRSA)對(duì)萬(wàn)古霉素耐藥的折點(diǎn)調(diào)整后,對(duì)異質(zhì)性萬(wàn)古霉素中介金葡菌(h VISA)的平皿篩選方法選擇的影響,尋求一種敏感、有效的平皿篩選方法。方法收集臨床MRSA菌株215株,分別用濃度為6 mg/L、4 mg/L、2 mg/L萬(wàn)古霉素腦心浸液瓊脂平皿(BHIV6、BHIV4、BHIV2)及濃度為5 mg/L替考拉寧腦心浸液瓊脂(BHIT5)進(jìn)行h VISA篩選,同時(shí)應(yīng)用菌群分析/曲線下面積(PAP/AUC)法檢測(cè)h VISA,以PAP/AUC法作為金標(biāo)準(zhǔn),對(duì)比上述4種平皿篩選方法的敏感度及特異度。結(jié)果 PAP/AUC檢測(cè)h VISA的陽(yáng)性菌株分別為12株,BHIV6、BHIV4、BHIV2、BHIT5篩選陽(yáng)性菌株分別為6、18、54、20株;以PAP/AUC法作為金標(biāo)準(zhǔn),上述4種平皿篩選方法靈敏度分別為41.7%、83.3%、91.7%、83.3%,特異度分別為99.0%、95.9%、77.8%、94.8%。結(jié)論 MRSA對(duì)萬(wàn)古霉素耐藥折點(diǎn)調(diào)整后,BHIV6篩選法靈敏度較低、BHIV2篩選法特異度較低,不應(yīng)作為h VISA的篩選方法,BHIV4和BHIT5平皿篩選法靈敏度和特異度均較高,可作為萬(wàn)古霉素耐藥折點(diǎn)調(diào)整后對(duì)h VISA的常規(guī)篩選方法。
[Abstract]:Objective to investigate the effect of MRSA-resistant to vancomycin-resistant Staphylococcus aureus (MRSAs) on the selection of a pandish screening method for heterogeneous vancomycin-mediated staphylococcus aureus (h VISAs), and to find a sensitive method for the screening of vancomycin-resistant Staphylococcus aureus. Methods 215 strains of clinical MRSA strains were collected and screened with 6 mg / L 4 mg / L ~ (2) mg/L vancomycin brain extract (Agar) and 5 mg/L teicolanine brain heart extract (Agar BHIT5) and the concentration of 5 mg/L teicolanine (Agar BHIT5), respectively. At the same time, the method of microflora analysis / area under curve was used to detect hVISA.The PAP/AUC method was used as the gold standard. The sensitivity and specificity of the four screening methods were compared. Results the positive strains of h VISA detected by PAP/AUC were 12 strains of BHIV6, BHIV4, HIV2, HIV2, BHIT5, respectively, and 20 strains were screened by PAP/AUC as gold standard. The sensitivity of the four screening methods were 41.7 and 83.37, and the specificity was 99.00.95. 9 and 77.80.Conclusion the sensitivity of MRSA in screening vancomycin resistant to vancomycin was lower than that of BHIV6, and the sensitivity of HIV6 screening was lower than that of BHIV6, and the specificity was 99.09.90.Conclusion the sensitivity of MRSA for vancomycin resistant screening was lower than that of BHIV6. It should not be used as a screening method for h VISA. Both HIV4 and BHIT5 plate screening methods have higher sensitivity and specificity. They can be used as routine screening methods for h VISA after adjusting vancomycin resistance point.
【作者單位】： 山東省濱州市人民醫(yī)院內(nèi)科;
【基金】：山東省醫(yī)藥衛(wèi)生發(fā)展計(jì)劃(2014WS0310)
【分類號(hào)】：R446.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 劉亞麗;徐英春;;異質(zhì)性萬(wàn)古霉素中介金黃色葡萄球菌:困擾我們的難題[J];中國(guó)感染與化療雜志;2013年06期

2 許宏濤;陳東科;賴惠英;胡云建;張秀珍;;異質(zhì)性萬(wàn)古霉素中介金黃色葡萄球菌的分離率調(diào)查及方法學(xué)評(píng)價(jià)[J];中華醫(yī)院感染學(xué)雜志;2012年21期

3 張紅旭;陳良安;;異質(zhì)性萬(wàn)古霉素中介的金黃色葡萄球菌耐藥機(jī)制及臨床意義[J];中華醫(yī)院感染學(xué)雜志;2012年17期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉明濤;董春花;郭洪萍;李凱述;歐陽(yáng)修河;李玉;;β-內(nèi)酰胺類抗菌藥物誘導(dǎo)異質(zhì)性萬(wàn)古霉素中介金黃色葡萄球菌及其藥敏試驗(yàn)研究[J];中華醫(yī)院感染學(xué)雜志;2017年03期

2 朱越燕;;耐甲氧西林金黃色葡萄球菌醫(yī)院感染危險(xiǎn)因素調(diào)查[J];健康研究;2016年04期

3 許燕;張宜濤;葉貴子;陸海榮;黃青山;;重組溶葡萄球菌酶成品蛋白含量測(cè)定方法的建立[J];生物技術(shù)通報(bào);2016年07期

4 劉明濤;李凱述;李超;歐陽(yáng)修河;;萬(wàn)古霉素耐藥折點(diǎn)調(diào)整對(duì)異質(zhì)性萬(wàn)古霉素中介金黃色葡萄球菌篩選方法的影響[J];中國(guó)感染與化療雜志;2016年04期

5 張爽;周昕;杜秋蓉;舒洪麗;辛力華;;2012-2014年臨床常見葡萄球菌對(duì)萬(wàn)古霉素MIC值變化分析[J];中華醫(yī)院感染學(xué)雜志;2016年14期

6 陳琦;金慧;徐虹;韋凌婭;沈林海;倪曉平;;ICU耐甲氧西林金黃色葡萄球菌分布特征分析[J];中華醫(yī)院感染學(xué)雜志;2016年11期

7 郭振秀;李凌云;;耐甲氧西林金黃色葡萄球菌(MRSA)的異質(zhì)性耐藥株的檢測(cè)方法分析[J];臨床醫(yī)藥文獻(xiàn)電子雜志;2016年14期

8 劉明濤;李超;常剛;李凱述;歐陽(yáng)修河;;異質(zhì)性萬(wàn)古霉素中介金黃色葡萄球菌分離率與檢測(cè)方法評(píng)價(jià)[J];中華醫(yī)院感染學(xué)雜志;2016年08期

9 逯曉輝;鄭港森;李s，

本文編號(hào)：1579253