本文選題：錫葉藤 + 沒食子酸��； 參考：《中華中醫(yī)藥學(xué)刊》2017年01期

【摘要】：目的:建立錫葉藤藥材的質(zhì)量標(biāo)準(zhǔn)。方法:對錫葉藤根的性狀、顯微進(jìn)行鑒別;采用薄層色譜法(TLC)對錫葉藤進(jìn)行定性鑒別;采用HPLC法測定藥材中的沒食子酸含量,色譜柱為Phenomenex C18柱(4.6 mm×250 mm,5μm),流動相為甲醇-0.1%磷酸水溶液(5∶95),檢測波長280 nm,流速1 m L·min-1,柱溫30℃。結(jié)果:錫葉藤根的薄層鑒別鑒別特征明顯,專屬性強(qiáng);沒食子酸的進(jìn)樣量在0.026 8~1.342μg(r=0.999 9)范圍內(nèi)與峰面積積分值呈良好線性關(guān)系,平均加樣回收率為99.65%(RSD=1.26%,n=6)。結(jié)論:所建標(biāo)準(zhǔn)可用于錫葉藤藥材的質(zhì)量控制。
[Abstract]:Objective: to establish the quality standard of Dioscorea tinctata.Methods: TLC was used to identify the characters of the root, TLC was used to determine the content of Gallic acid, and HPLC was used to determine the content of Gallic acid.The chromatographic column was Phenomenex C18 column (4.6mm 脳 250mm-1), the mobile phase was methanol-0.1% phosphoric acid aqueous solution (5: 95), the detection wavelength was 280nm, the flow rate was 1 mL 路min ~ (-1), the column temperature was 30 鈩，

本文編號：1749194