麥粒灸對阿霉素毒性心肌病的效應(yīng)觀察及其抗氧化應(yīng)激機制研究
發(fā)布時間：2018-10-21 13:00
【摘要】：目的:觀察麥粒灸對阿霉素(Adriamycin,ADR)所致毒性心肌病模型大鼠的效應(yīng)及其抗氧化應(yīng)激作用的可能機制。方法:將60只無特定病原體(Specific pathogen Free,SPF)級潑累格·多雷(Sprague-Dawley,SD)大鼠隨機分為空白組、ADR毒性心肌病模型對照組(即模型組)及ADR+麥粒灸組(即麥粒灸組),每組20只[1]。各組相同環(huán)境飼養(yǎng),模型組及麥粒灸組予尾靜脈注射ADR造模;空白組尾靜脈注射等量的生理鹽水,不施灸,只接受與其它兩組相同方式的抓取固定;麥粒灸組在給藥的同時取"內(nèi)關(guān)"、"巨闕"穴進行麥粒灸治療[1]。共持續(xù)4周[1]。末次治療結(jié)束 24h 后測定大鼠心率(heart rate,HR)、左心室收縮壓(left ventricular systolic pressure,LVSP)、左心室舒張壓(left ventricular diastolic pressure,LVDP)、等容收縮期左心室內(nèi)壓最大上升速率(+dp/dtmax)、等容舒張期左心室內(nèi)壓最大下降速率(-dp/dtmax)、左室內(nèi)壓變化時間(t-dp/dtmax)評價心功能[1];麻醉,取各組大鼠血樣及心臟,光學顯微鏡下察看心肌病理形態(tài)結(jié)構(gòu)改變[1];制備血清及心肌組織勻漿,酶聯(lián)免疫法(enzyme-linked immuno sorbent assay,ElISA)檢測血清中的乳酸脫氫酶(lactate dehydrogenase,LDH)、磷酸肌酸激酶(creatine kinase,CK)活性及心肌cTnI濃度;心肌組織中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)的活性[1]。結(jié)果:(1)模型組的HR、LVSP、+dp/dtmax、-dp/dtmax均較空白組及麥粒灸組低,LVDP和t-dp/dtmax則升高,差異有統(tǒng)計學意義(P0.05);與模型組比較,麥粒灸組HR、LVSP、+dp/dtmax、-dp/dtmax升高,LVDP 和 t-dp/dtmax降低,差異有統(tǒng)計學意義(P0.05)。(2)與空白組比較,模型組的心肌組織光鏡下呈ADR心肌病樣改變:細胞腫脹充血、肌纖維部分溶解且排列紊亂,白細胞浸潤明顯;與模型組比較,麥粒灸組光鏡下心肌細胞輕微腫脹、出血,白細胞浸潤不明顯[1]。(3)模型組、麥粒灸組大鼠血清LDH、CK酶活性,心肌cTnI濃度及MDA活性均較空白組顯著升氋(P0.01),心肌SOD活性則顯著下降(P0.05);與模型組比較,麥粒灸組大鼠血清LDH、CK酶活性、心肌cTnI濃度及MDA活性顯著降低(P0.01),心肌SOD活性顯著升高(P0.05)。結(jié)論:麥粒灸可提高心肌收縮能力,改善心功能,減輕ADR引起的心肌細胞腫脹、充血及炎癥反應(yīng),顯著降低血清LDH、CK活力,cTnI濃度及心肌組織MDA活性,同時顯著升高心肌組織中SOD的活性,實現(xiàn)對ADR毒性心肌病大鼠的抗氧化應(yīng)激作用。其機制可能與麥粒灸提高SOD活性,同時降低MDA活性來減輕過氧化產(chǎn)物對心肌的損傷,進而最終調(diào)控相關(guān)的抗氧化信號通路來發(fā)揮心肌保護效應(yīng)有關(guān)。
[Abstract]:Aim: to observe the effect of grain moxibustion on adriamycin (Adriamycin,ADR) induced cardiomyopathy model rats and the possible mechanism of antioxidant stress. Methods: sixty Sprague-Dawley,SD rats with no specific pathogen (Specific pathogen Free,SPF) were randomly divided into blank group, model group (model group) and ADR moxibustion group (20 rats in each group). Each group was fed in the same environment, the model group and the wheat grain moxibustion group were injected with ADR by caudal vein, and the blank group was injected with the same amount of normal saline, without moxibustion, only accepted grasping and fixing in the same way as the other two groups. Grain moxibustion group was treated with grain moxibustion at the same time as "Neiguan" and "Juque". It lasted for 4 weeks [1]. Heart rate (heart rate,HR), left ventricular systolic pressure (left ventricular systolic pressure,LVSP), left ventricular diastolic pressure (left ventricular diastolic pressure,LVDP), maximum rate of left ventricular pressure rise (dp/dtmax) during isovolumic systolic period (Isovolumic diastolic phase), maximum rate of decrease of left ventricular pressure during isovolumic diastolic period (IVDD) were measured 24 hours after the end of the last treatment. Rate (- dp/dtmax), left ventricular pressure change time (t-dp/dtmax) to evaluate cardiac function [1]. Blood samples and heart samples were taken from each group of rats, and the morphologic changes of cardiomyopathy were observed under optical microscope [1]. Serum and myocardial tissue homogenate were prepared. The activities of lactate dehydrogenase (lactate dehydrogenase,LDH), creatine phosphokinase (creatine kinase,CK), myocardial cTnI and the activities of superoxide dismutase (superoxide dismutase,SOD) and malondialdehyde (malondialdehyde,MDA) were detected by enzyme linked immunosorbent assay (enzyme-linked immuno sorbent assay,ElISA). Results: (1) the HR,LVSP, dp/dtmax,-dp/dtmax of the model group was lower than that of the blank group and the wheat grain moxibustion group, while the LVDP and t-dp/dtmax levels were higher than those of the control group (P0.05), and compared with the model group, the HR,LVSP, dp/dtmax,-dp/dtmax, LVDP and t-dp/dtmax in the model group were significantly higher than those in the control group (P0.05). (2). The myocardial tissue of the model group showed ADR cardiomyopathy changes under light microscope: the cells were swollen and congested, the muscle fibers were disintegrated and arranged disorderly, the infiltration of white blood cells was obvious, compared with the model group, the myocardial cells in the moxibustion group were slightly swollen and bleeding under light microscope. (1) WBC infiltration was not obvious [1]. (3) Serum LDH,CK enzyme activity, myocardial cTnI concentration and MDA activity were significantly increased in model group and wheat grain moxibustion group compared with blank group (P0.01), and myocardial SOD activity decreased significantly (P0.05), compared with model group, LDH,CK enzyme activity in serum of wheat grain moxibustion group was significantly higher than that of control group (P0.01). Myocardial cTnI concentration and MDA activity decreased significantly (P0.01), myocardial SOD activity increased significantly (P0.05). Conclusion: grain moxibustion can improve myocardial contractility, improve cardiac function, reduce myocardial cell swelling, congestive and inflammatory reaction induced by ADR, decrease serum LDH,CK activity, cTnI concentration and MDA activity in myocardial tissue. At the same time, the activity of SOD in myocardial tissue was significantly increased, and the antioxidant stress was achieved in ADR induced cardiomyopathy rats. The mechanism may be related to the increase of SOD activity by grain moxibustion and the decrease of MDA activity in order to reduce the myocardial injury caused by peroxidation products and ultimately regulate the related antioxidant signaling pathway to exert myocardial protection effect.
