【摘要】：目的：觀察不同劑量養(yǎng)陰潤(rùn)腸方含藥血清對(duì)離體培養(yǎng)的小鼠結(jié)腸Cajal間質(zhì)細(xì)胞(Interstitial Cells of Cajal ICC)凋亡和周期調(diào)控的影響,為養(yǎng)陰潤(rùn)腸方治療慢傳輸型便秘(slow transit constipation STC)提供理論依據(jù)。方法：將20只BALB/c小鼠隨機(jī)分為四組,原代分離和培養(yǎng)每組小鼠結(jié)腸組織,通過細(xì)胞流式分選技術(shù)分選出成熟的ICC,用提前制備好的正常大鼠血清及高、中、低劑量養(yǎng)陰潤(rùn)腸方含藥血清對(duì)ICC進(jìn)行分組干預(yù),干預(yù)2d后觀察ICC數(shù)量及其形態(tài)學(xué)改變,并運(yùn)用流式檢測(cè)技術(shù)評(píng)定ICC凋亡機(jī)制和細(xì)胞周期調(diào)控作用。結(jié)果：各組培養(yǎng)所得細(xì)胞經(jīng)相應(yīng)的干預(yù)后,鏡下觀察后統(tǒng)計(jì)高、中、低及空白劑量組細(xì)胞平均計(jì)數(shù)分別為(13.94±2.64；10.17±1.33；8.17±1.47；6.33±1.63),高劑量和中劑量組細(xì)胞數(shù)量較空白組表達(dá)升高(P0.01),低劑量組與—表達(dá)無明顯差異(P0.05),且低劑量組與高劑量組存在統(tǒng)計(jì)學(xué)差異(P0.01),中劑量組與高劑量組和低劑量組之間無統(tǒng)計(jì)學(xué)差異(P0.05)。在細(xì)胞凋亡作用上,早凋亡和晚凋亡之總和為總凋亡率,空白組、養(yǎng)陰潤(rùn)腸方含藥血清高、中、低劑量組早期和晚期凋亡和為16.9%、9.5%、12.4%、12.6%,總凋亡率：空白組低劑量組中劑量組高劑量組。養(yǎng)陰潤(rùn)腸方血清能有效減少cajal細(xì)胞凋亡。細(xì)胞周期調(diào)控作用中,空白組、養(yǎng)陰潤(rùn)腸方含藥血清高、中、低劑量組S期和G2期細(xì)胞總和所占比分別為36.52%、41.02%、43.86%、82.40%,含藥血清組內(nèi)正處于復(fù)制活動(dòng)期的ICC數(shù)量所占比例高劑量組中劑量組低劑量組空白劑量組。結(jié)論：養(yǎng)陰潤(rùn)腸方能促進(jìn)ICC數(shù)量的增長(zhǎng),并能有效地減少ICC凋亡數(shù)量和提高細(xì)胞分裂能力及活性,其通便機(jī)制可能為通過促進(jìn)ICC的數(shù)量生長(zhǎng)及減少其凋亡而達(dá)到促進(jìn)腸動(dòng)力,為治療STC尋求一條新的途徑。
[Abstract]:Objective: to observe the effect of different doses of Yangyin Runchang recipe containing serum on (Interstitial Cells of Cajal ICC) apoptosis and cycle regulation of mouse colon Cajal Leydig cells in vitro. It provides a theoretical basis for Yangyin Runchang recipe in the treatment of slow transit constipation (slow transit constipation STC). Methods: 20 BALB/c mice were randomly divided into four groups. The colon tissue of each group was isolated and cultured. Mature ICC, was separated by flow cytometry and normal rat serum was prepared in advance. The serum containing low dose Yangyin Runchang recipe was divided into groups to intervene ICC. After 2 days of intervention, the number and morphological changes of ICC were observed, and the apoptosis mechanism and cell cycle regulation of ICC were evaluated by flow cytometry. Results: after the corresponding intervention, the average cell count of the cultured cells in the middle, low and blank dose groups was (13.94 鹵2.64) 鈮，

本文編號(hào)：2484912