本文選題：電針 + 酒精�。� 參考：《新鄉(xiāng)醫(yī)學院》2017年碩士論文

【摘要】：目的針灸是祖國傳統醫(yī)學的瑰寶,在臨床多種疾病的治療和預防中有著廣泛的應用,電針的發(fā)明為現代醫(yī)學研究針灸的治療作用和機制提供了科學的手段。本實驗研究了2/100Hz電針能否治療小鼠酒精依賴戒斷期的抑郁狀態(tài),能否翻轉小鼠酒精依賴戒斷期海馬腦區(qū)腦源性神經營養(yǎng)因子和神經發(fā)生的減少。方法把48只小鼠分為蒸餾水組、酒精依賴戒斷組、束縛組和2/100Hz電針組。首先建立小鼠酒精依賴模型,采用雙瓶自由選擇飲酒的方法進行酒精依賴造模,接下來是14天的自然戒斷,這段時間內給予電針治療。電針組電針每天1次,8:00a.m.,頻率2/100 Hz,強度分別為0.5、1.0、1.5 mA,三個強度的時間各為10min;束縛組每天1次,8:00a.m.,30min;自然戒斷組動物不做任何處理,常規(guī)飼養(yǎng)。以上各組動物每組12只,43天時全部進行強迫游泳實驗,檢測動物抑郁狀態(tài),44天時選擇6只給予BrdU處理(100 mg/kg,i.p.),2小時之后灌流取材,進行免疫組化實驗,檢測神經發(fā)生;另外6只直接處死取材,進行ELISA實驗檢測海馬腦源性神經營養(yǎng)因子含量。結果1.經過28天的雙瓶自由選擇飲酒實驗,檢測小鼠的酒精偏愛率和飲酒量,我們的結果表明每只小鼠的酒精偏愛率都達到了75%以上,酒精攝入量都達到了15g/kg以上,對酒精形成了明顯的偏愛,而且三組小鼠對酒精的偏愛率和酒精攝入量沒有統計學差異(P0.05),同時我們每周檢測小鼠體重,結果表明和蒸餾水組相比,飲酒對小鼠的體重沒有影響(P0.05)。2.強迫游泳實驗顯示,酒精戒斷14天后,和蒸餾水組相比,酒精戒斷組的小鼠處于抑郁狀態(tài),強迫游泳的不動時間明顯延長(P㩳0.01)。ELISA實驗結果顯示,酒精戒斷后14天,和蒸餾水組相比,戒斷組小鼠海馬腦區(qū)腦源性神經營養(yǎng)因子的含量明顯減少(P㩳0.05)。BrdU免疫組化結果顯示,和蒸餾水組相比,酒精戒斷組小鼠海馬神經發(fā)生減少(P㩳0.05)。3.在戒斷期給予小鼠2/100Hz電針治療,強迫游泳實驗中可以明顯減少小鼠游泳時的不動時間(P㩳0.05),表明電針可以改善小鼠酒精依賴戒斷期的抑郁狀態(tài)。ELISA實驗顯示電針可以增加海馬腦區(qū)腦源性神經營養(yǎng)因子的含量(P㩳0.05),BrdU免疫組化結果顯示,2/100Hz電針可以促進海馬神經細胞發(fā)生的增加(P㩳0.05)。結論1.雙瓶自由選擇飲酒14天后小鼠表現出抑郁樣行為,減少小鼠海馬腦源性神經營養(yǎng)因子含量和海馬神經發(fā)生。2.2/100Hz電針可以改善酒精依賴戒斷期的抑郁,增加海馬腦源性神經營養(yǎng)因子含量和神經發(fā)生。
[Abstract]:Objective Acupuncture and moxibustion is a treasure of traditional Chinese medicine, which is widely used in the treatment and prevention of many kinds of clinical diseases. The invention of electroacupuncture provides a scientific means for modern medicine to study the therapeutic effect and mechanism of acupuncture and moxibustion. This experiment was conducted to study whether 2/100Hz electroacupuncture could treat depression in alcohol dependence withdrawal period in mice and reverse the decrease of brain-derived neurotrophic factor and neurogenesis in hippocampus of mice during alcohol dependence withdrawal period. Methods 48 mice were divided into distilled water group, alcohol dependent withdrawal group, bound group and 2/100Hz electroacupuncture group. The model of alcohol dependence in mice was established first, and the model was made with the method of double bottles of free choice. Then, the natural withdrawal of 14 days was given, and electroacupuncture was given during this period. In the electroacupuncture group, once a day, 8: 00a.m., with a frequency of 2 / 100 Hz, with the intensity of 0.5 脳 1.0 Ma and 1.5 Ma, the time of the three intensities was 10 min, and the bound group was 8: 00 a.m..30 min. once a day. The animals in the natural abstinence group did not do any treatment and were raised routinely. In each group, 12 animals in each group were subjected to forced swimming for 43 days, and 6 animals were given BrdU for 44 days. After 2 hours of BrdU treatment, the animals were perfused with 100 mg / kg i.p. for 2 hours, and the neurogenesis was detected by immunohistochemistry. The other 6 rats were killed directly and the content of brain-derived neurotrophic factor (BDNF) in hippocampus was detected by ELISA test. Result 1. After 28 days of free choice of alcohol in two bottles, the alcohol preference rate and alcohol consumption of mice were measured. Our results showed that the alcohol preference rate of each mouse was more than 75%, and the alcohol intake was above 15g/kg. There was no significant difference in alcohol preference rate and alcohol intake among the three groups. At the same time, we measured the body weight of mice every week. The results showed that alcohol consumption had no effect on the body weight of mice compared with distilled water group. The forced swimming test showed that after 14 days of alcohol withdrawal, the mice in the alcohol withdrawal group were in a depressed state compared with the distilled water group, and the immobility time of forced swimming was significantly prolonged. The results of Elisa showed that 14 days after alcohol withdrawal, compared with the distilled water group, the immobility time of forced swimming group was significantly longer than that of the distilled water group. The content of brain-derived neurotrophic factor (BDNF) in the hippocampus of abstinent group was significantly decreased. The results of immunohistochemistry showed that compared with distilled water group, the hippocampal neurogenesis of alcohol abstinent group was decreased as compared with that of distilled water group. During the abstinence period, mice were treated with 2/100Hz electroacupuncture, In forced swimming experiment, the immobility time of swimming in mice was significantly reduced, indicating that electroacupuncture could improve the depression state of mice during alcohol dependence withdrawal period. Elisa experiment showed that electroacupuncture could increase brain-derived neurotrophic factor in hippocampal brain area. The results of BrdU immunohistochemistry showed that 2 / 100 Hz electroacupuncture could promote the increase of hippocampal neurogenesis. Conclusion 1. After 14 days of drinking, the mice showed depressive behavior. Reducing the content of brain-derived neurotrophic factor in hippocampus and hippocampal neurogenesis. 2.2 / 100 Hz electroacupuncture could improve depression in alcohol dependence withdrawal period. Increase the content of brain-derived neurotrophic factor and neurogenesis in hippocampus.
【學位授予單位】：新鄉(xiāng)醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245

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