【學位授予單位】：南京中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245
本文編號：2285160
[Abstract]:Aim: to observe the effect of grain moxibustion on adriamycin (Adriamycin,ADR) induced cardiomyopathy model rats and the possible mechanism of antioxidant stress. Methods: sixty Sprague-Dawley,SD rats with no specific pathogen (Specific pathogen Free,SPF) were randomly divided into blank group, model group (model group) and ADR moxibustion group (20 rats in each group). Each group was fed in the same environment, the model group and the wheat grain moxibustion group were injected with ADR by caudal vein, and the blank group was injected with the same amount of normal saline, without moxibustion, only accepted grasping and fixing in the same way as the other two groups. Grain moxibustion group was treated with grain moxibustion at the same time as "Neiguan" and "Juque". It lasted for 4 weeks [1]. Heart rate (heart rate,HR), left ventricular systolic pressure (left ventricular systolic pressure,LVSP), left ventricular diastolic pressure (left ventricular diastolic pressure,LVDP), maximum rate of left ventricular pressure rise (dp/dtmax) during isovolumic systolic period (Isovolumic diastolic phase), maximum rate of decrease of left ventricular pressure during isovolumic diastolic period (IVDD) were measured 24 hours after the end of the last treatment. Rate (- dp/dtmax), left ventricular pressure change time (t-dp/dtmax) to evaluate cardiac function [1]. Blood samples and heart samples were taken from each group of rats, and the morphologic changes of cardiomyopathy were observed under optical microscope [1]. Serum and myocardial tissue homogenate were prepared. The activities of lactate dehydrogenase (lactate dehydrogenase,LDH), creatine phosphokinase (creatine kinase,CK), myocardial cTnI and the activities of superoxide dismutase (superoxide dismutase,SOD) and malondialdehyde (malondialdehyde,MDA) were detected by enzyme linked immunosorbent assay (enzyme-linked immuno sorbent assay,ElISA). Results: (1) the HR,LVSP, dp/dtmax,-dp/dtmax of the model group was lower than that of the blank group and the wheat grain moxibustion group, while the LVDP and t-dp/dtmax levels were higher than those of the control group (P0.05), and compared with the model group, the HR,LVSP, dp/dtmax,-dp/dtmax, LVDP and t-dp/dtmax in the model group were significantly higher than those in the control group (P0.05). (2). The myocardial tissue of the model group showed ADR cardiomyopathy changes under light microscope: the cells were swollen and congested, the muscle fibers were disintegrated and arranged disorderly, the infiltration of white blood cells was obvious, compared with the model group, the myocardial cells in the moxibustion group were slightly swollen and bleeding under light microscope. (1) WBC infiltration was not obvious [1]. (3) Serum LDH,CK enzyme activity, myocardial cTnI concentration and MDA activity were significantly increased in model group and wheat grain moxibustion group compared with blank group (P0.01), and myocardial SOD activity decreased significantly (P0.05), compared with model group, LDH,CK enzyme activity in serum of wheat grain moxibustion group was significantly higher than that of control group (P0.01). Myocardial cTnI concentration and MDA activity decreased significantly (P0.01), myocardial SOD activity increased significantly (P0.05). Conclusion: grain moxibustion can improve myocardial contractility, improve cardiac function, reduce myocardial cell swelling, congestive and inflammatory reaction induced by ADR, decrease serum LDH,CK activity, cTnI concentration and MDA activity in myocardial tissue. At the same time, the activity of SOD in myocardial tissue was significantly increased, and the antioxidant stress was achieved in ADR induced cardiomyopathy rats. The mechanism may be related to the increase of SOD activity by grain moxibustion and the decrease of MDA activity in order to reduce the myocardial injury caused by peroxidation products and ultimately regulate the related antioxidant signaling pathway to exert myocardial protection effect.
【學位授予單位】：南京中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245
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