【摘要】：目的:探討回藥臭殼蟲(CKC)含藥血清對人肺鱗癌A549細胞生長的抑制作用及對細胞形態(tài)的影響。方法:將40只雄性SD大鼠按體重隨機分為生理鹽水對照組(NS)、回藥臭殼蟲低劑量組(CKCL)、回藥臭殼蟲中劑量組(CKCM)、回藥臭殼蟲高劑量組(CKCH)各10只,分別給予相應(yīng)的CKCL、CKCM、CLCH濃縮水煎劑灌胃,NS組給予等量生理鹽水灌胃,連續(xù)5天。采用血清藥理學(xué)方法,制備含藥血清;體外培養(yǎng)人肺腺癌A549細胞,將細胞分為實驗組與對照組,實驗組分別給予10%、20%濃度的相應(yīng)組大鼠含藥血清,對照組給予相同濃度對照組大鼠血清,培養(yǎng)24h、48h、72h后觀察細胞形態(tài)變化,采用MTT法檢測不同濃度含藥血清對A549細胞的生長抑制率。結(jié)果:光鏡下觀察發(fā)現(xiàn),各濃度臭殼蟲含藥血清組細胞形態(tài)不規(guī)則,胞膜出現(xiàn)不同程度的回縮,胞核固縮,細胞間距增大,其中以20%濃度臭殼蟲含藥血清高劑量組作用72h最為明顯。MTT法檢測細胞生長抑制率發(fā)現(xiàn),各實驗組不同濃度抑制率均高于對照組(P0.05);回藥臭殼蟲高劑量組72h抑制率高于低劑量組(P0.05);回藥臭殼蟲各劑量組72h抑制率高于24h抑制率(P0.05)。結(jié)論:回藥臭殼蟲含藥血清可抑制A549細胞的生長增殖。
[Abstract]:Aim: to investigate the inhibitory effect of (CKC) serum on the growth of human lung squamous cell carcinoma cell line A549 and its effect on cell morphology. Methods: forty male SD rats were randomly divided into normal saline control group (n = 10), (NS), control group (n = 10), low dose (CKCL), anther group (n = 10), high dose (CKCM), group (n = 10) and (CKCH) group (n = 10). The rats in the NS group were given the same amount of normal saline for 5 days. Human lung adenocarcinoma cell line A549 was cultured in vitro. The cells were divided into experimental group and control group. The experimental group was given 10% 20% corresponding rat serum. The control group was treated with the same concentration of rat serum and cultured for 24 h or 48 h for 72 h. The growth inhibition rate of A549 cells was detected by MTT assay. Results: under light microscope, it was found that the cell morphology was irregular, the cell membrane was retracted to varying degrees, the nucleus was pyknosis and the cell spacing was increased. Among them, the high dose group with 20% drug serum had the most obvious effect for 72 hours. The inhibition rate of cell growth was detected by MTT assay. The inhibition rate of different concentrations in each experimental group was higher than that of the control group (P0.05); the inhibition rate of 72h in high-dose group was higher than that in low-dose group (P0.05); the inhibition rate of 72h in each dose group was higher than that in 24h (P0.05). Conclusion: the drug serum can inhibit the growth and proliferation of A549 cells.
【作者單位】： 寧夏醫(yī)科大學(xué);寧夏醫(yī)科大學(xué)附屬回醫(yī)中醫(yī)院;
【基金】：寧夏回族自治區(qū)自然科學(xué)基金資助項目(NZ14096) 寧夏醫(yī)科大學(xué)科學(xué)基金資助項目優(yōu)勢學(xué)科群(XY201422)
【分類號】：R29
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本文編號：2273642